Effect of Cinnamomi Ramulus Herbal Acupuncture on the Type 1 Hypersensitivity

계지약침(桂枝藥鍼)이 Type 1 Hypersensitivity에 미치는 영향

  • Kim, Dong-Hoon (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dong-Eui University) ;
  • Song, Choon-Ho (Dept. of Meridian & Acupoint, College of Oriental Medicine, Dong-Eui University)
  • 김동훈 (동의대학교 한의과대학 경락경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 경락경혈학교실)
  • Published : 2009.03.27

Abstract

Objectives : We studied anti-allergic effects of Cinnamomi Ramulus(CR) herbal acupuncture and Cinnamomi Ramulus extract. Methods : In vivo, animals were herbal-acupunctured with CR at both ST36s three times for 5 days. Then, we induced active systemic anaphylatic shock using compound 48/80 in ICR mice, and passive cutaneous anaphylaxis using anti-DNP IgE in Sprague Dawley rat. In vitro, we measured cell viability, ${\beta}$ -hexosaminidase release and the expressions of IL-4, TNF-${\alpha}$ and COX-2 mRNA in RBL-2H3 cells after treatment of various concentrations of CR extract. Results : In vivo, CR herbal acupuncture pretreatments at both ST36s inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis was inhibited by CR herbal acupuncture pretreatments at both ST36s and optional points. In vitro, CR extract treatments did not affect on cell viability and inhibited ${\beta}$-hexosaminidase release. CR extract treatments also decreased the expressions of IL-4, TNF-${\alpha}$ and COX-2 mRNA in RBL-2H3 cells. Conclusions : These results suggest that CR herbal acupuncture and CR extract should be beneficial in the inhibition of allergic inflammatory response.

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