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Single and 14-Day Repeated Oral Toxicity Studies of 70% Ethanol Extract of Lindera Obtusiloba Blume Leaves

생강나무(Lindera obtusiloba Blume) 잎 70% 에탄올 추출물의 단회와 14일 반복투여 독성시험의 안전성 평가

  • Hong, Chung-Oui (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • SeoMun, Young (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Koo, Yun-Chang (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Nam, Mi-Hyun (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Hyun-Ah (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Ji-Hoon (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Wang, Zeng (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Yang, Sung-Yong (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Sung-Hee (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • No, Su-Hwan (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Kwang-Won (Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University)
  • 홍충의 (고려대학교 생명과학대학 식품공학부) ;
  • 서문영 (고려대학교 생명과학대학 식품공학부) ;
  • 구윤창 (고려대학교 생명과학대학 식품공학부) ;
  • 남미현 (고려대학교 생명과학대학 식품공학부) ;
  • 이현아 (고려대학교 생명과학대학 식품공학부) ;
  • 김지훈 (고려대학교 생명과학대학 식품공학부) ;
  • 왕증 (고려대학교 생명과학대학 식품공학부) ;
  • 양성용 (고려대학교 생명과학대학 식품공학부) ;
  • 이성희 (고려대학교 생명과학대학 식품공학부) ;
  • 노수환 (고려대학교 생명과학대학 식품공학부) ;
  • 이광원 (고려대학교 생명과학대학 식품공학부)
  • Published : 2009.10.31

Abstract

Lindera obtusiloba Blume (LO), which is widely distributed in Korea, Japan and China, has traditionally been used as a popular folk medicine for the treatment of fever, abdominal pain, bruise and extravation. The purpose of this study was to examine the toxicities of the single and 14-day repeated doses in Sprague-Dawley rats orally administrated with LO at doses of 0, 500, 1000, 2000 (14-day repeated toxicity test) and 5000 (single toxicity test) mg (dry weight)/kg of body weight/day. The results showed that there was no difference in body weight change, food intake, water consumption, or organ weight among different dose groups. Also we observed no death and abnormal clinical signs during the experimental period. Between the groups orally administered LO and the control group, there was no statistical significance in hematological test or serum biochemical values. There were no evidences of histopathological alteration as well as abnormal gross finding mediated by single and 14 days treatments with LO. These results suggest that no observed adverse effect level (NOAEL) of the oral application of LO was considered to be more than 2000 mg/kg in rats under the conditions employed in this study.

References

  1. Korea Health Industry Development Institute. 2006. The tendency of Research and development of internal and external natural source remedies. The second: Plan for promotion of natural medicine research & development. p 1-20.
  2. Park BG, Lee HS, Jung SH, Koo YC, Hong CO, Lee SJ, Lee KW. 2007. Single and 14-day repeated oral toxicity study and genotoxicological safety estimate of plantamajoside isolate from Plantago asiatica. J Toxicol Pub Health 23: 79-86. https://doi.org/10.5487/TR.2007.23.1.079
  3. Park JC, Yu YB, Lee JH. 1996. Isolation and structure elucidation of flavonoid glycosides from Lindera obtusiloba Blume. J Korean Soc Food Nutr 25: 76-79.
  4. Kwon HC, Choi SU, Lee JO, Bae KH, Zee OP, Lee KR. 1999. Two new lignans from Lindera obtusiloba Blume. Arch Pharm Res 22: 417-422. https://doi.org/10.1007/BF02979069
  5. Kwon HC, Baek NI, Choi SU, Lee KR. 2000. New cytotoxic butanolides from Lindera obtusiloba Blume. Chem Pharm Bull 48: 614-426. https://doi.org/10.1248/cpb.48.614
  6. Ishii R, Yoshikawa K, Minakata H, Komura H, Kada T. 1984. Specificities of bio-antimutagens in plant kingdom. Agric Biol Chem 48: 2587-2591. https://doi.org/10.1271/bbb1961.48.2587
  7. Niwa M, Iguchi M, Yamamura S. 1975. Three new obtusilactones from Lindera obtusiloba Blume. Chem Lett 655-658. https://doi.org/10.1246/cl.1975.655
  8. Komae H, Hayashi H. 1972. Phytosterols of the trunks of Lindera obtusiloba Blume. Phytochemistry 11: 1182. https://doi.org/10.1016/S0031-9422(00)88485-X
  9. Korea Food & Drug Administration. 2005. The standard of toxicity test for medicine and so on. Korea Food & Drug Administration Notice 2005-60.
  10. Korea Food & Drug Administration. 2005. The Standard for Nonclinical Demonstration Management. Korea Food & Drug Administration Notice 2005-79.
  11. OECD/OCDE. 2001. Guideline for the Testing of Chemicals 423.

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