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Isolation and Characterization of Bacillus cereus A-139 Producing Auxin from East Coast Sand Dunes

동해안 사구로부터 Auxin을 생산하는 Bacillus cereus A-139의 분리 및 그 특성

  • Published : 2009.12.30

Abstract

A bacterium, which was named to be Bacillus cereus A-139, secreting auxin was isolated from the east coast sand dunes in Korea. The secretion of auxin was confirmed by HPLC. When cultured in LB broth, Bacillus cereus A-139 produced $16.12\;{\mu}$g/mL auxin after 8 days in LB broth. Bacillus cereus A-139 produced $49\;{\mu}$g/mL auxin and $162.6\;{\mu}$g/mL by the addition of 2% tryptone and 0.1% tryptophan, respectively. The root growth of Arabidopsis thaliana was retarded by Bacillus cereus A-139 culture broth up to 57% but the formation of lateral roots was increased up to almost twice after 4 days incubation. Also the formation of lateral roots of mung bean was increased up to 57% after 10 days incubation.

Keywords

Auxin;Bacillus cereus;Root growth retardation;Sand Dunes

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