Identification and Cultural Optimization of the Fenitrothion-degrading Microorganism, Bacillus sphaericus NFo1

Fenitrothion 분해미생물 Bacillus sphaericus NFo1의 동정 및 분해 최적조건

  • Choi, Hyuek (Deagu Research Institute of Public Health and Environment) ;
  • Lee, Young-Deuk (Division of Life and Environmental Science, Daegu University) ;
  • Kang, Sun-Chul (Department of Biotechnology, Daegu University)
  • 최혁 (대구광역시 보건환경연구원) ;
  • 이영득 (대구대학교 생명환경학부) ;
  • 강선철 (대구대학교 생명공학과)
  • Published : 2009.03.31

Abstract

A study was carried out to find out the methodology of biological treatment for wastes and wastewater caused by an organophosporus insecticide, fenitrothion, using fenitrothion-degrading microorganism. A fenitrothion-degrading microorganism was isolated by using a selective nutrient broth (NB) medium including fenitrothion, and identified to Bacillus sphaericus NFol based on its morphological and biochemical characteristics. Further, investigation was processed to determine the optimal culture conditions degrading fenitrothion in NB medium by using the NFo1 strain. As results, the cultural conditions determined for temperature, initial pH and inoculum for the optimum growth of the strain and degradation of fenitrothion, which has a exact co-relationship between both of them, were $35^{\circ}C$, 7.5 and 1.5 at $OD_{660}$ value, respectively. In this conditions, fenitrothion could be degraded within 5 days over 90% at the high concentrations of fenitrothion, upto 200 mg/L.

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