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In vitro Anti-proliferative Characteristics of Flavonoids and Diazepam on MDA-MB-231 Breast Cancer Cells

Flavonoid류와 diazepam의 시험관 내 MDA-MB-231 유방암세포 증식 억제 효과

  • Kim, Ji-Kwan (Department of Pharmacology, School of Medicine, Kyungpook National University) ;
  • Lee, Maan-Gee (Department of Pharmacology and 1Nuclear Medicine, School of Medicine, Kyungpook National University) ;
  • Lee, Jae-Tae (Department of Nuclear Medicine, School of Medicine, Kyungpook National University) ;
  • Ha, Jeoung-Hee (Department of Pharmacology, School of Medicine, Kyungpook National University)
  • 김지관 (경북대학교 의학전문대학원 약리학교실) ;
  • 이만기 (경북대학교 의학전문대학원 약리학교실) ;
  • 이재태 (경북대학교 의학전문대학원 핵의학교실) ;
  • 하정희 (경북대학교 의학전문대학원 약리학교실)
  • Published : 2009.08.30

Abstract

The beneficial use of sedatives is often required for medically ill patients. This study examined the effect of plant flavonoids and diazepam peripheral-type benzodiazepine receptor (PBR) activation and glucose utilization in breast cancer cells, along with their interactions. In estrogen receptor negative MDA-MB-231 cells, the anti-proliferative activity of fisetin (3,7,3',4'-tetrahydroxyflavone) and diazepam was more prominent than in estrogen receptor positive MCF-7 cells. Unlike PBR ligands, treatment with $10^{-6}$ M concentration of diazepam for 3 days exhibited anti-proliferative effects, while similar to apigenin (4',5,7-Trihydroxyflavone) and fisetin, diazepam hardly affected the PBR mRNA expression by MDA-MB-231 cells. Treatment with $10^{-6}$ M concentration of flavonoids and diazepam for 3 days inhibited the glucose utilization of MDA-MB-231 cells. Treatment with $10^{-6}$ M concentration of flavonoids and diazepam for 6 days showed increased cytotoxicity and reduced the PBR mRNA expression of the MDA-MB-231 cells. Apigenin enhanced diazepam-induced anti-proliferative effects on the MDA-MB-231 cells as well. All together, this study showed the in vitro anti-proliferative activity of flavonoids and diazepam on MDA-MB-231 breast cancer cells, plus additive enhancements. In conclusion, this study provides experimental basis for advanced trials in the future.

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