Identification of Rhizo-bacterium Inhibiting Diaporthe citri Causing Citrus Melanose

감귤 검은점무늬병균의 생육을 저해하는 근권 세균의 분리 및 동정

  • Published : 2009.12.31


Rhizo-bacteria were isolated from organic-farming soils to select antagonistic agent for controlling citrus melanose disease. Among several antagonistic bacteria, KB-401 effectively inhibited mycelial growth of several plant fungal pathogens, including the pathogen of citrus melanose, Diaporthe citri. KB-401 also inhibited spore germination of the fungal pathogen. The tip of germ tube was swollen when conidia of D. citri were co-culture with KB-401 in PD broth amended 1% glucose. KB-401 was identified as Bacillus subtilis through the investigation for physiological characters and the analysis of nucleotide sequences of 16S rDNA.


  1. Arrebola, E., D. Sivakumar and L. Korsten (2010) Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus. in press
  2. Leelasuphakul, W., P. Hemmanee and S. Chuenchitt (2008) Growth inhibitory properties of Bacillus subtilis strains and their metabolites against the green mold pathogen (Penicillium digitatum Sacc.) of citrus fruit. Postharvest Biol. Technol. 31:1-8
  3. 권혁모, 남기웅, 김광식, 김동환, 이성찬, 현재욱 (2003) 감귤나무 가지에서 분리한 검은점무늬병균 Diaporthe citri의 균학적 특징. 식물병연구 8:153-158
  4. 정우철, 신택수, 도기석, 김원극, 이재호, 최기현 (2006) 잔디 피시움 마름병(Pythium blight)의 생물학적 방제를 위한 길항 미생물의 선발과 효력 검정. 식물병연구 12:260-266
  5. 허길현, 박서기 (2005a) 전남지역 유자과원에서의 검은점무늬병 발생 양상. 식물병연구 11:10-15
  6. 현재욱, 김동환, 김광식, 이성찬, 고상욱, 임한철 (2004) 최근 부지화 감귤 품종에 발생하는 식물병의 종류 및 그 증상. 식물병연구 10:94-99
  7. Bushong, P. M. and L. W. Timmer (2000) Evaluation of postinfection control of citrus scab and melanose with benomyl, fenbuconazole, and azoxystrobin. Plant Dis. 84:1246-1249
  8. Johnson, J. L. (1994) Similarity analysis of rRNAs. In P. Gerhardt, R.G.E. Murray, W.A. Wood, and N.R. Krirg (eds.). Methods for general and molecular bacteriology, p. 683-700. American Society for Microbiology, Washington, D.C., USA
  9. Singh, V. and B. J. Deverall (1984) Bacillus subtilis as a control agent against fungal pathogens of citrus fruit. Tran. Br. Mycol. Soc. 83:487-490
  10. 김한모, 이광렬, 백정우, 김현주, 박종영, 이진우, 정순재, 문병주 (2004) 결구상추(Sclerotinia sclerotiorum)에 대한 길항세균의 분리 및 동정. 식물병연구 10:331-336
  11. 허길현, 박서기 (2005b) 전남 지역 유자과원의 검은점무늬병균 포자 형성과 비산. 식물병연구 11:16-20
  12. Dhingra, O. D., E. S. G. Mizubuti and F. M. Santana (2003) Chaetomium globosum for reducing primary inoculum of Diaporthe phaseolorum f. sp. meridionalis in soil-surface soybean stubble in field conditions. Biological Control 26: 302-310
  13. Sharma, R. R., D. Singh and R. Singh (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control 50:205-221
  14. 정우철, 신택수, 김봉수, 임재성, 이재호, 김진원 (2008) 잔디 갈색퍼짐병(Large patch)의 생물학적 방제를 위한 길항 미생물의 선발 및 효력 검정. 식물병연구 14:43-50
  15. 김경희, 임명택, 허재선, 염규진, 고영진 (2008) 미생물제제를 이용한 차나무 겹둥근무늬병의 방제. 식물병연구 14:37-42
  16. Agostini, J. P., P. M. Bushong and L. W. Timmer (2003b) Greenhouse evaluation of products that induce host resistance for control of scab, melanose, and Alternaria brown spot of citrus. Plant Dis. 87:69-74
  17. Hang, N. T. T., S-O. Oh, G. H. Kim, J-S. Hur and Y. J. Koh (2005) Bacillus subtilis S1-0210 as a biocontrol agent against Botrytis cinerea in strawberries. Plant Pathol. J. 21:59-63
  18. Lane, D. J. (1991) 16S/23S rRNA sequencing. In E. Stackebrandt and M. Goodfellow (eds.). Nucleic acid techniques in bacterial systematics, pp. 115-175. John Wiley and Sons, Chichester, UK
  19. 현재욱, 고상욱, 김동환, 한승갑, 김광식, 권혁모, 임한철 (2005) 친환경적 감귤 병 방제를 위한 구리제의 효율적 사용. 식물병연구 11:115-121
  20. Agostini, J. P., P. M. Bushong, A. Bhatia and L. W. Timmer (2003a) Influence of environmental factors on severity of citrus scab and melanose. Plant Dis. 87:1102-1106