Antimicrobial Effect of Edible Pullulan Film Containing Natural Antimicrobial Material on Cariogenic Bacteria

천연물 유래 항균물질을 포함한 가식성 Pullulan 필름의 충치유발균에 대한 항균효과

  • Kim, Ki-Myong (Jeonnam Biofood Technology Center) ;
  • Hwang, Kwon-Tack (Dept. of Food & Nutrition, Nambu University) ;
  • You, Sang-Guan (Dept. of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Ung-Soo (Division of Food and Biotechnology, Chungju National University) ;
  • Jung, Kyung-Hwan (Division of Food and Biotechnology, Chungju National University) ;
  • Moon, Sung-Kwon (Division of Food and Biotechnology, Chungju National University) ;
  • Choi, Won-Seok (Division of Food and Biotechnology, Chungju National University)
  • 김기명 (전라남도 식품산업연구센터) ;
  • 황권택 (남부대학교 식품영양학과) ;
  • 유상권 (강릉원주대학교 해양생명공학부) ;
  • 이웅수 (충주대학교 식품생명공학부) ;
  • 정경환 (충주대학교 식품생명공학부) ;
  • 문성권 (충주대학교 식품생명공학부) ;
  • 최원석 (충주대학교 식품생명공학부)
  • Published : 2009.10.31


This study was focused on the development of anticariogenic edible films using pullulan containing grapefruit seed extract (GFSE), polylysine or propolis. According to the result of antimicrobial activity (disc diffusion method) of GFSE, polylysine and propolis against Streptococcus mutans, antimicrobial pullulan film was produced by adding grapefruit seed extract. The optimum combination of pullulan and sorbitol (plasticizer) was 10$\sim$15% (w/v) and 40$\sim$50% of pullulan (w/w), respectively. Minimum concentration of grapefruit seed extract for growth inhibition of Str. mutans was 50 ppm in medium. Formulation of antimicrobial pullulan films containing grape seed extract was established and these results evidently showed potential for commercial application.


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