Scutellaria baicalensis Georgi(SBG) inhibits Melanin Synthesis in Mouse B16 Melanoma Cells

α-MSH 유도성 멜라닌 합성에 있어서 황금 추출물의 역할과 작용기전 연구

  • 홍성진 (경원대학교 한의과대학 안이비인후피부과학교실) ;
  • 김경준 (경원대학교 한의과대학 안이비인후피부과학교실)
  • Published : 2009.08.25


Objective : Melanin is one of the most important facor in skin color. Melanin protects human skin from ultraviolet radiation otherwise it causes melanin pigmentation. So this experiment is carried out for test whether Scutellaria baicalensis Georgi(SBG) inhibits melanin synthesis and tyrosinase activity in mouse B16 melanoma cells. Method : The melanin synthesis inhibition effects of SBG were examined by in vitro melanin production assay. We assessed inhibitory effects of SBG on melanin contents from B16F1 melanoma cell, on tyrosinase activity(cell and cell free system), effect of SBG on the expression tyrosinase, Microphthalmia-associated Transcription Factor(MITF), Extracellular signal-regulated Kinase(ERK). Result : SBG inhibited melanin synthesis induced $\alpha$-MSH($\alpha$-Melanin Stimulating Hormone) in B16F1. SBG inhibited tyrosinase activity and expression. And SBG down-regulates MITF and stimulated ERK activation in B16F1. Conclusion : According to above results, SBG was improved its suppression effect to the inhibition of melanin synthesis, tyrosinase activation, and tyrosinase promotor activation. So SBG is considered to be used for an strong source of skin whitening effect.


  1. Maeda K, Fukuda M., In vitro effectiveness of several whitening cosmetic components in human melanocyte, J. Soc. Cosmet Chem, 1991:42,361-8.
  2. Mishima Y., 외. Overview for development of furture innovative skin whitening agents, Fragrance J. 1996;24:13.
  3. Gordon P. R., Mansur C. P., Gilchrest B. A., Regilation of human growth, dendricity and melanization by keratinocyte derived factor, J. Invest, Dermatol, 1989:92,565-72.
  4. Ilza V., Huang Ti Nei Ching Su Wen, Univ.California Press, 1966.
  5. T, Ikeda and T. Tsutsumi, Fuction and skin depigmental activity of crude drugs , Fragrance J.,1990:6, 56.
  6. 김지영, 이연희, 김주연, 노보경, 익모초 추출물의 항산화 작용에 관한 연구. J. soc. Cosmet. Scientists korea, 2005 Jun;31(2):189-96.
  7. 신민교, 원색임상본초학. 서울:남산당. 1986:308-9.
  8. 新文豊出版公社. 中藥大辭典. 臺北:新文豊出版公社. 1981:158,175-9.
  9. Antioxiductive and anti-inflammatory activities of polyhydroxyflavonoids of Scutellaria baicalensis Georgi., Biosci Biotechnol biochem. 2006 Oct;70(10):2371-80.
  10. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complement Altern Med. 2006 Aug;16(6):27.
  11. Inhibition of chronic skin inflammation by topical anti-inflammatory flavonoid preparation. Ato Formula, Arch Pharm Res. 2006 Jun;29(6):503-7
  12. Park, S. H., Kim, D. S., Kim, W. G., Ryoo, I. J., Lee, D. H., Huh, C. H., Youn, S. W., Yoo, I. D., and Park, K. C., A New Melanogenesis Inhibitor and its Mechanism, Cell Mol Life Sci. 2004;6:2878-85.
  13. Busca R., Bertolotto C., Ortonne J. P., Ballotti R., Inhibition of the phosphatidylinositol 3-kinase/p70(S6)-kinase pathway induces B16 melanoma cell differentiation. J. Biol. Chem., 1996;271:31824-30.
  14. Sato, K., Takahashi, H., Iraha, R., and Toriyama, M., Down-regulation of tyrosinase expression by acetylsalicylic acid in murine B16 melanoma. Biol Pharm Bull. 2008;31:33-7.
  15. Busca, R., and Ballotti, R., Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigment Cell Res. 2008;13:60-9.
  16. Michael H. R., Edward J. R., Histology. A text and Atlas. Haper and Row, Lippincott. 1985:338-48.
  17. Elias P. M., Menon G. K., Structural and lipid biochemical correlates of the epidermal permeability barrier, In: Elias P. M., Havel R. J. and Small D. M., eds., Advances in Lipid Research, New York:Skin Lipids. Academic Press Inc.,1991:1-3.
  18. Jimbow K., Fitzpatrick T. B., Wick M Biochemistry and Physiology of melanin pigmentation, In:Goldsmith, L.A., ed., Physiology and Molecular Biology of the skin, New York:Oxford Univ. press. 1991:873-909.
  19. Jimbow K, Quevedo WC, Fitzpatrick TB, et al. Biology of melanocyte. In:Fitzpatrick TB, Eisen AZ, Wolff K, et al, eds. Dermatology in general medicine, 4th ed. New York:McGraw-Kill Book. 1993:261-84.
  20. 박경아 外. 조직학. 서울:고려의학. 1999:405-11.
  21. Bloom W, Fawcett DW:A textbook of histology, 11th ed. USA:W.B. Saunders Company. 1986:543-58.
  22. 최종원. A study on the antimicrobial activity and in vitro cytotoxicity of uv-b sunscreen and preservative in cosmetic products. 건국대학교 대학원 박사학위논문. 1996.
  23. Mishima, Y., Hatta, S., Ohyama, Y., Induction of melanogenesis suppression cellular pharmacology and mode of differential action, Pigment Cell Res., 1988;1:367.
  24. Matubara, H., Inhibitory effect of Iichen metabolites and their synthetic analogues on malanin biosynthesis in cultured B-16 mouse melanoma cells, Natural Product Sciences., 1998;4:3.
  25. Y. Funasaka, A. K. Chakraborty, M. Komoto, A. Ohashi, and M. Ichihashi. The depigmenting effect of c-tocopherol ferulate on human melanoma cells. British J. Dermatology. 1999;141:20-9.
  26. Prota G., Recent advances in the chemistry og melanogenesis in mammals. J. Invest Dermatol. 1990;75(122):31-6.
  27. Jackson I.J. et al. A second tyrosinaserelated protein, TRP-2, is a melonogenic enzyme termed DOPAchrome tautomerase, EMBO J., 1992;11:519.
  28. Jimbow K. et al. Increased sensitivity of melanocytes to oxidative stress and abnormal expression of tyrosinase-related protein in viltigo. British J. Derma. 2001;144:55.
  29. 하병조. 기능성화장품. 서울:신광출판사. 2001:66-84, 138-40, 143-7.
  30. 김은철 외. 천연물에 포함된 미백성분의 추출 및 미백효과. 한국공업화학학회지. 2005;16(3):348-53.
  31. Dawley R. M., Flurkey W. H., 4-Hexylresocinol aponent inhibitor of mushroom tyrosinase. J. Food. Sci. 1993:58,609-10.
  32. 楊維傑 編. 黃帝內徑 素問. 臺北:樂群出版事業有限公司. 1994:624-79.
  33. 陳昭遇 外. 太平聖惠方. 北京:人民衛生出版社. 1986:1208.
  34. 許俊. 東醫寶鑑. 서울:南山堂. 1981:211-2.
  35. 趙佶. 聖濟總錄. 北京:人民衛生出版社. 1987:1763.
  36. 辛民敎 外. 鄕藥集成方. 서울:영림사. 1989:1039.
  37. 周命新. 醫門寶鑑. 서울:杏林書院. 1975:186-7.
  38. 陳實功. 外科正宗. 上海:上海科學技術出版社. 1989:290-8.
  39. 顧世澄. 瘍醫大全. 北京:人民衛生出版社. 1987:479,481-2.
  40. 顧伯華 外. 實用中醫外科學. 上海:上海科學技術出版社. 1985:529-30.
  41. 楊思樹 外. 中醫臨床大全. 中國:北京科學技術出版社. 1991:923-4.
  42. 樓英. 醫學綱目. 서울:대성문화사. 1986:1081.
  43. 王肯堂. 證治準繩. 北京:人民衛生出版社. 1991:824.
  44. 巢元方. 巢氏諸病原候論. 서울:대성문화사. 1992:200.
  45. 李梴. 偏註醫學入門. 서울:대성문화사. 1990:29,224.
  46. 朱震亨. 丹溪醫集. 北京:人民衛生出版社. 1993:24.
  47. 이상인. 본초학. 서울:수서원. 1981:505-7.
  48. 中華人民共和國 衛生部 藥典委員會. 中華人民共和國藥典. 北京:人民衛生出版社. 1977:515.
  49. 上海中醫學院. 中華藥學. 香港商:務印書館. 1981:197-220.
  50. 徐國龍 外. 常用中藥名辨. 安微:安微科學技術出版社. 1981:204-5.
  51. 翁雄健. 中藥應用鑒別. 天津:天津科學技術出版社. 1984:34-5.
  52. 이상인, 안덕균. 본초학. 서울:영림사. 1992:178.