Effects of Seonpye-tang on OVA-Induced Asthmatic Mice

천식모델생쥐에서 선폐탕(宣肺湯)의 면역조절 효과

  • Lee, Ju-Guan (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Park, Yang-Chun (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
  • 이주관 (대전대학교 한의과대학 폐계내과학교실) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Published : 2009.04.25


This study aimed to evaluate the anti-asthmatic effects of Seonpye-tang (SPT) using OVA-induced asthmatic mice model. Scavenging activity of SPT on DPPH free radical and SOD-like activity of SPT were measured at final concentration 62.5, 125, 250, 500 (${\mu}g/m{\ell}$), RBL-2H3 cells were treated with DNP IgE for 24hr, and treated with SPT (1, 10, 100 ${\mu}g/m{\ell}$) for 1hr, followed by treatment with DNP-HSA for 1hr at $37^{\circ}C$. The level of IL-4 and TNF-${\alpha}$ were measured by ELISA. Asthmatic mice model was conducted by repeated challenge of OVA using C57BL/6 mice. Each group was treated with distilled water, SPT (400 mg/kg and 200 mg/kg) extract or cyclosporin A (10 mg/kg) for the later 8 weeks. Immune cells subpopulation, eotaxin, IL-5 and TNF-${\alpha}$ in BALF were analyzed. SPT dose-dependently increased Scavenging activity on DPPH free radical and SOD-like activity. SPT significantly ameliorated the increase of total cells number and eosinophil including of immune cell subpopulation of $CD3^+/CD69^+$, $CCR3^+$, $B220^+/CD22^+$, $B220^+/CD45^+$ and $B220^+/IgE^+$ cells in BALF comparing to control group. Eotaxin and IL-5 level in BALF were significantly decreased by SPT. These results strongly suggest that SPT would be a effective candidate for herbal-originated anti-asthmatic drug. However, this drug should be further studied for characterization of the accurate action and underlying mechanisms using variant disease model in the future.


  1. Masoli, M., Fabian, D., Holt, S., Beasley, R. The global burden of asthma: executive summary of the GINA Dissemination Committee report. Allergy 59(5):469-478, 2004 https://doi.org/10.1111/j.1398-9995.2004.00526.x
  2. 국민건강보험공단. 최근 6년(2002~2007)간 황사관련 봄철 다발생 질환 건강보험 의료기관 이용자 분석. http://www.nhic.or.kr.
  3. 전국한의과대학 폐계내과학교실. 동의폐계내과학. 서울, 한문화사, pp 329-331, 2002
  4. 홍천수, 김규언. 기관지천식-병인 및 역학, 대한 천식 및 알레르기학회, 천식과 알레르기 질환. 서울, 군자출판사, pp 244-245, 2002
  5. Holgate, S.T. Pathogenesis of asthma. Clin Exp Allergy 38(6):872-897, 2008 https://doi.org/10.1111/j.1365-2222.2008.02971.x
  6. 허준. 동의보감. 서울, 남산당, p 480, 1986
  7. 송상진, 박양춘. 조각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 26(1):143-155, 2005
  8. 송재진, 박양춘. 반하가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 26(1):156-168, 2005
  9. 정승기, 이형구. 정천탕이 천식에 미치는 영향에 관한 실험적 연구. 경희의학 3(1):91-102, 1987
  10. Bateman, E.D., Boushey, H.A., Bousquet, J., Busse, W.W., Clark, T.J., Pauwels, R.A., Pedersen, S.E. GOAL Investigators Group. Can guideline-defined asthma control be achieved? The Gaining Optimal Asthma ControL study. Am J Respir Crit Care Med. 170(8):836-844, 2004 https://doi.org/10.1164/rccm.200401-033OC
  11. Sch$\ddot{a}$cke, H., D$\ddot{o}$cke, W.D., Asadullah, K. Mechanisms involved in the side effects of glucocorticoids. Pharmacol Ther. 96(1):23-43, 2002 https://doi.org/10.1016/S0163-7258(02)00297-8
  12. Booth, B.W., Newcomb, D.C., McKane, S.A., Crews, A.L., Adler, K.B., Bonner, J.C., Martin, L.D. Proliferation of the airway epithelium in asthma: are inflammatory cells required? Chest. 123(3 Suppl):384S-385S, 2003 https://doi.org/10.1378/chest.123.3_suppl.384S
  13. Hartnel, A., Robinson, D.S., Kay, A.B., Wardlaw, A.J. CD69 is expressed by human eosinophils activated in vivo in asthma and in vitro by cytokines. Immunology. 80(2):281-286, 1993
  14. Tedder, T.F., Tuscano, J., Sato, S., Kehrl, J.H. CD22, a B lymphocyte-specific adhesion molecule that regulates antigen receptor signaling. Annu Rev Immunol. 15: 481-504, 1997 https://doi.org/10.1146/annurev.immunol.15.1.481
  15. 조상헌. IgE 항체 생성과 조절. 알레르기, 13: 460-464, 1993
  16. 이영우, 정희재, 이형구, 정승기. 정천탕과 청상보하탕이 asthma model 내의 cytokine에 미치는 영향. 대한한방내과학회지 22(3):367-377, 2001
  17. Sabroe, I., Conroy, D.M., Gerard, N.P., Li, Y., Collins, P.D., Post, T.W., Jose, P.J., Williams, T.J., Gerard, C.J., Ponath, P.D. Cloning and characterization of the guinea pig eosinophil eotaxin receptor, C-C chemokine receptor-3: blockade using a monoclonal antibody in vivo. J Immunol. 161(11):6139-6947, 1998
  18. Combadiere, C., Ahuja, S.K., Murphy, P.M. Cloning and functional expression of a human eosinophil CC chemokine receptor.J Biol Chem. 270(28):16491-16494, 1995 https://doi.org/10.1074/jbc.270.28.16491
  19. Gangur, V., Birmingham, N.P., Thanesvorakul, S., Joseph, S. CCR3 and CXCR3 as drug targets for allergy. principles and potential. Curr Drug Targets Inflamm Allergy. 2(1):53-62, 2004 https://doi.org/10.2174/1568010033344453
  20. Adcock, I.M., Caramori, G., Chung, K.F. New targets for drug development in asthma. Lancet. 372(9643):1073-1087, 2008 https://doi.org/10.1016/S0140-6736(08)61449-X
  21. Brightling, C., Berry, M., Amrani, Y. Targeting TNF-alpha: a novel therapeutic approach for asthma. J Allergy Clin Immunol. 121(1):5-10, 2008 https://doi.org/10.1016/j.jaci.2007.10.028
  22. Rabe, K.F., Adachi, M., Lai, C.K., Soriano, J.B., Vermeire, P.A., Weiss, K.B., Weiss, S.T. Worldwide severity and control of asthma in children and adults: the global asthma insights and reality surveys. J Allergy Clin Immunol. 114(1):40-47, 2004 https://doi.org/10.1016/j.jaci.2004.04.042
  23. Related Articles, LinksKing, M.R., Ismail, A.S., Davis, L.S., Karp, D.R. Oxidative stress promotes polarization of human T cell differentiation toward a T helper 2 phenotype. J Immunol. 176(5):2765-2772, 2006 https://doi.org/10.4049/jimmunol.176.5.2765
  24. Gerberick, G.F., Cruse, L.W., Ryan, C.A., Hulette, B.C., Chaney, J.G., Skinner, R.A., Dearman, R.J., Kimber, I. Use of a B cell marker (B220) to discriminate between allergens and irritants in the local lymph node assay. Toxicol Sci. 68(2):420-428, 2002. https://doi.org/10.1093/toxsci/68.2.420
  25. 전국한의과대학 본초학교실. 本草學. 서울, 영림사, p 295, 370, 409, 506, 509, 1994
  26. 한영주, 박양춘. 감초(Glycyrrhiza uralensis Fisch, GLU)가 천식모델 생쥐의 BALF내 면역세포 및 Cytokine에 미치는 영향. 대한한방내과학회지 25(3):408-417, 2004
  27. 곽상교, 최선미, 박양춘. 관동화가 천식모델 생쥐의 BALF내 면역세포 및 사이토카인에 미치는 영향. 동의생리병리학회지 19(3):716-721, 2005
  28. Forssmann, U., Uguccioni, M., Loetscher, P., Dahinden, C.A., Langen, H., Thelen, M., Baggiolini, M. Eotaxin-2, a novel CC chemokine that is selective for the chemokine receptor CCR3, and acts like eotaxin on human eosinophil and basophil leukocytes. J Exp Med 185(12):2171-2176, 1997 https://doi.org/10.1084/jem.185.12.2171
  29. 이정은, 박양춘. 삼자화담전이 천식모델 생쥐의 면역세포인에 미치는 영향. 대한한방내과학회지 29(3):742-751, 2008
  30. 임지택, 박양춘. 자하거 약침이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 동의생리병리학회지 19(2):408- 417, 2005
  31. Kirkham, P., Rahman, I. Oxidative stress in asthma and COPD: antioxidants as a therapeutic strategy. Pharmacol Ther. 111(2):476-494, 2006 https://doi.org/10.1016/j.pharmthera.2005.10.015
  32. Justement, L.B. The role of the protein tyrosine phosphatase CD45 in regulation of B lymphocyte activation. Int Rev Immunol. 20(6):713-738, 2001 https://doi.org/10.3109/08830180109045587
  33. Chung, K.F., Barnes, P.J. Cytokines in asthma. Thorax. 54(9):825-857, 1999 https://doi.org/10.1136/thx.54.9.825
  34. Dabbagh, K., Takeyama, K., Lee, H.M., Ueki, I.F., Lausier, J.A., Nadel, J.A. IL-4 induces mucin gene expression and goblet cell metaplasia in vitro and in vivo. J Immunol. 162(10):6233-6237, 1999
  35. 나도균, 박양춘. 가미지황탕이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한의학회지 29(2):7-20, 2008
  36. Son, C.G., Seol, I.C. The experimental study of Jungchun-tang on allergies. 대한한의학회지 24(3):65-71, 2003
  37. 조철준, 임도희, 황지호, 양수영, 박양춘. 황금이 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대한한방내과학회지 27(1):114-125, 2006
  38. 염종훈, 정희재, 정승기, 이형구. 정천탕과 정천탕가미방이 알레르기 천식모델 흰쥐의 BALF내 면역세포 및 혈청 IgE에 미치는 영향. 대한한의학회지 24(1):169-180, 2003
  39. Erin, E.M., Williams, T.J., Barnes, P.J., Hansel, T.T. Eotaxin receptor (CCR3) antagonism in asthma and allergic disease. Curr Drug Targets Inflamm Allergy. 1(2):201-214, 2002 https://doi.org/10.2174/1568010023344715