Effects of Root of Cibotii Rhizoma on Neuronal Damage of Spinal Cord Contusion Injury in Rats

구척(狗脊)이 흰쥐의 척수압박에 의한 신경세포 손상에 미치는 영향

  • Park, Won-Sang (Dept. of Oriental Rehabiiltation Medicine, Ja-Seng Hospital of Oriental Medicine) ;
  • Kim, Eun-Seok (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Shin, Jung-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kim, Bum-Hoi (Dept. of Anatomy, College of Oriental Medicine, Dong-Eui University) ;
  • Kim, Seong-Joon (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kang, Hee (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Sohn, Nak-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
  • 박원상 (자생한방병원) ;
  • 김은석 (경희대학교 동서의학대학원 한의과학전공) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학전공) ;
  • 김범회 (동의대학교 한의과대학 해부학교실) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학전공) ;
  • 강희 (경희대학교 동서의학대학원 한의과학전공) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학전공)
  • Received : 2010.02.02
  • Accepted : 2010.04.01
  • Published : 2010.04.30

Abstract

Objectives : This study was performed to evaluate the effects of root of Cibotii rhizoma(CR) ethanol extract on the tissue and neuronal damage of the spinal cord injury(SCI). Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. CR was orally given once a day for 7 days after SCI. Tissue damage and nerve fiber degeneration were examined with cresyl violet and luxol fast blue(LFS) histochemistry. HSP72(as neuronal damage marker), MAP2(as nerve fiber degeneration marker), c-Fos(immediate early gene), and Bax(pro-apoptotic molecule) expressions were examined using immuno-histochemistry. Individual immuno-positive cells expressing HSP72, MAP2, c-Fos and Bax were observed on the damaged level and the upper thoracic and lower lumbar spinal segments. Results : 1. CR reduced degeneration of nerve fibers and motor neuron shrinkage in the ventral horn of the lower lumbar spinal segment, but generally it did not seem to ameliorate the tissue injury following SCI. 2. CR reduced demyelination in the ventral and lateral funiculus of the lower lumbar spinal segment. 3. CR reduced HSP72 expression on the neurons in the peri-central canal gray matter adjacent to the damaged region. 4. CR strengthened MAP2 expression on the motor neurons in the ventral horn and on nerve fibers in the lateral funiculus of the lower lumbar spinal segment. 5. CR reduced c-Fos positive cells in the peri-lesion and the dorsal horn of the damaged level and in the ventral horn of the lower lumbar spinal segment. 6. CR reduced Bax positive cells in the peri-lesion and the dorsal horn of the damaged level and in the ventral horn of the lower lumbar spinal segment. Conclusions : These results suggest that CR plays an inhibitory role against secondary neuronal damage and nerve fiber degeneration. following SCI.

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