Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum

세균성 시들음병에 대한 식물성 유산균(Lactobacillus sp.)의 저해효과

  • Shrestha, Anupama (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Choi, Kyu-Up (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Lim, Chun-Keun (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University) ;
  • Hur, Jang-Hyun (Division of Biological Environment, College of Agricultural and Life Sciences, Kangwon National University) ;
  • Cho, Sae-Youll (Division of Applied Biology, College of Agriculture and Life Science, Kangwon National University)
  • 아누파마슈레스타 (강원대학교 생물자원공학부 응용생물) ;
  • 최규업 (강원대학교 생물자원공학부 응용생물) ;
  • 임춘근 (강원대학교 생물자원공학부 응용생물) ;
  • 허장현 (강원대학교 자원생물환경학과) ;
  • 조세열 (강원대학교 생물자원공학부 응용생물)
  • Published : 2009.03.31

Abstract

An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 $(4{\times}10^8 cfu/ml)$ into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.

References

  1. Altschul, S.F., W. Gish, W.E. Miller, W. Myers and D. J. Lipman (1990) Basic local alignment search tool. J. Mol. Bioi. 215:403-410
  2. Benkerroum, N. and W. E Sandine (1988) Inhibitory action of nisin against Listeria monocytogenes. J. Dairy Sci. 71 :3237-3245 https://doi.org/10.3168/jds.S0022-0302(88)79929-4
  3. Bernal, G., A. Illanes and L. Ciampi (2002) Isolation and partial purification of a metabolite from a mutant strain of Bacillus sp. with antibiotic activity against plant pathogenic agents. E.J.B. ISSN:0717-3458
  4. Chen, Y.-S., F. Yanagida and T. Shinohara (2005) Isolation and identification of lactic acid bacteria from soil using an enrichment procedure. Lett in Appl Microbiol. 40: 195-200 https://doi.org/10.1111/j.1472-765X.2005.01653.x
  5. Dalal, N. R., S. R. Dalal, V. G. Golliwar and R. I. Khobragade (1999) Studies on grading and pre-packaging of some bacterial wilt resistant brinjal (Solanum melongena L.) varieties. J. Soils Crops. 9:223-226
  6. Dong, C., X. Zeng and Q. Liu (1999) Biological control of tomato bacterial wilt with avirulent bacteriocinogenic strain of Ralstonia solanacearum. J. S. China Agric Univ. 20: 1-4
  7. EI Abdyal, M. S., M.A. el Sayed and A. R. el Shanshourie (1996) Effects of culture conditions on the antimicrobial activities of UV-mutants of Streptomyces corchorusii and S. spiroverticillatus against bean and banana wilt pathogens. Microbiol. Res. 151:201-211 https://doi.org/10.1016/S0944-5013(96)80045-0
  8. Hamad S.H., M.C. Dieng, M.A. Ehrmann and R.F. Vogel (1997) Characterization of the bacterial flora of sudanese sorghum flour and sorghum sourdough. J. Appl. Microbiol. 83:764-770 https://doi.org/10.1046/j.1365-2672.1997.00310.x
  9. Hammes, W.P., N. Weiss, and W.P. Holzapfel (1991) The genera Lactobacillus and Carnobcterium. In the prokaryotes. A handbook on the biology of bacteria: ecoophysiology, isolation, identification, applications (eds Balwos, A., Truper, H. G., Dworkin, M., Harder, W. and Schleifer, K.H. Springer, New york, USA, pp, 1535-1594
  10. Han K.S., Y. Kim, S.H. Kim and S. OH (2007) Characterization and purification of acidocin 1B, a bacteriocin produced by Lactobacillus acidophilus GP1B. J. Microbiol. Biotechnol. 5:774-83
  11. Hayward, A. C. (1991) Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29:65-87 https://doi.org/10.1146/annurev.py.29.090191.000433
  12. Jungi. Ki Suk, Nagesh. S. Ipper, Seon Hwa Lee, Anupama Shrestha, Hye Jin You, Joon Hee Han, Jun Mo Cho and Chun Keun Lim (2007) Effects of a soil-born Paenibacillus sp. strain KPB3 on suppression of bacterial wilt disease caused by Ralstonia solanacearum. The Korean J. of Pesticide Science. 10:313-319
  13. Kang, Y., G. Mao, C. Lu and L. He (1995) Biological control of bacterial wilt of tomato by extracellular protein defective mutants of Pseudomonas solanacearum. Acta Phytophylacica Sin. 22:287-288
  14. Katayama. K and Kimura. S (1987) Ecology and protection of bacterial wilt of potato 2. Some control methods and their integration. Bull. Nagasaki Agric. For. Exp. Stn. 15:29-57
  15. Lemessa, F and W. Zellar (2007) Screening rhizobacteria for biological control of Ralstonia solanacearum in Ethiopia. Bio control. 42:336-344 https://doi.org/10.1016/j.biocontrol.2007.05.014
  16. Laitila, A., H.L. Alakomi, L. Raaska, T. Mattila-Sandholm and A. Haikara (2002) Antifungal activities of two Lactobacillus plantarum strains against fusarium moulds in vitro and in malting of barley. J. Appl. Microbiol. 93:556-576
  17. Molina, L., C. Ramos, M.C. Roncheil, S. Molin and J.L. Ramos (1998) Construction assays. Appl. Environ. Microbiol. 64:2072-2078
  18. Mundt, J. O., and J. L. Hammer. (1968) Lactobacilli on plants. Appl. Microbiol. 16: 1326-1330
  19. Ouwehand, A.C. (1998) Antimicrobial components from lactic acid bacteria. in lactic acid bacteria microbiology and functional aspects ed. Salminen, S. and von Wright, A. pp.New York:Marcel Dekker. pp. 139-159
  20. Perry, S.F. (1995) Freeze drying and cryopreservation of bacteria. In: methods in molecular biology. J. G. Day and M. R. McLellan (eds.) Humana Press Inc. 21-30
  21. Piard J.C. and M. Lait Desmazeaud (1991) Inhibiting factors produced by lactic acid bacteria: oxygen metabolites and end-products from catabolism. 71:525-541
  22. Ronel Visser, H. Wilhelm Holzapfel, J. Johannes, T. Bezuidenhout, and M Kotze Johann es (1986) Antagonism of lactic acid bacteria against phytopathogenic bacteria. Appl. Environ. 52:552-555
  23. Sambrook, J and D.W. Rusell (2001) Molecular Cloning: A laboratory manual. Cold harbour laboratory press, Cold Spring Harbor, New York, USA
  24. Seuk Hyun Ko and Ahn Cheol (2000) Bacteriocin production by Lactococcus lactis KCA2386 isolated from white kimchi. The Korean Journal of Food Science and Technology. 9:263-269
  25. Sharpe, M E. (1981) The genus Lactobacillus. p. In M. P. Starr. H. Stolp. H. G. Trulper. A. Barlows. and H. G. Schlegel. The prokaryotes. Springer-Verlag, Berlin. pp. 1653-1679
  26. Stiles, E.M. (1996) Biopreservation by lactic acid bacteria. AntonieLeeuwenhoek. 70:331-345
  27. Stirling, A. C., and R. Whittenbury (1963) Sources of the lactic acid bacteria occurring in silage. J. Appl. Bacterial. 26:86-90 https://doi.org/10.1111/j.1365-2672.1963.tb01160.x
  28. Sung, P. J., J. K. Shin, H. B. Cho, and S. D. Kim (2005) Isolation, identification and biological control activity of SKU-78 strain against Ralstonia solanacearum. J. Korean Soc. Appi. Biol. Chem. 48:48-52
  29. Suzuki, T. and K. Yamasato (1994) Phylogeny of spore-forming LAB based on 16S rRNA gene sequences. FEMS Microbiol Lett. 115:13-17 https://doi.org/10.1111/j.1574-6968.1994.tb06607.x
  30. Vincent, V. M. and T. W. Mew (1998) Effect of a soil amendment on the survival of Ralstonia solanacearum in different soils. Phytopathology. 88:300-305 https://doi.org/10.1094/PHYTO.1998.88.4.300
  31. Weisburg, W. G., S. M. Barns, D. A. Pelletier and D. J. Lane (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol. 173:697-703
  32. Wen-Hsin Lin., Hwang Chin-Fa, Chen Li-Wei, Tsen Hau-Yang (2006) Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23:74-81 https://doi.org/10.1016/j.fm.2005.01.013
  33. Wood, B. J. B. and W.H. eds. Holzapfel (1955) The genera of lactic acid bacteria. Blackie Academic & Professional, UK