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Chemical Components and Physiological Functionalities of Brassica campestris ssp rapa Sprouts

순무 싹의 화학성분과 생리기능성

  • Ha, Jin-Ok (Dept. of Food and Nutrition, Chosun University) ;
  • Ha, Tae-Man (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University) ;
  • Kim, Ah-Ra (Dept. of Food and Nutrition, Chosun University) ;
  • Lee, Myung-Yul (Dept. of Food and Nutrition, Chosun University)
  • 하진옥 (조선대학교 식품영양학과) ;
  • 하태만 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과) ;
  • 김아라 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2009.10.31

Abstract

This study was carried out to investigate physicochemical and functional properties of dried Brassica campestris ssp rapa (BR) sprouts. The proximate compositions of BR sprouts as dry matter basis were 2.35% of moisture content, 22.51% of crude protein, 21.60% of crude lipid, 4.35% of crude ash, and 49.19% of carbohydrate, respectively. The free sugars were identified as glucose and fructose. Analyzing total amino acids, 18 kinds of components were isolated from BR sprouts. The essential amino acid contained in BR sprouts accounted for 47.00% of total amino acid, while the non-essential amino acid accounted for 53.00%. The contents of vitamin A and vitamin E were 0.09 mg% and 3.06 mg%, respectively. Tartaric acid was the major organic acid. Among the minerals in dried BR sprouts, the content of potassium was the highest (882.50 mg%) and those of magnesium and calcium were comparatively high (342.85 mg%, 274.30 mg%). BR sprouts ethanol extract significantly inhibited the HMG-CoA reductase activity in a concentration-dependent manner in vitro. Furthermore, nitrite scavenging ability and DPPH radical scavenging activity of the ethanol extract of BR sprouts were 64.25% and 69.29% at a concentration of 1,000 ${\mu}g$/mL, respectively. These results suggest that BR sprouts possess potential antioxidative capacity and HMG-CoA reductase inhibitory activity.

References

  1. Song MR. 2001. Volatile flavor components of cultivated radish (Raphanus sativus L.) sprout. Korean J Food & Nutr 14: 20-27.
  2. Gopalan C, Rama Sastri BV, Balasubramanian SC. 2004. Nutritive values of indian foods. National Institute of Nutrition, Indian Council of Medical Research, Hyderabad. Indian.
  3. Khalil AW, Zeb A, Mahmmod F, Tariq S, Khattak AB, Shah H. 2007. Comparison of sprout quality characteristics of desi and kabuli type chickpea cultivars (Cicer arietinum L.). LWT 40: 937-945. https://doi.org/10.1016/j.lwt.2006.05.009
  4. Sattar A, Shah A, Zeb A. 1995. Biosynthesis of ascorbic acid in germinating rapeseed cultivars. Plant Food for Human Nutr 47: 63-70. https://doi.org/10.1007/BF01088168
  5. El-Adawy TA. 2002. Nutritional composition and antinutritional factors of chickpears (Cicer arietinum L.) undergoing different cooking methods and germination. Plant Food for Human Nutr 57: 83-97. https://doi.org/10.1023/A:1013189620528
  6. Woo N, Song ES, Kim HJ, Seo MS, Kim AJ. 2007. The comparison of antioxidative activities of sprouts extract. Korean J Food & Nutr 20: 356-362.
  7. Bang MH, Lee DY, Oh YJ, Han MW, Yang HG, Chung HG, Jeong TS, Choi MS, Lee KT, Baek NI. 2009. Isolation and identification of secondary metabolites from the roots of Brassica rapa. J Plant Biotechnol 36: 64-67. https://doi.org/10.5010/JPB.2009.36.1.064
  8. Jung BS, Shin MK. 1990. Hyang Yak Dae Sa Jun. Young Lim Sa, Seoul, Korea. p 574.
  9. Park YK, Kim HM, Park MW, Kim SR, Choi IW. 1999. Physicochemical and functional properties of turnip. J Korean Soc Food Sci Nutr 28: 333-341.
  10. Kim HR, Lee JH, Kim YS, Kim KM. 2007. Chemical characteristics and enzyme activities of ichon ge-geol radish, ganghwa turnip and Korean radish. Korean J Food Sci Technol 39: 255-259.
  11. Kim SK, Choi YH, Seo JH, Lee JW, Kim YS, Ryu SY, Kang JS, Kim YK, Kim SH. 2004. Chemical constituents from the root of Brassica campestris ssp rapa. Kor J Pharmacogn 35: 259-263.
  12. Itoh H, Yoshida R, Mizuno T, Kudo M, Nikuni S, Karki T. 1984. Study on the contents of volatile isothiocyanate of cultivars of Brassica vegetables. Report of the National Food Research Institute 45: 33-41.
  13. Hertog MGL, Hollman PCH, Katan MB. 1992. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem 40: 2379-2383. https://doi.org/10.1021/jf00024a011
  14. Theander O, Aman P, Miksche G, Yasuda S. 1977. Carbohydrates, polyphenols and lignin in seed hulls of different colors from turnip rapeseed. J Agric Food Chem 25: 270-273. https://doi.org/10.1021/jf60210a042
  15. Ju H, Chong C, Mullin W, Bible B. 1982. Volatile isothiocyanates and nitriles from glucosinolates in rutabaga and turnip. J Am Soc Horicultura Sci 107: 1050-1054.
  16. Wilkinson A, Rhodes M, Fenwick R. 1984. Myrosinase activity of crucifer vegetables. J Sci Food Agric 35: 543-552. https://doi.org/10.1002/jsfa.2740350511
  17. Mongeau R, Brassard R. 1993. Enzymatic-gravimetric determination in foods of dietary fiber as sum of insoluble and soluble fiber fractions: summary of collaborative study. J Assoc Off Anal Chem 76: 923-925.
  18. Sones K, Heaney R, Fenwick G. 1984. An estimate of the mean daily intake of glucosinolates from cruciferous vegetables in the UK. J Sci Food Agric 35: 712-720. https://doi.org/10.1002/jsfa.2740350619
  19. Collins J. 1978. Flavor preference of selected food products from vegetables. J Agric Food Chem 26: 1012-1015. https://doi.org/10.1021/jf60219a040
  20. Yamani M. 1993. Fermentation of brined tunrnip roots using Lactobacillus plantarum and Leuconostoc mesenteroides starter cultures. World J Microbiol Biotechnol 9: 176-179. https://doi.org/10.1007/BF00327831
  21. Morita H, Miyamoto T, Mori K, Kataoka K, Izumimoto M. 1990. Isolation and identification of lactic acid bacteria from pickles. J J apan Dairy and Food Sci 39: A183-A193.
  22. Bang MH, Lee DY, Oh YJ, Han MW, Yang HJ, Chung HG, Jeong TS, Lee KT, Choi MS, Baek NI. 2008. Development of biologically active compounds from edible plant sources ⅩⅩⅡ. Isolation of indoles from the roots of Brassica campestris ssp rapa and their hACAT inhibitory activity. J Korean Soc Appl Biol Chem 51: 65-69.
  23. Choi HJ, Han MJ, Baek NI, Kim DH, Jung HG, Kim NJ. 2006. Hepatoprotective effects of Brassica rapa (turnip) on d-galactosamine induced liver injured rats. Kor J Pharmacogn 37: 258-265.
  24. AOAC. 1995. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA.
  25. Gancedo M, Luh BS. 1986. HPLC analysis of organic acid and sugars in tomato juice. J Food Sci 51: 571-573. https://doi.org/10.1111/j.1365-2621.1986.tb13881.x
  26. Korea Food and Drug Association. 2005. Food standards codex. Korean Foods Industry Association, Seoul, Korea. p 367-368, p 383-385.
  27. Waters Associates. 1990. Analysis of amino acid in waters. PICO. TAG system. Young-in Scientific Co. Ltd., Seoul, Korea. p 41-46.
  28. Wungaarden DV. 1967. Modified rapid preparation fatty acid esters from liquid for gas chromatographic analysis. Anal Chem 39: 848-850. https://doi.org/10.1021/ac60251a031
  29. Jung GT, Ju IO, Choi JS, Hong JS. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT (Omija) seed. Korean J Food Sci Technol 32: 928-935.
  30. Kleinsek DA, Ranganathan S, Porter JW. 1977. Purification of 3-hydroxy-3-methylglutaryl-coenzyme A reductase from rat liver. Proc Natl Acad Sci 74: 1401-1435. https://doi.org/10.1073/pnas.74.4.1431
  31. Kato H, Lee IE, Cheyen NV, Kim SB, Hayase F. 1987. Inhibitory of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 5: 1333-1335.
  32. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200. https://doi.org/10.1038/1811199a0
  33. Lee EH, Kim C. 2008. Nutritional changes of buckwheat during germination. Korean J Food Culture 23: 121-129.
  34. Vanderstoep J. 1981. Effect of the nutritive value of legumes. Food Technol 35: 83-91.
  35. Lee JJ, Lee YM, Kim AR, Lee MY. 2009. Physicocehmical composition of broccoli sprouts. Korean J Life Sci 19: 192-197. https://doi.org/10.5352/JLS.2009.19.2.192
  36. Kim IS, Kwon TB, Oh SK. 1988. Study on the chemical change of general composition, fatty acids and minerals of rapeseed during germination. Korean J Food Sci Technol 20: 188-193.
  37. Han JH, Moon HK, Kim JK, Kim JY, Kang WW. 2003. Changes in chemical composition of radish bud (Raphaus sativus L.) during growth stage. Korean J Soc Food Cookery Sci 19: 596-602.
  38. Kim IS, Kwon TB, Oh SK. 1988. Study on the composition change of free sugars and glucosinolates of rapeseed during germination. Korean J Food Sci Technol 20: 194-199.
  39. Kim IS, Han SH, Han KW. 1997. Study on the chemical change of amino acid and vitamin of rapeseed during germination. J Korean Soc Food Sci Nutr 26: 1058-1062.
  40. Kwon EK, Kim YE, Lee CH, Kim HY. 2006. Screening of nine herbs with biological activities on ACE inhibition, HMG-CoA reductase inhibition, and fibrinolysis. Korean J Food Sci Technol 38: 691-698.
  41. Maher VM, Thompson GR. 1990. HMG-CoA reductase inhibitors as lipid-lowering agents: five years experience with lovastatin and an appraisal of simvastatin and pravastin. Q J Med 274: 165-170.
  42. Lee YH, Lee EO, Park SY, Lee HJ, Yoon BS, Kim JH, Kim SH. 2005. Effect of Brassica rapa L. extract and ${\beta}$- sitosterol on hyperlipidemic rats. Korean J Oriental Physiology & Pathology 19: 1528-1533.
  43. Cho JY, Son DM, Kim JM, Seo BS, Yang SY, Bae JH, Heo BG. 2008. Effect of LED as light quality on the germination, growth and physiological activities of broccoli sprouts. J Bio-Environment Control 17: 116-123.
  44. Hwang EJ, Lee SK, Kwon SJ, Park MH, Boo HO. 2006. Antioxidative, antimicrobacterial and cytotoxic activities of Fagopyum esculentum Moench extract in germinated seeds. Kor Medicinal Crop Sci 14: 1-7.

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