Inhibitory Effect of Muscat Bailey A Seed Extract on Melanin Production in $\alpha$-Melanin Stimulating Hormone-stimulated B16 Cell

머루포도 씨 추출물의 $\alpha$-Melanin Stimulating Hormone으로 자극한 B16세포에서 melanin 생성억제 효과

  • Lee, Pyeong-Jae (Department of Natural Medicine Resources, Semyung University)
  • 이평재 (세명대학교 자연약재과학과)
  • Published : 2009.10.31

Abstract

Inhibitory effect of skin and seed of three species grape cultivated in Korea on melanogenesis was investigated. Melanin generation was examined in $\alpha$-Melanin Stimulating Hormone-stimulated B16 cell, mouse melanoma, in the presence of samples. All skin sample did not show the inhibitory effect. Seed extract of Campbell early and Neo Muscat had negative effect on cell viability. When $50{\mu}g/ml$ seed extract of Muscat Bailey A was treated, amount of generated melanin and cell viability were $51.6{\pm}20.5%$ and $90.4{\pm}11.3%$ compared to control, respectively. Seed extract of Muscat Bailey A reduced the tyrosinase protein induced by $\alpha$-Melanin Stimulating Hormone, which suggests that inhibitory effect of seed extract of Muscat Bailey A on melanin is partly due to suppression of tyrosinase that is responsible for melanin production.

References

  1. Abdel-Malek, Z., V.B. Swope, I. Suzuki, C. Akcali, M.D. Harriger, S.T. Boyce, K. Urabe and V.J. Hearing. 1995. Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc. Natl. Acad. Sci. USA. 92:1789-1793 https://doi.org/10.1073/pnas.92.5.1789
  2. Hosoi, J., E. Abe, T. Suda and T. Kuroki. 1985. Regulation of melanin synthesis of B16 mouse melanoma cells by 1 alpha, 25-dihydroxyvitamin D3 and retinoic acid. Cancer Res. 45:1474-1478
  3. Jang, J.Y., J.H. Lee, B.W. Kang, K.T. Chung, Y.H. Choi and B.T. Choi. 2008. Dichloromethane fraction of Cimicifuga heracleifolia decreases the level of melanin synthesis by activating the ERK or AKT signaling pathway in B16F10 cells. Exp. Dermatol. 18:232-237 https://doi.org/10.1111/j.1600-0625.2008.00794.x
  4. Kar, P., D. Laight, K.M. Shaw and M.H. Cumming. 2006. Flavonoid-rich grapeseed extracts: a new approach in high cardiovascular risk patients?. Int. J. Clin. Pract. 60:1484-1492 https://doi.org/10.1111/j.1742-1241.2006.01038.x
  5. Kim, D.S., Y.M, Jeong, I.K. Park, H.G. Hahn, H.K. Lee, S.B. Kwon, J.H. Jeong, S.J. Yang, U.D. Sohn and K.C. Park. 2007. A New 2-Imino-1,3-thiazoline Derivative, KHG22394, Inhibits Melanin Synthesis in Mouse B16 Melanoma Cells. Biol. Pharm. Bull. 30:180-183 https://doi.org/10.1248/bpb.30.180
  6. Lee, J., E. Jung, J. Lee, S. Huh, Y.C. Boo, C.G. Hyun, Y.S. Kim and D. Park. 2007. Mechanism of melanogenesis inhibition by 2,5-dimethyl-4-hydroxy-3(2H)-furanone. Br. J. Dermatol. 157:242-248 https://doi.org/10.1111/j.1365-2133.2007.07934.x
  7. Maeda, K., T. Naitou, K. Umishio, T. Fukukara and A. Motoyama. 2007. A Novel Melanin Inhibitor: Hydroperoxy Traxastane-Type Triterpene from Flowers of Arnica montana. Biol. Pharm. Bull. 30:873-879 https://doi.org/10.1248/bpb.30.873
  8. Miyagi Y., K. Miwa and H. Inoue. 1997. Inhibition of human low-density lipoprotein oxidation by flavonoids in red wine and grape juice. Am. J. Cardiol. 80:1627-31 https://doi.org/10.1016/S0002-9149(97)00755-8
  9. Miyamura, Y., S.G. Coelho, R. Wolber, S.A. Miller, K. Wakamatsu, B.Z. Zmudzka, S. Ito, C. Smuda, T. Passeron, W. choi, J. Batzer, Y. Yamaguchi, J.Z. Beer and V.J. Hearing. 2006. Regulation of human skin pigmentation and responses to ultraviolet radiation. Pigment. Cell Res. 20:2-13 https://doi.org/10.1111/j.1600-0749.2006.00358.x
  10. Oh Y.S., J.H. Lee, S.H. Yoon, C.H. Oh, D.S. Choi, E. Choe and M.Y. Jung. 2008. Characterization and Quantification of Anthocyanins in Grape Juices Obtained from the Grapes Cultivated in Korea by HPLC/DAD, HPLC/MS, and HPLC/MS/MS. J. Food Sci. 73:378-389
  11. Ohguchi, K., Y. Akao and Y. Nozawa. 2005. Involvement of calpain in melanogenesis of mouse B16 melanoma cells. Mol. Cell. Biochem. 275:103-107 https://doi.org/10.1007/s11010-005-1081-0
  12. Schwahn, D.J., W. Xu, A.B. Herrin, E.S. Bales and E.E. Medrano. 2001. Tyrosine levels regulate the melanogenic response to alpha-melanocyte-stimulating hormone in human melanocyte: Implications for pigmentation and proliferation. Pigment. Cell Res. 14:32-39 https://doi.org/10.1034/j.1600-0749.2001.140106.x
  13. Solano, F., S. Briganti, M. Picardo and G. Ghanem. 2006. Hypopigmenting agents: an updated review on biological, chemical and clinical aspects. Pigment Cell Res. 19:550-571 https://doi.org/10.1111/j.1600-0749.2006.00334.x
  14. Suzuki, I., R.D. Cone, S. Im, J. Nordlund and Z.A. Abdelmalkek. 1996. Binding of Melanotropic Hormones to the Melanocortin Receptor MC1R on Human Melanocytes Stimulates Proliferation and Melanogenesis. Endocrinology. 137:1627-1633 https://doi.org/10.1210/en.137.5.1627
  15. Vitseva, O., S. Varghese, S. Chakrabarti, J.D. Folts and J.E. Freedman. 2005. Grapeseed and skin extracts inhibits platelet function and release of reactive oxygen intermediates. J. Cardiovasc. Pharmacol. 46:445-451 https://doi.org/10.1097/01.fjc.0000176727.67066.1c
  16. Yamaguchi, Y., M. Brenner and V.J. Hearing. 2007. The Regulation of Skin Pigmentation. J. Biol. Chem. 282:27557-27561 https://doi.org/10.1074/jbc.R700026200
  17. Yamakoshi, L., F. Otsuka, A. Sano, S. Tokutake and M. Saito. 2003. Lightening Effect on Ultraviolet-Induced Pigmentation of Guinea Pig Skin by Oral Administration of a Proanthocyanidin-Rich Extract from Grape Seeds. Pigment. Cell Res. 16:629-638 https://doi.org/10.1046/j.1600-0749.2003.00093.x