칼슘 저해제가 전침자극에 의한 척수 N-Methyl-D-Aspartate 수용체 인산화에 미치는 영향

Effect of Intracellular Calcium Chelator on Phosphorylation of Spinal N-Methyl-D-Aspartate Receptor following Electroacupuncture Stimulation in Rats

  • 정택근 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 조성우 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 강연경 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 장동호 (동의대학교 한의과대학 한방재활의학과교실) ;
  • 이인선 (동의대학교 한의과대학 한방재활의학과교실)
  • Jung, Taek-Guen (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Cho, Sung-Woo (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kang, Yeon-Kyeong (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Chang, Dong-Ho (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Lee, In-Seon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Dong-Eui University)
  • 투고 : 2009.12.22
  • 심사 : 2010.01.12
  • 발행 : 2010.01.31

초록

목 적 : 본 연구는 전침 자극에 의한 척수내 N-methyl-D-aspartate(NMDA) 수용체의 NR1 및 NR2B subunit 인산화에 미치는 세포내 칼슘 저해제 bis-(2-aminophenoxy)-ethane-N,N,N',N'-tetraaceticacid(BAPTA)의 영향을 조사하였다. 방 법 : 인체의 족삼리(足三里)(ST36)와 삼음교(三陰交)(SP6)에 해당하는 혈자리에 2 Hz전침 자극을 1.0mA 세기로 30분 동안 자극하였다. 결 과 : 전침 무통각을 측정한 결과 높은 농도의 BAPTA 복강내주사 처리군에서 저하가 관찰되었다. 전침 처치 후 60분 후 분리한 $L_{4-5}$ 척수 분절에서 C-fos 발현 신경세포 수는 BAPTA 처리에 의해 감소하는데 특히 고유핵에서 저하가 현저하였다. 평균 integrated optical density로 비교한 NR1과 NR2B subunit에 대한 면역조직화학적 발현을 보면 전침 자극은 정상군에 비해 얕은 층판에서 증가하였다. NR1과 NR2B subunit의 인산화형에 대한 발현을 보면 NR1 인산화형은 척수 배각 전 부위에서, NR2B 인산화형은 얕은 층판에서 증가하였으며 BAPTA 처리에 의해 NR1 인산화형은 얕은 층판과 목 부위에서 NR2B 인산화형은 얕은 층판에서 현저한 감소를 보였다. western blot로 살펴본 BAPTA 처리에 의한 NR1 및 NR2B 인산화형 변화는 면역조직화학적 방법과 유사한 결과를 보여 주었다. 결 론 : 전침 무통각은 세포내 칼슘에 의한 척수 내 NMDA 수용체의 인산화가 중요한 요인으로 작용할 가능성이 있다.

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