Antimicrobial Activities of Methanol Extracts Obtained from Several Ferns

양치식물류의 메탄올 추출물에 항균활성 분석

  • Shin, So-Lim (Department of Horticulture, Chungbuk National University) ;
  • Lee, Cheol-Hee (Department of Horticulture, Chungbuk National University)
  • Received : 2010.06.28
  • Accepted : 2010.10.02
  • Published : 2010.10.30

Abstract

Methanol extracts of the aerial and rhizome parts obtained from ten species of ferns has been screened for antimicrobial activities against Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Propionibacterium acnes, Streptococcus mutans and Streptococcus sobrinus. Antimicrobial activities were carried out using broth microdilution method and paper disc diffusion assay and the extracts which showed clear zones more than 15mm in concentration of 2 mg/disc were tested for its antimicrobial activities at the $0.125{\sim}2\;mg{\cdot}mL^{-1}$ concentration of each extract for three days. The non-sterilized crude methanol extracts of Osmunda cinnamomea var. fokiensis rhizome showed the highest antimicrobial activities on B. subtilis(39%), E. coli (33%) and L. monocytogenes(58%) at the concentration of $2\;mg{\cdot}mL^{-1}$ after 72 hours. In P. acnes, frond extract of O. cinnamomea var. fokiensis showed most vigorous antimicrobial activities in the all extracts but it showed weak activity(clear zone diameters below 15 mm). All extracts has the antimicrobial activities on Streptococcus, but they exhibited weak activity. At the concentration of $2\;mg{\cdot}mL^{-1}$, only Osmunda japonica rhizome extracts showed 28 and 39% of antimicrobial activities on S. mutans and S. sobrinus after 72 hours and the other extracts showed below 10% of antimicrobial activities on S. mutans and S. sobrinus.

Acknowledgement

Supported by : 충북대학교

References

  1. Agrios, G. N. 1988. Plant Pathology. Academic Press. Inc., New York. p. 325-450.
  2. Ahn, D.J., Y.S. Kwak, M.J. Kim, J.C. Lee, C.S. Shin, and K.T. Jeong. 2000. Sceering of herbal plant extracts showing antimicrobial activity against some food spoilage and pathogenic microorganisms. J. Medic. Crop Sci. 8:109-116(in Korean).
  3. Bae, J.H. 2005. Antimicrobial effect of Hedyotis diffusa extracts on food-born pathogens. J. Kor. Soc. Food Sci. Nutr. 34:107-112(in Korean). https://doi.org/10.3746/jkfn.2005.34.1.107
  4. Boussaada, O., S. Ammar, D. Saidana, J. Chriaa, I. Chraif, M. Daami, A.N. Helal, and Z. Mighri. 2008. Chemical composition and antimicrobial activity of volatile components from capitula and aerial parts of Rhaponticum acaule DC growing wild in Tunisia. Microbiol. Res.163:87-95. https://doi.org/10.1016/j.micres.2007.02.010
  5. Choi, Y.H., H.A. Bae, E.K. Eo, and G.Y. Jung. 2007. Death due to sepsis in emphysematous cholecystitis. J. Kor. Surg. Soc. 72:250-253(in Korean).
  6. Evanoff, G.V., C.S. Thompson, R. Foley, and E.J. Weinman. 1987. Spectrum of gas within the kidney: emphysematous pyelonephritis and emphysematous pyelitis. Am. J. Med. 83:149-54. https://doi.org/10.1016/0002-9343(87)90511-0
  7. Hemady, R., M. Zaltas, B. Paton, C.S. Foster, and A.S. Baker. 1990. Bacillus-induced endophthalmitis: new series of 10 cases and review of the literature. Br. J. Ophthalmol. 74:26-29. https://doi.org/10.1136/bjo.74.1.26
  8. Huang, J.J. and C.C. Tseng. 2000. Emphysematous pyelonephritis: clinicoradiological classification, management, prognosis, and pathogenesis. Arch. Intern. Med. 160:797-805. https://doi.org/10.1001/archinte.160.6.797
  9. Jeon, Y.O., K.H. Kim, S.I. Kim, and Y.S. Han. 1998. Screening if antimicrobial activity of the plantain(Plantago asiatica L.) extract. Kor. J. Soc. 14: 498-502(in Korean).
  10. Kim, H.J., H.W. Lim, S.W. Choi, and C.S. Yoo. 2006. Antimicrobial effect of ethanol extract of Dryopteris crassirhizoma Nakai on Propionibacterium acnes. J. Soc. Cosmet. Scient. Kor. 32:201-208(in Korean).
  11. Kim, Y.S., H.J. Lee, J.R. Lee, B.K. Kang, W.I. Lee, Y.C. Kil, J.T. Suh, and H.J. Suh. 2000. Comparative evaluation of fluconazole susceptibility methods for Candida species -Broth microdilution test, E test and disk diffusion test-. Kor. J. Clin. Pathol. 20:36-40(in Korean).
  12. Kitzberger, C.S.G., A. Smania Jr., R.C. Pedrosa, and S.R.S. Ferreira. 2007. Antioxidant and antimicrobial activities of shiitake(Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J. Food Engin. 80:631–638.
  13. Layton, A.M. 2006. A review on the treatment of acne vulgaris. Int. J. Clin. Pract. 60:64-72.
  14. Lee, M.H. 2005. Antibiotics. Shin-Il Books, Seoul(in Korean).
  15. Lee, S.G., J.B. Cho, Y.B. Shon, S.W. Park, S.J. Kim, D.K. Jin, and K.H. Paik. 2008. Peritoneal dialysis associated peritonitis and empirical antibiotics therapy in Korean children with chronic renal failure. J. Kor. Soc. Pediatr. Nephrol. 12:213-220(in Korean). https://doi.org/10.3339/jkspn.2008.12.2.213
  16. Lee, S.J., S.J. Kim, Y.H. Choi, Y.N. Woo, B.W. Kim, S.G. Chang, M.E. Kim, C.S. Kim, J.G. Lee, B.S. Sim, H.J. Kim, B.H. Chung, I.R. Cho, and S.D. Lee. 2007. Safety of Uro-Vaxom treatment for patients with recurrent cystitis: an open multicenter study. Kor. J. Urol. 48:428- 432(in Korean). https://doi.org/10.4111/kju.2007.48.4.428
  17. Leeming, J.P., J.E. Sanaim, and J.L. Burtion. 1997. Susceptibility of Malassezia furfur subgroups to terbinafine. Br. J. Dermatol. 137:764-767. https://doi.org/10.1111/j.1365-2133.1997.tb01115.x
  18. Lim, W.H., J.H. Shin, S.P. Suh, and D.W. Ryang. 1998. Diagnosis of central venous catheter-related sepsis using differential quantitative blood cultures. Kor. J. Clin. Pathol. 18:208-214(in Korean).
  19. Mandell, G.L., J.E. Bennett, and R. Dolin. 2000. Principles and practice of infectious disease. Curchill Livingstone, Philadelphia.
  20. Michielin, E.M.S., A.A. Salvador, C.A.S. Riehl, A.Smânia Jr., E.F.A. Smânia, and S.R.S. Ferreira. 2009. Chemical composition and antibacterial activity of Cordia verbenacea extrats obtained by different methods. Biores. Thechnol. 100:6615-6623. https://doi.org/10.1016/j.biortech.2009.07.061
  21. Moon, S.K., D.H. Shin, J.S. Choi, K.H. Kim, and K.J. Kim. 2008a. Antifungal susceptibility testing of derm,atophytes. Kor. J. Med. Mycol. 13:61-74(in Korean).
  22. Moon, Y.G., C.Y. Song, I.K. Yeo, G.Y. Kim, and M.S. Heo. 2008b. Antibacterial activities of Suaeda maritima extract. J. Life Sci. 18:776-781(in Korean). https://doi.org/10.5352/JLS.2008.18.6.776
  23. Park, B.G., I.S. Chun, Y.P. Rhee, S.Y. Choi, K.R. Kim, S.H. Jang, J.Y. Hwang, D.J. Choi, B.G. Seo, S.H. Kim, and S.J. Kim. 1997. A case of Listeria monocytogenes endocarditis in apparently healthy adult. Kor. Circul. J. 27:671-676(in Korean). https://doi.org/10.4070/kcj.1997.27.6.671
  24. Ryan, K.J. and C.G. Ray. 2004. Sherris medical microbiology. McGraw Hill, New York.
  25. Shin, J.H., S.K. Sung, J.D. Shim, H.R. Kim, S.W. Park, and J.N. Lee. 2005. A case of Paenibacillus alvei cellulitis in immunocompetent patient. Kor. J. Lab. Med. 25:53-55(in Korean).
  26. Shinozaki, J., M. Shibuya, K. Masuda, and Y. Ebizuka. 2008. Squalene cyclase and oxidosqualene cyclase from a fern. FEBS Lett. 582:310-318. https://doi.org/10.1016/j.febslet.2007.12.023
  27. Smith-Palmer, A., J. Stewart, and L. Fyfe. 1998. Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett. Appl. Microbiol. 26:118-122. https://doi.org/10.1046/j.1472-765X.1998.00303.x
  28. Vazquez-Boland, J.A., M. Kuhn, P. Berche, T. Charaborty, G. Dominguez-Bernal, W. Goebel, B. Gonzalez-Zorn, J. Wehland, and J. Kreft. 2001. Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 14:584-640. https://doi.org/10.1128/CMR.14.3.584-640.2001
  29. Whiley, R.A. and D. Beighton. 1998. Current classification of the oral streptococci. Oral Microbiol. Immunol. 13, 195-216. https://doi.org/10.1111/j.1399-302X.1998.tb00698.x
  30. Zeng-fu, L.I., H. Huil, Z. Hang-yi, and Z. Jun-chen. 2008. Review on the extraction of flavonoids from fern. J. Sanm. Univ. 25:22(in Chinese).
  31. Zhao, Z., J. Jin, J. Ruan, C. Zhu, C. Lim, W. Fang, and Y. Cai. 2007. Antioxidant flavonoid glycosides from aerial parts of the fern Abacopteris penangiana. J. Nat. Prod. 70:1683-1686. https://doi.org/10.1021/np0703850