Effect of Intracellular Calcium Chelator on Phosphorylation of Spinal N-Methyl-D-Aspartate Receptor following Electroacupuncture Stimulation in Rats

칼슘 저해제가 전침자극에 의한 척수 N-Methyl-D-Aspartate 수용체 인산화에 미치는 영향

  • Jung, Taek-Guen (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Cho, Sung-Woo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kang, Yeon-Kyeong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Chang, Dong-Ho (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, In-Seon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University)
  • 정택근 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 조성우 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 강연경 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 장동호 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 이인선 (동의대학교 한의과대학 한방재활의학과교실)
  • Received : 2009.12.22
  • Accepted : 2010.01.12
  • Published : 2010.01.31

Abstract

Objectives : We investigated the role of intracellular calcium chelator, bis-(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid(BAPTA), on the modulation of phosphorylation of the spinal N-methyl-D-aspartate receptor(NMDAR) NR1 and NR2B subunits following electroacupuncture(EA). Methods : Bilateral 2 Hz EA stimulation with 1.0 mA was delivered at those acupoints corresponding to Zusanli(ST36) and Sanyinjiao(SP6) in man via needles for 30min. Results : EA analgesia was reduced by intra-peritoneal injection at a higher dose of BAPTA from termination of EA stimulation. At 60 min after EA treatment, the total number of c-fos-immunostained neurons in each regions of the dorsal horn in the $L_{4-5}$ segments was decreased by BAPTA injection, especially in nucleus proprius. The mean integrated optical density (IOD) of NR1 and NR2B subunits were increased only in superficial laminae of EA-treated rats when compared with normal rats. However, the mean IOD of pNR1 was significantly decreased by BAPTA injection in both the superficial laminae and neck region and pNR2B in the superficial laminae. Western blot analyses confirmed the decreased expression of pNR1 and pNR2B. Conclusions : We concluded that intracellular calcium may well play an important role in EA analgesia by modulating the phosphorylation state of spinal NMDAR subunits.

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