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Study on Microbiochip for Buccal Cell Lysis and DNA Purification

상피세포 시료 전처리용 마이크로바이오칩에 관한 연구

  • Ha, Seung-Mo (Dept. of Mechanical Engineering, Hanyang Univ.) ;
  • Cho, Woong (Dept. of Mechanical Engineering, Hanyang Univ.) ;
  • Ahn, Yoo-Min (Dept. of Mechanical Engineering, Hanyang Univ.) ;
  • Hwang, Seung-Yong (Division of Molecular and Life Science, Hanyang Univ.)
  • 하승모 (한양대학교 기계공학과) ;
  • 조웅 (한양대학교 기계공학과) ;
  • 안유민 (한양대학교 기계공학과) ;
  • 황승용 (한양대학교 분자생명과학부)
  • Received : 2009.07.13
  • Accepted : 2010.10.10
  • Published : 2010.12.01

Abstract

This paper describes a separable microfluidic device fabricated with PDMS (polydimethylsiloxane) and glass. The device is used for sample preparation involving cell lysis and the DNA purification process. The cell lysis was performed for 2 min at $80^{\circ}C$ in a serpentine-type microreactor ($20 {\mu}l$) using a Au microheater that was integrated with a thermal microsensor on a glass substrate. The DNA that was mixed with other residual products during the cell lysis process was then filtered through a new filtration system composed of microbeads (diameter: $50 {\mu}m$) and PDMS pillars. Since the entire process (sample loading, cell lysis reaction, DNA purification, and sample extraction) was performed within 5 min in a microchip, we could reduce the sample preparation time in comparison with that for the conventional methods used in biochemistry laboratories. Finally, we verified the performance of the sample preparation chip by conducting PCR (polymerase chain reaction) analysis of the chip product.

Keywords

Microbiochip;Sample Preparation;Cell Lysis;DNA Purification

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