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Catabolic Enzyme Activities and Physiological Functionalities of Lactic Acid Bacteria Isolated from Korean Traditional Meju

재래식 메주에서 분리한 유산균들의 각종 효소활성 및 기능성

  • Jeong, Ji-Kang (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Zheng, Yanfei (Dept. of Food Science and Nutrition, Pusan National University) ;
  • Choi, Hye-Sun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Gwi-Jung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Kun-Young (Dept. of Food Science and Nutrition, Pusan National University)
  • 정지강 (부산대학교 식품영양학과) ;
  • 정연비 (부산대학교 식품영양학과) ;
  • 최혜선 (농촌진흥청 국립농업과학원) ;
  • 한귀정 (농촌진흥청 국립농업과학원) ;
  • 박건영 (부산대학교 식품영양학과)
  • Received : 2010.11.18
  • Accepted : 2010.11.30
  • Published : 2010.12.31

Abstract

Three kinds of Korean traditional Meju were selected and the counts of microorganisms in these Meju were determined. The counts of total aerobic bacteria, lactic acid bacteria and yeasts and molds were 107~108, 106~108 and 107~108 cfu/g, respectively in three Meju and lactic acid bacteria were important microorganisms in the fermentation of Meju. Therefore, we isolated three kinds of dominant lactic acid bacteria from these Meju. They were identified as Leuconostoc mesenteroides (98%, Lm-SMm), Lactobacillus plantarum (99%, Lp-SMm) and Lactococcus lactis (98%, Ll-GAm). Then, enzyme activities and physiological functionalities of three lactic acid bacteria were investigated. Protease, lipase and $\alpha$-amylase activities were detected in three lactic acid bacteria, Ll-GAm showed relatively higher activities than other two lactic acid bacteria. Lm-SMm, Lp-SMm and Ll-GAm showed 45, 48 and 60% of antioxidative activity to 1,1-diphenyl-2-picryhydrazyl (DPPH), and exhibited 45, 67 and 70% of inhibitory effects in HT-29 human colon cancer cells, respectively. These results indicate that three lactic acid bacteria isolated from traditional Meju, especially Ll-GAm are applicable to Meju preparation for soybean paste industry.

Acknowledgement

Supported by : 농촌진흥청

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