Optimization of Extraction Conditions of Polyphenolic Compounds from Apple Pomace by Response Surface Methodology

반응표면분석에 의한 사과 pomace로부터의 폴리페놀 추출조건 최적화

  • Published : 2009.06.30

Abstract

This study examined the optimization of alcohol extraction conditions for maximizing the total polyphenols derived from apple pomace, by response surface methodology (RSM). The effects of four independent variables, including $X_1$ (ratio of solvent to sample content), $X_2$ (dipping time), $X_3$ (extraction time), and $X_4$(extraction temperature), were investigated at five levels using central composite design (CCD). $Y_1$ (yield) and $Y_2$ (total polyphenols) were chosen as dependent variables. The coefficients of determination, $R^2$, were greater than 0.900 (0.9042 and 0.9555). The results showed that the model fit was very significant (p<0.001). The optimum extraction conditions were as follows: 13.00 mL/g for the ratio of solvent to sample content, 89.02 min for dipping time, 180 min for extraction time, and $70^{\circ}C$ for extraction temperature. At these conditions, the predicted total polyphenol content was 29.68 mg catechin equiv./g.

Keywords

apple pomace;total polyphenols;response surface methodology

References

  1. Whang HJ. Changes of phenolic compounds in Korean apple (fuji) during maturation. Korean J. Food Nutr. 12: 364-369 (1999)
  2. Yun HJ, Lim SY, Hur JM, Jeong JW, Yang SH, Kim DH. Changes of functional compounds in, and testure characteristics of, apples, during post-irradiation storage at different temperatures. Korean J. Food Preserv. 14: 239-246 (2007)
  3. Kim SD, Jang KS, Kim MK. Fermentation of apple vinegar in the farmhouse. J. East Asian Soc. Diet. Life 4: 79-90 (1994)
  4. Lu Y, Foo LY. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem. 68: 81-85 (2000) https://doi.org/10.1016/S0308-8146(99)00167-3
  5. Cha JY, Kim HJ, Chung CH, Cho YS. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Korean Soc. Food Sci. Nutr. 28: 1310-1315 (1999)
  6. Hong JJ, Ahn TH. Changes in total flavonoid and total polyphenol contents of leafy vegetables (spinach, chard and whorled mallow) by blanching time. Korean J. Food Cook. Sci. 21: 190-194 (2005)
  7. Kim HK, Do JR, Hong JH, Lee GD. Optimization of extraction conditions for cabbage. J. Korean Soc. Food Sci. Nutr. 34: 1625-1632 (2005) https://doi.org/10.3746/jkfn.2005.34.10.1625
  8. Kim JO, Kwon ST, Lee GD, Hong JH, Moon DH, Kim TW, Kim DI. Optimization of extraction condition on fig (Ficus carica L.) by response surface methodology. Korean J. Food Preserv. 15: 66-73 (2008)
  9. Singleton V, Rossi J. Colorimetry of total phenolics with phosphomolybdic- phosphotungstic acid reagents. Am. J. Enol. Viticult. 16: 144-158 (1965)
  10. An BJ, Park TS, Lee JY, Park GH, Hyun SJ, Lee JT, Cho YJ, Kim YS. Growth inhibitory effect of irradiated green tea polyphenol addition in cosmetic composition. J. Korean Soc. Appl. Biol. Chem. 50: 217-223 (2007)
  11. Lu Y, Foo LY. Identification and quantification of major polyphenols in apple pomace. Food Chem. 59: 187-194 (1997) https://doi.org/10.1016/S0308-8146(96)00287-7
  12. Lee MS, Ryu GH. Optimization of hot water extraction conditions for cod byproduct by response surface methodology analysis. Food Eng. Prog. 10: 248-255 (2006)
  13. Adil IH, Cetin HI, Yener ME, Bayindirli A. Subscritical (carbon dioxide+ethanol) extraction of polyphenols from apple and peach pomaces, and determination of the antioxidant activities of the extracts. J. Supercrit. Fluid. 43: 55-63 (2007) https://doi.org/10.1016/j.supflu.2007.04.012
  14. Agricultural & Forestry Statistical Yearbook, Ministry of Agriculture & Forestry, Seoul, Korea. pp. 116-117 (2006)
  15. Abad-Garcia B, Berrueta LA, Lopez-Marquez DM, Crespo-Ferrer I, Gallo B, Vicente F. Optimization and validation of a methodology based on solvent extraction and liquid chromatography for the simultaneous determination of several polyphenolic families in fruit juices. J. Chromatogr. 1154: 87-96 (2007) https://doi.org/10.1016/j.chroma.2007.03.023
  16. Sudha ML, Baskaran V, Leelavathi K. Apple pomace as a source of dietary fiber and polyphenols and its effect on the rheological characteristics and cake making. Food Chem. 104: 686-692 (2007) https://doi.org/10.1016/j.foodchem.2006.12.016
  17. Sohn KS, Seog EJ, Lee JH. Quality characteristics of clarified apple juices produced by various methods. Korean J. Food Preserv. 13: 138-143 (2006)
  18. Whang HJ, Han WS, Yoon KR. Quantitative analysis of total phenolic content in apple. Anal. Sci. Technol. 14: 377-383 (2001)
  19. Renard CMGC, Dupont N, Guillermin P. Concentrations and characteristics of procyanidins and other phenolics in apples during fruit growth. Phytochemistry 68: 1128-1138 (2007) https://doi.org/10.1016/j.phytochem.2007.02.012
  20. Kim NM, Sung HS, Kim WJ. Effect of solvents and some extraction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol. 25: 204-209 (1993)
  21. Jang DH. Maple Use for RSM education. Korea Satistical Society, Seoul, Korea (2005)
  22. Kim HK, Do JR, Hong JH, Lee GD. Optimization for functional properties of cabbage extracts. Korean J. Food Preserv. 12: 591-599 (2005)
  23. Choi MA, Park NY, Woo SM, Jeong YJ. Optimization of extraction conditions from Hericium erinaceus by response surface methodology. Korean J. Food Sci. Technol. 35: 777-782 (2003)
  24. Park NY, Kwon JH, Kim HK. Optimization of extraction conditions for ethanol extracts from chrysanthemum morifolium by response surface methodology. Korean J. Food Sci. Technol. 30: 1189-1196 (1998)