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Effect of Ecklonia cava Water Extracts on Inhibition of IgE in Food Allergy Mouse Model

식품 알레르기 마우스 모델에서 감태 물 추출물의 IgE 분비 억제 효과

  • Song, Eu-Jin (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Lee, Chung-Jo (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Jung, Ji-Yeon (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Kwak, Ji-Hee (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Choi, Moon-Kyoung (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ji (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University) ;
  • Ahn, Dong-Hyun (Dept. of Food Science and Technology/ Institute of Food Science, Pukyong National University)
  • 송유진 (부경대학교 식품공학과/식품연구소) ;
  • 이청조 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 정지연 (부경대학교 식품공학과/식품연구소) ;
  • 곽지희 (부경대학교 식품공학과/식품연구소) ;
  • 최문경 (부경대학교 식품공학과/식품연구소) ;
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2010.09.13
  • Accepted : 2010.11.25
  • Published : 2010.12.31

Abstract

This research was done to verify the effect of Ecklonia cava water extracts (ECWE) on inhibition of allergic reactions using ovalbumin (OVA)-immunized food allergy mouse model. For in vitro test, $10\sim100{\mu}g$/mL of ECWE and OVA were added to splenocytes obtained from OVA-immunized mice. The significant reduction of IgE antibody level in culture supernatants of splenocytes was shown in ECWE adding group at all tested concentrations. In addition, ECWE decreased IL-4 and IFN-$\gamma$ levels in supernatants of splenocytes. To confirm the effect of ECWE in in vivo test, ECWE was injected to peritoneal cavity of OVA-immunized mice. Subsequently, IgE level was measured in serum and cultured supernatants of splenocytes. As a result, the injection of ECWE (5 and 10 mg/kg BW) significantly attenuated the secretion of IgE antibody in both serum and splenocytes. In conclusion, the present study indicates that ECWE could suppress in a food allergy mouse model through the inhibition of IgE secretion.

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