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Detection of Pathogenic Salmonella with a Composite Quantum Dot

나노 양자점 결합을 이용한 살모넬라 식중독균 검출

  • Received : 2010.12.03
  • Accepted : 2010.12.14
  • Published : 2010.12.25

Abstract

It is required to develop rapid methods to identify pathogenic Salmonella in food products for protecting and maintaining safety of the public health from Salmonellosis. The objective of the present study was to explore feasibility of the nanotechnology to detect pathogenic Salmonella rapidly in various samples. Sensitivity of the a composite quantum dot to detect Salmonella typhimurium in samples were evaluated. For selective detection of Salmonella, anti-Salmonella polycolonal antibody was utilized to capture and stain Salmonella. Quantum dots were attached onto Salmonella in the samples and produced fluorescent light. Fluorescence response of the composite quantum dot was measured with a commercial fluorescence meter. The fluorescence signal starts to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6\;CFU/mL$ Salmonella spiked in PBS.

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