Antimicrobial Studies of Stem of Different Berberis Species

  • Singh, Meenakshi (Pharmacognosy & Ethnopharmacology Division, National Botanical Research Institute) ;
  • Srivastava, Sharad (Pharmacognosy & Ethnopharmacology Division, National Botanical Research Institute) ;
  • Rawat, Aks (Pharmacognosy & Ethnopharmacology Division, National Botanical Research Institute)
  • Published : 2009.06.30

Abstract

Berberis is an important medicinal plant, of the family Berberidaceae. Different Berberis species and their parts are very common in herbal drug markets of India and world over as an adulterant/substitute to 'Daruharidra' i.e. B. aristata DC. Antimicrobial activity of 50% hydroalcoholic extracts of stem of four Berberis species viz. B. aristata DC., B. asiatica Roxb. ex DC., B. chitria Lindl. and B. lycium Royle and the isolated alkaloid berberine were tested against eleven bacterial and eight fungal strains. The extracts with the strongest antibacterial activity was obtained from B. lycium followed by B. aristata, B. asiatica and B. chitria. Based on these results it is possible to conclude that the hydroalcoholic extract and alkaloid (berberine) has stronger and broader spectrum against bacterial strains as compared to fungal strains. The result obtained in the present study authenticates and support the use of these plants in folklore medicine for treatment of various infectious diseases caused by the bacterial pathogens. However, an attempt has been made to explore the possibilities of utilizing stem part rather than roots of these species with the aim to conserve this species which is over exploited due to diverse use of its root. These findings will stimulate the search for novel, natural products as new antibacterial/antifungal agents which may be useful to pharmaceutical industries.

References

  1. Anonymous, Indian Pharmacopoeia, 2nd Edn. Govt. of India; New Delhi, 1965
  2. Anonymous, The wealth of India Berberis Linn. (Berberidaceae). Ed Ambastha Sp Publication and Information Directorate, CSIR, New Delhi, India. 2B, pp. 114-118, 1988
  3. Bhandari, D.K., Nath, G., Ray, A.B., and Tewari, P.V., Antimicrobial activity of crude extracts from Berberis asiatica stem bark. Pharm. Biol., 38, 254-257 (2000) https://doi.org/10.1076/1388-0209(200009)38:4;1-A;FT254
  4. Chopra, R.N., Chopra, T.C., Handa, K.L., and Kapoor, L.D., Chopra’s Indigenous Drugs of India, 284. U.N. Dhar and Sons Pvt. Ltd., 1958
  5. Dutta, N.K. and Panse, M.V., Usefulness of berberine (an alkaloid from Berberis aristata) in the treatment of cholera. Indian J. Med. Res., 50, 732-735 (1962)
  6. Gorsvenor, P.W., Supriono, A., and Gray, D.O., Medicinal plants from Riau Provinces, Sumatra, Indonesia. Part-2. Antibacterial and Antifungal activity. J. Ethnopharmacol., 45, 97-111 (1995) https://doi.org/10.1016/0378-8741(94)01210-Q
  7. Kirtikar, K.R. and Basu, B.D., Indian Medicinal Plants- I, Lalit Mohan Basu and Co., Allahabad, 1933
  8. Lahiri, S.C. and Dutta, N.K., Berberine and chloramphenicol in the treatment of cholera and severe diarrhoea. J. Ind. Med. Assoc., 48, 1- 11 (1967)
  9. Murray, P.R., Baron, E.J., Pfallar, M.A., Tenover, F.C., and Yalk, R.H., Mannual of clinical microbiology. 6th ed. ASM, Washington D.C., 1995
  10. National committee for clinical Laboratory standards. Reference method broth dilution Antifungal Susceptibility Testing of Yeast. Tentative Standards M 27-T, National committee for clinical Laboratory Standards, Villanova, PA., 1995
  11. National committee for clinical Laboratory Standards. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th ed. Approved standards M7-A5, Wayne, PA: NCCLS., 2000
  12. Siwon, J., Verpoorte, R., Van Essen, G.F.A., Baerhem, A., and Vendson, S., Studies on Indonesia medicinal plants. Planta Med., 38, 24-32 (1980) https://doi.org/10.1055/s-2008-1074833
  13. Srivastava, S., Rawat, A.K.S., Srivastava, M., and Mehrotra, S., Pharmacognostic evaluation of roots of Berberis chitria Lindl, Nat. Prod. Sci., 12, 19-23 (2006)
  14. Srivastava, S.K., Khatoon, S., Rawat, A.K.S., Mehrotra, S., and Pushpangadan, P., Pharmacognostical studies of the roots of Berberis aristata DC. Nat. Prod. Sci., 7, 102-106 (2001)
  15. Srivastava, S.K., Rawat, A.K.S., and Mehrotra, S., Pharmacognostic Evaluation of the Root of Berberis asiatica Roxb. ex DC. Pharm. Biol., 42, 467-473 (2004) https://doi.org/10.1080/13880200490886256
  16. Sushrut Samhita, Sutrasthanam Lakshadi Group. Ed Bhishagratna KK, Chaukhamba Sanskrit Sansthan, Varanasi, India. 1, pp. 354, 1963
  17. Watt, G., Economic products of India V, The superintendent of Government Printing, India 1883