돌연변이 식물 및 형질전환된 효모에서 phytochelatin synthase 발현이 살균제 tolclofos-methyl 분해에 미치는 영향

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윤하임;김장억;신재호;김정회;이상만
Yoon, Ha-Im;Kim, Jang-Eok;Shin, Jae-Ho;Kim, Jeong-Hoe;Lee, Sang-Man

  • 발행 : 2009.12.30

초록

Phytochelatins (PCs) are small-sized peptides synthesized by PC synthase (PCS) using glutathione (GSH) as a substrate, and they play an important role in the detoxification of toxic heavy metals in plants, fission yeast, and other living organisms. Recently, it has been suggested that PCS is also involved in degradation of some xenobiotics including monobromobimane. PCS cleaves the Gly residue from GSH-xenobiotics conjugates resulting in ${\gamma}$-Glu-Cys-xenobiotics, and this is to degraded further. Therefore, our research is focus on whether PCS is also involved in degradation of tolclofos-methyl, an important pesticide which has been used in ginseng cultivated areas. Heterologous expression of Arabidopsis PCS confers tolerance to tolclofos-methyl in yeast. Furthermore, PCS-deficient Cad1-3 Arabidopsis mutant showed high sensitivity to tolclofos-methyl compared with wild-type plants. These results imply that PCS is involved in degradation of tolclofos-methyl as other xenobiotics.

키워드

Ginseng;glutathione;pesticide;phytochelatin synthase;xenobiotics

참고문헌

  1. Rauser, W. E. (1990) Phytochelatins. Annu. Rev. Biochem. 59, 61-86 https://doi.org/10.1146/annurev.bi.59.070190.000425
  2. Cobbett, C. S. (2000) Phytochelatin biosynthesis and function in heavy-metal detoxification. Cur. Opin. Plant Biol. 3, 211-216 https://doi.org/10.1016/S1369-5266(00)80067-9
  3. Grill, E., Loffler, S., Winnacker, E. L., and Zenk, M. H. (1989) Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific γ-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase). Proc. Natl. Acad. Sci. USA 86, 6838-6842 https://doi.org/10.1073/pnas.86.18.6838
  4. Meister, A. (1995) Glutathione metabolism, Methods Enzymol. 251, 3-7 https://doi.org/10.1016/0076-6879(95)51106-7
  5. Beck, A., Lendzian, K., Oven, M., Christmann, A., and Grill, E. (2003) Phytochelatin synthases catalyzes key step in turnover of glutathione conjugates, Phyochemistry 62, 423-431 https://doi.org/10.1016/S0031-9422(02)00565-4
  6. Grzam, A., Pierre, T., Clemense, S., Hell, R., and Meyer, A. J. (2006) Vacuolar sequestration of glutathione S-conjugates outcompetes a possible degradation of the glutathione moiety by phytochelatin synthase, FEBS Lett. 580, 6384-6390 https://doi.org/10.1016/j.febslet.2006.10.050
  7. Blum, R., Beck, A., Korte, A., Stengel, A., Letzel, T., Lendzian, K., and Grill, E. (2007) Function of phytochelatin synthase in catabolism of glutathioneconjugates, Plant J. 49, 740-749 https://doi.org/10.1111/j.1365-313X.2006.02993.x
  8. Keiko, I-S., Takuo, F., Toshiyuki, K., and Takimoto, Y. (2004) Metabolism of tolclofos-methyl in lettuce (Lactuca sativa), J. Pestic. Sci. 29, 322-327 https://doi.org/10.1584/jpestics.29.322
  9. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) In Molecular Cloning: A Laboratory manual, (2nd ed.). Cold Spring Harbor Laboratory Press, New York, NY, U.S.A
  10. Lee, S. and Kang, B. S. (2005) Phytochelatin is not a primary factor in determining copper tolerance. J. Plant Biol. 48, 32-38 https://doi.org/10.1007/BF03030562