A Ternary Microfluidic Multiplexer using Control Lines with Digital Valves of Different Threshold Pressures

서로 다른 임계압력을 가지는 디지털 밸브가 설치된 제어라인을 이용한 3 진 유체분배기

  • 이동우 (한국과학기술원 바이오및뇌공학과, 디지털나노구동연구단) ;
  • 조영호 (한국과학기술원 바이오및뇌공학과 및 기계공학과, 디지털나노구동연구단)
  • Published : 2009.06.01


We present a ternary microfluidic multiplexer unit, capable to address three flow channels using a pair of control lines with two different threshold pressure valves. The previous binary multiplexer unit addresses only two flow channels using a pair of control line with identical threshold pressure valves, thus addressing $2^{n/2}$ flow channels using n control lines. The present ternary multiplexer addressing three flow channels using a pair of control lines, however, is capable to address $3^{n/2}$ flow channels using n control lines with two different threshold pressure valves. In the experimental study, we characterized the threshold pressure and the response time of the valves used in the ternary multiplexer. From the experimental observation, we also verified that the present ternary multiplexer unit could be operated by two equivalent valve operating conditions: the different static pressures and dynamic pressures at different duty ratio. And then, $3{\times}3$ well array stacking ternary multiplexers in serial is addressed in cross and plus patterns, thus demonstrating the individual flow channel addressing capability of the ternary multiplexer. Thus, the present ternary multiplexer reduces the number of control lines for addressing flow channels, achieving the high well control efficiency required for simple and compact microfluidic systems.


Microfluidic Multiplexer;Pressure Valve of Different Threshold;Ternary Multiplexer;Digital Valve


  1. King, K. R., Wang, S., Irimia, D., Jayaraman, A., Toner, M. and Yarmush, M. L., 2006, 'A High-Throughput Microfluidic Real-Time Gene Expression Living Cell Array,' Lab on a chip, Vol. 7, pp. 77-85
  2. Sato, K., Yamanaka, M., Takahashi, H., Tokeshi, M., Kimura, H. and Kitamori, T., 2002, 'Microchip-Based Immunoassay system with Branching Multichannels for Simultaneous Determination of Iterferon-g ,' Electrophoresis, Vol. 23, pp. 734-739<734::AID-ELPS734>3.0.CO;2-W
  3. Urbanski, J. P., Thies, W., Rhodes, C., Amarasinghe, S. and Thorsen, T., 2006, 'Digital Microfluidics using Soft Lithography,' Lab on a chip, Vol. 6, pp. 96-104
  4. Melin, J. and Quake, S. R., 'Microfluidic Large-Scale Integratioin: The Evolution of Design Rules for Biological Automation,' Annu. Rev. Biophys. Biomol. Struct. , 2007, pp. 213-231
  5. Grover, W. H., Ivester, R. H. C., Jensen, E. C. and Mathies, R. A., 'Development and Multiplexed Control of Latching Pneumatic Valves using Microfluidic Logical Structures,' Lab on a chip, Vol. 6(2006), pp. 623-631
  6. Hua, Z., Xia, Y., Srivannavit, O., J-. Rouillard, M., Zhou, X., Gao, X. and Gulari, E., 'A Versatile Microreactor Platform Featuring a Chemical-Resistant Microvalve Array for Addressable Multiplex Syntheses and Assays,' J. Micromech. Microeng., Vol. 16(2006), pp. 1433-1443
  7. Wang, J., Sui, G., Mocharla, V. P., Lin, R. J., Phelps, M. E., Kolb, H. C. and Tseng, H-. R., 2006, 'Integrated Microfluidics for Parallel Screening of an In Situ Click Chemistry Library,' Angew. Chem. Int. Ed., Vol. 45, pp. 5276-5281
  8. Thorsen, T., Maerkl, S. J. and Quake, S. R., 'Microfluidic Large-Scale Integration,' SCIENCE, Vol. 298(2002), pp. 580-584