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약용식물 물 추출물이 Goto-Kakizaki 흰쥐의 간 세포액에서 당대사 관련효소 활성에 미치는 영향

Effect of Medicinal Plant Water Extracts on Glucose-regulating Enzyme Activities in Goto-Kakizaki Rat Liver Cytosol

  • 김대중 (강원대학교 식물생명공학) ;
  • 정미자 (강원대학교 BK21사업단(뉴트라슈티컬바이오)) ;
  • 유진균 (강원대학교 식물생명공학) ;
  • 서동주 (강원대학교 식물생명공학) ;
  • 김정미 (보리나라 기업부설 연구소) ;
  • 최면 (강원대학교 식물생명공학)
  • Kim, Dae-Jung (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • You, Jin-Kyoun (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Seo, Dong-Joo (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Jeong-Mi (Borinara Co., Ltd.) ;
  • Choe, Myeon (Dept. of Plant Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
  • 발행 : 2009.10.31

초록

본 연구에서는 유전적으로 제2형 당뇨를 가진 Goto-Kakizaki 흰쥐의 간 세포액에 함유된 당 이용 대사 관련 주요 효소인 glucokinase(GK)와 acetyl CoA carboxylase (ACC)에 약용 식물 물 추출물들이 어떤 영향을 미치는지 알아보았다. $\alpha$-Glucosidase 저해제는 제2형 당뇨 치료와 예방을 위해 사용되어져 오고 있다. 따라서 약용식물 물 추출물들의 $\alpha$-glucosidase 저해 활성을 알아보았다. Goto-Kakizaki 흰쥐의 간 세포액 분획물을 GK과 ACC 활성을 알아보기 위해 사용하였다. 본 실험에 사용된 약용 식물들은 Lycium chinense(JGP), Discorea japonica Thunb.(SY), Pyrus pyrifolia(YSB), Cornus officinalis(SSY), Paeonia suffruticosa ANDR.(MDP), Cordyceps militaris(DCH) 그리고 Acanthopanax senticosus(GSO)였다. 지골피(JGP), 산약(SY), 야생배(YSB) 그리고 산수유(SSY) 물 추출물들은 GK 활성을 증가시켰고, 실험에 사용된 모든 약용식물 물추출물들은 간 ACC 활성을 증가시켰다. 야생배(YSB), 산수유(SSY), 목단피(MDP) 그리고 가시오가피(GSO) 물 추출물들은 대조군보다 현저히 높은 $\alpha$-glucosidase 활성 억제를 보여주었다. $\alpha$-Glucosidase 활성을 가장 억제시킨 것은 GSO 물 추출물이었고, 그 억제율은 $\alpha$-glucosidase 치료제인 acarbose보다 높았다. 따라서 제2형 당뇨에서 지골피 (JGP), 산약(SY), 야생배(YSB) 그리고 산수유(SSY) 물 추출물들은 글루코스 대사과정을 활성화시켜 항당뇨 효과를 나타내는 천연 소재일 것으로 생각되고 야생배(YBS), 목단피(MDP) 그리고 가시오가피(GSO)는 천연 $\alpha$-glucosidase 저해제로써 사용될 수 있을 것이다. 약용식물 물 추출물에 의한 증가된 ACC 활성은 더해진 항당뇨 효과를 나타낼 것이다.

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피인용 문헌

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  2. Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA vol.46, pp.2, 2013, https://doi.org/10.4163/kjn.2013.46.2.119
  3. Antioxidant Activities and α-Glucosidase Inhibitory Effect of Water Extracts from Medicinal Plants vol.21, pp.3, 2013, https://doi.org/10.7783/KJMCS.2013.21.3.197