Separation of Chromophoric Substance from Amur Cork Tree Using GC-MS

GC-MS를 이용한 황벽의 색소 성분 분리 거동

  • 안춘순 (인천대학교 패션산업학과)
  • Published : 2009.06.30


Amur cork tree was extracted in methanol with the purpose of investigating the most effective extraction procedure for detecting the chromophore using the GC-MS analysis. Different procedures of waterbath and hotplate extractions were carried out and five different GC-MS instrument parameters including the operating temperatures in the GC capillary column and the MSD scan range were tested for their efficiencies. Berberine was determined by the detection of dihydroberberine at 15.0 min r.t. Hotplate was a better device for extracting amur cork tree than waterbath shaker either with or without presoaking in the room temperature. Water was not an adequate extraction medium for the berberine detection. The most effective GC-MS parameter was Method 4; the initial temperature at $50^{\circ}C$ followed by the temperature increase of $23^{\circ}C$/min until $210^{\circ}C$, then increase of $30^{\circ}C$/min until the final temperature reach at $305^{\circ}C$, then hold for 14 minutes to maintain the total run time 24.12 minutes. The MSD scan range for Method 4 was $35\sim400$m/z.


  1. Ahn, C., & Obendorf, S. K. (2003). Separation of chromophoric chromophoric substance from madder plant under different extraction and analytical conditions. Journal of the Korean Society of Clothing & Textiles, 27(11), 1350-1357
  2. Ahn, C., & Obendorf, S. K. (2004). Dyes on archaeological textiles: Analyzing alizarin and its degradation products. Textile Research Journal, 74(11), 949−954
  3. Ahn, C., & Obendorf, S. K. (2006). GC-MS analysis of dyes extracted from turmeric. Fibers and Polymers, 7(2), 158−163
  4. Budavari, S., O'Neil, M. J., Smith, A., Heckelman, P. E., & Kinneary, J. F. (1996). The merck index. Whitehouse Station, N.J: Merck & Co, Inc
  5. Candan, F., Unlu, M., Tepe, B., Daferera, D., Polissiou, M., Sokmen, A., & Akpulat, H. A. (2003). Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). Journal of Ethnopharmacology, 87, 215−220
  6. Choi, I. J. (2005). GC/MS를 이용한 현장의 유기미지시료 분석. OSHRI, Korea Occupational Safety and Health Agency. Retrieved May 10, 2009, from
  7. Fabbri, D., Chiavari, G., & Ling H. (2000). Analysis of anthraquinoid and indigoid dyes used in ancient artistic works by thermally assisted hydrolysis and methylation in the presence of tetramethylammonium hydroxide. Journal of Anal. Appl. Pyrolysis, 56, 167−178
  8. Giles, C. H. (1965). The fading of colouring matters. Journal of Applied Chemistry, 15, 541−550
  9. Nam, S. W. (1999). Natural dyeing class. Laboratory of Natural dyeing. Suwan: Sungkeunkwan University
  10. Ong, E. S., & Len, S. M. (2003). Pressurized hot water extraction of berberine, baicalein and glycyrrhizin in medicinal plants. Analytica Chimica Acta, 482, 81−89
  11. Ong, E. S., Woo, S. O., & Yong, Y. L. (2000). Pressurized liquid extraction of beberine and aristolochic acids in medicinal plants. Journal of Chromatography A, 313, 57−64
  12. Orska-Gawry, J., Surowiec, I., Kehl, J., Rejniak, H., Urbaniak- Walczak, K., & Trojanowicz, M. (2003). Identification of natural dyes in archaeological Coptic textiles by liquid chromatography with diode array detection. Journal of Chromatography A, 989, 239−248
  13. Schweppe, H. (1989). Identification of red madder and insect dyes by thin-layer chromatography. In S. H. Zeronian & H. L. Needles (Eds.), Historic textile and paper materials II: Conservation and characterization (pp. 188-219). Washington, DC: American Chemical Society
  14. So, H. O. (1996). The study of phellodendron amurense rupr. dyeing. The Korean Society of Costume, 28, 121−132
  15. Song, J. F., He, Y. Y., & Guo, W. (2002). Polarographic deteremination of berberine in the presence of $H_2O_2$ in medicinal plants. Journal of Pharmaceutical and Biomedical Analysis, 28, 355−363
  16. Tokusoglu, O., Unal, M. K., & Yildirim, Z. (2003). HPLCUV and GC-MS characterization of the flavonol aglycons quercetin, kaempferol, and myricetin in tomato pastes and other tomato-based products. Acta Chromatographica, 13, 196−207
  17. Turner, N., Li, J. Y., Gosby, A., To, S. W. C., Cheng, Z., Miyoshi, H., Taketo, M. M., Cooney, G. J., Kraegen, E. W., James, D. E., Hu, L. H., Li, J., & Ye, J. M. (2008a). Berberine and its more biologically available derivative, dihydroberberine, inhibit mitochondrial respiratory complex I. Diabetes, 57(5), 1414−1418
  18. Turner, N., Li, J. Y., Gosby, A., To, S. W. C., Cheng, Z., Miyoshi, H., Taketo, M. M., Cooney, G. J., Kraegen, E. W., James, D. E., Hu, L. H., Li, J., & Ye, J. M. (2008b). Online Appendix. Supplementary methods. Diabetes. Retrieved May 20, 2009, from
  19. Walton, P., & Taylor, G. (1991). The characterisation of dyes in textiles from archaeological excavations. Chromatography and Analysis, June, 5−7

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