Inhibitory Effect of Garlic on the Growth of Aspergillus parasiticus

한국산 마늘에 의한 Aspergillus parasiticus의 성장 억제 효과

  • 박정영 (계명대학교 공중보건학과) ;
  • 김종규 (계명대학교 공중보건학과)
  • Published : 2009.04.30


This study was performed to investigate the possible effect of garlic produced in Korea on the inhibition/reduction of growth of A. parasiticus, a toxigenic strain. The effect was studied using different concentrations of freeze-dried garlic in potato-dextrose agar (PDA) and/or in yeast-extract sucrose (YES) broth at $25^{\circ}C$ for 15 days. While inhibition of the fungal growth due to increasing the concentration of garlic was observed, the more remarkable effect was observed on the ninth day. Reduction of fungal diameter as a result of addition of garlic on PDA was observed to range between 3.4% to 20.1 % while reduction of mycelial weight in YES broth ranged from 9.9% to 30.5%. The 0.5% and 1.0% concentrations of garlic significantly reduced fungal diameter in PDA on the 9th day, while 0.1 %, 0.5%, and 1.0% concentrations of garlic significantly reduced the mycelial weight in YES broth (p<0.05). Dose-response relationships were observed between the concentration of garlic and inhibition of growth both in solid culture and in liquid culture. This study indicates that garlic could be an effective inhibitor at a human consumption level of the growth of A. parasiticus. More research is needed to study the inhibitory effects of the main active component of garlic.


  1. Mishra, H. N. and Das, C. : A review on biological control and metabolism of aflatoxin. Critical Reviews in Food Science and Nutrition, 43, 245-264, 2003
  2. Smith, J. E. and Moss, M. O. : Mycotoxins, formation, analysis and significance, John Wiley & Sons, New York, 60-65, 1985
  3. Kim, J. G. : Food hygiene and sanitation, Sihinkwang Publishing Co., Seoul, 210-214, 2006 (Korean)
  4. Kim, J. G. and Lee, Y. W. : Grain development and aflatoxin B 1 accumulation in preharvest rice inoculated with Aspergillus parasicitus. Journal of Food Protection 59(12), 1318-1321, 1996
  5. Kim, J. G. : Occurrence of aflatoxins in rice and rice products and by-products of rice: a review. Korean Journal of Environmental Health, 32(5), 515-521, 2006
  6. Wilson, C. L. and Droby, S. : Microbial food contamination, CRC Press, New York, 151-154, 2001
  7. Bachmann, F. M. : The inhibiting action of certain spices on the growth of microorganism. The Journal of Industrial and Engineering Chemistry, 7(8), 620-623, 1916
  8. Cavallito, C. J., Buck, J. S. and Suter, C. M. : Allicin, the antibacterial principle of Allium sativum, II. Determination of the chemical structure. Journal of the American Chemical Society, 66, 1952-1954, 1944
  9. Jeong, D. H. and Jeong, S. W. : The science of garlic, World Science, Seoul, 52, 2005 (Korean)
  10. Sheo, H. J. : The antibacterial action of garlic, onion, and red pepper juice. Journal of Korean Society of Food Science and Nutrition, 28(1), 94-99, 1999(Korean)
  11. Kim, J. G. and Lee, K. M. : Inhibitory effect of Korean fermented vegetable (kimchi) on the growth and aflatoxin production of Aspergillus parasicitus Part 1. Journal of Environmental Health Sciences 33(3), 190-194, 2007
  12. Kim, J. G., Lee, Y. W., Kim, P. G., Roh, W. S. and Shintani, H. : Reduction of aflatoxins by Korean soybean paste and its effect on cytotoxicity and reproductive toxicity-part 1. Inhibition of growth and aflatoxin production of Aspergillus parasiticus by Korean soybean paste (doen-jang) and identification of the active component. Journal of Food Protection, 63, 1295-1298, 2000
  13. Buchanan, R. L. and Ayres, J. C. : Effect of initial pH on aflatoxin production. Applied Microbiology, 30(6), 1050-1051, 1975
  14. Gowda, N. K. S., Malathi, V. and Suganthi, R. U. : Effect of some chemical and herbal compounds on growth of Aspergillus parasiticus and aflatoxin production. Animal Feed Science and Technology, 116, 281-291, 2004
  15. Graham, H. D. and Graham, E. J. F. : Inhibition of Aspergillus parasiticus growth and toxin production by garlic. Journal of Food Safety, 8(2), 101-108, 1987
  16. Woo, Y. S. and Chung, D. H. : The effects of garlic (Allium sativum L.) extract on growth, lipid and aflatoxin production by Aspergillus parasiticus R-716. Korean Journal of Environmental Health, 10(2), 89- 97, 1984 (Korean)
  17. Garcia, R. P. and Garcia, M. I. : Laboratory evaluation of plant extracts for the control of Aspergillus growth and aflatoxin formation. Proceedings of the Japanese Association of Mycotoxicology Supplement, 1, 190-193, 1988
  18. Yamata, Y. and Azuma, K. : Evaluation of the in vitro antifungal activity of allicin. Antimicrobial Agents and Chemotherapy 11(4), 743-749, 1977
  19. Korea Ministry of Health and WelfarelKorea Health Industry Development Institute: The Third national Health & Nutrition Examination Survey, 123-134, 2005
  20. Kim, Y. S., Seo, H. Y., No, K. M., Shim, S. L., Yang, S. H., Park, E. R. and Kim, K. S. : Comparison of volatile organic components in fresh and freeze dried garlic. Journal of Korean Society of Food Science and Nutrition, 34(6), 885-891, 2005 (Korean)

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