- Volume 39 Issue 12
DOI QR Code
Protective Effects of Sasa Borealis Leaves Extract on High Glucose-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells
혈관내피세포에서 고농도 포도당으로 유도된 산화스트레스에 대한 조릿대잎 추출물의 보호효과
- Hwang, Ji-Young (Dept. of Food Science and Nutrition, Pusan National University) ;
- Han, Ji-Sook (Dept. of Food Science and Nutrition, Pusan National University)
- Received : 2010.09.06
- Accepted : 2010.11.29
- Published : 2010.12.31
This study was designed to investigate the protective effects of Sasa borealis leaves on high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Freeze-dried Sasa borealis leaves were extracted with 70% methanol and followed by a sequential fractionation with dicholoromethan, ethyl acetate, butanol and water. The ethyl acetate fraction from Sasa borealis leaves extract (ESLE) was used in this study because it possessed the strongest antioxidant activity among the various solvent fractions. Exposure of HUVECs to 30 mM high glucose for 48 hr resulted in a significant (p<0.05) decrease in cell viability, glutathion (GSH) concentration, activities of antioxidant enzymes including superoxide dimutase (SOD), glutathion peroxidase (GSH-px) and catalase, and a significant (p<0.05) increase in intracellular ROS and lipid peroxidation formation in comparison to the cells treated with 5.5 mM glucose. ESLE treatment decreased intracellular ROS and lipid peroxidation formation and increased cell viability, GSH concentration and expressions of SOD and catalase in HUVECs. These results suggest that ESLE may be able to protect HUVECs from high glucose-induced oxidative stress, partially through the antioxidative defense systems.
Supported by : 부산대학교
- Ross R. 1995. Cell biology of atherosclerosis. Annu Rev Physiol 57: 791-804. https://doi.org/10.1146/annurev.ph.57.030195.004043
- Luscher TF, Baron M. 1997. Biology of endothelium. Clin Cardiol 20: 3-10.
- Hsueh WA, Quinones MJ, Creager MA. 1997. Endothelium in insulin resistance and diabetes. Diabetes Rev 5: 343-352.
- Carlos TM, Harlan JM. 1994. Leukocyte-endothelial adhesion molecules. Blood 84: 2068-2101.
- Calver A, Collier J, Vallance P. 1992. Inhibition and stimulation of nitric oxide synthesis in the human forearm arterial bed of patients with insulin-dependent diabetes. J Clin Invest 90: 2548-2554. https://doi.org/10.1172/JCI116149
Ha SW, Yeo HJ, Bae JS, Chung SC, Kim JG, Kim IS, Lee IK, Kim BW. 2003. Effect and mechanism of high glucose level on the expression of an adhesion protein,
$\betaig-h3$, and cellular function in endothelial cells. J Korean Diabetes Assoc 27: 323-331.
- Peng J, Jones GL, Watson K. 2002. Stress proteins as biomarkers of oxidative stress: effects of antioxidant supplements. Free Radic Biol Med 28: 1598-1606.
- Nuttall SL, Kendall MJ, Martin U. 1999. Antioxidant therapy for prevention of cardiovascular disease. Q J Med 92:239-244. https://doi.org/10.1093/qjmed/92.5.239
- Son SM, Kim IJ, Kim YK. 2000. Study on role of neutrophil in endothelial cell injury under high glucose condition. J Korean Diabetes Assoc 24: 652-665.
- Nandita S, Rajini PS. 2004. Free radical scavenging activity of an aqueous extract of potato peel. Food Chem 85: 611-616. https://doi.org/10.1016/j.foodchem.2003.07.003
- Jeong EY. 2006. Effect of the Sasa borealis leaves extract on metabolic syndrome in C57BL/6J mice fed a high fat diet. MS Thesis. Chonnam National University, Gwangju, Korea. p 1-3.
- Hwang JY, Han JS. 2007. Inhibitory effects of Sasa borealis leaves extracts on carbohydrate digestive enzymes and postprandial hyperglycemia. J Korean Soc Food Sci Nutr 36: 1391-1398. https://doi.org/10.3746/jkfn.2007.36.8.989
- Ko BS, Jun DW, Jang JS, Kim JH, Park SM. 2006. Effect of Sasa borealis and white lotus roots and leaves on insulin action and secretion in vitro. Korean J Food Sci Technol 38: 114-120.
- Im MS. 1984. Studies on pharmacological actions on Sasa borealis Makino. MS Thesis. Sookmyung Woman's University, Seoul, Korea. p 1-2.
- Yoon KD, Kim CY, Huh H. 2000. The flavone glycosides of Sasa borealis. Kor J Pharmacogn 31: 224-227.
- Fautz R, Husen B, Hechenberger C. 1991. Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS). Mutat Res 253: 173-179. https://doi.org/10.1016/0165-1161(91)90130-Z
- Wang H, Joseph JA. 1999. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 27: 612-616. https://doi.org/10.1016/S0891-5849(99)00107-0
- Fraga CG, Leibovita RM, Roeder RG. 1988. Lipid peroxidation measured as thiobarbituric-reactive substances in tissue slices: characterization and comparision with homogenates and microsomes. Free Radic Biol Med 4: 155-161.
- Tietze F. 1969. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissue.Anal Biochem 27: 502-522. https://doi.org/10.1016/0003-2697(69)90064-5
- Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Ann Biochem 72: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
- Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in antioxidant of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
- Aebi H. 1984. Catalase in vitro. Methods Enzymol 105:121-126. https://doi.org/10.1016/S0076-6879(84)05016-3
- Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958. https://doi.org/10.1016/0006-291X(76)90747-6
- Kwon MJ, Jung HS, Kim MK, Kang SH, Seo GW, Song JK, Yoon TY, Jeon MK , Ha TH, Yoon CS, Lee WJ, Noh JH, Kwon SK, Kim DJ, Koh KS, Rhee BD, Lim KH, Lee SH, Park JH. 2007. Cytoprotective effect by antioxidant activity of quercertin in INS-1 cell line. J Korean Diabetes Assoc 31: 383-390. https://doi.org/10.4093/jkda.2007.31.5.383
- Kim MK, Jung HS, Yoon CS, Kwon MJ, Koh KS, Rhee BD, Park JH. 2008. The protective effect of EGCG on INS-1 in the oxidative stress and mechanism. J Korean Diabetes Assoc 32: 121-131. https://doi.org/10.4093/kdj.2008.32.2.121
- Ge QM, Dong Y, Su Q. 2010. Effects of glucose and advanced glycation end products on oxidative stress in MIN6 cells. Cell Mol Biol 9: 1231-1238.
- Rosen P, Nawroth PP, King G, Moller W, Tritschler HJ, Packer L. 2001. The role of oxidative stress in the onset and progression of diabetes and its complications. Diabetes Metab Res Rev 17: 189-212. https://doi.org/10.1002/dmrr.196
- Yi NR, Park MS, Han JS. 2010. Protective effects of fermented soymilk extract on high glucose-induced oxidative stress in human umbilical vein endothelial cells. J Food Sci Nutr 15: 7-13. https://doi.org/10.3746/jfn.2010.15.1.007
- Wang GF, Wu SY, Xu W, Jin H, Zhu ZG, Li ZH, Tian YX, Zhang JJ, Rao JJ, Wu SG. 2010. Geniposide inhibits high glucose-induced cell adhesion through the NF-kappaB signaling pathway in human umbilical vein endothelial cells. Acta Pharmacol Sin 31: 953-962. https://doi.org/10.1038/aps.2010.83
- Sevanian A, Hochstein P. 1985. Mechanism and consequence of lipid peroxidation in biological systems. Annu Rev Nutr 5: 365-390. https://doi.org/10.1146/annurev.nu.05.070185.002053
- Jee JH. 2007. Antidiabetic and oxidative stress relieving effects of fermented red ginseng. MS Thesis. Pusan National University, Busan, Korea. p 39-40.
- Park HY. 2007. Studies on increased antioxidative functionality of seolitae chungkukjang added with green tea powder. MS Thesis. Pusan National University, Busan, Korea. p 3-5.
- Andersen HR, Nielsen JB, Nielsen F, Grandjean P. 1997. Antioxidative enzyme activities in human erythrocytes. Clin Chem 43: 562-568.
- Suttorp N, Toepfer W, Roka L. 1986. Antioxidant defense mechanisms of endothelial cells: glutathione redox cycle versus catalase. Am J Physiol 251: 671-680. https://doi.org/10.1152/ajpcell.1986.251.5.C671
- Merrily A. 2003. Oxygen free radicals antioxidant. Am J Nutr 103: 58-62.