Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
권호 표지

Effects of Imyo-san Treatment on the Monosodium Iodoacetate-induced Osteoarthritis in Rats

이묘산(二妙散)이 흰쥐의 Monosodium Iodoacetate 유발 골관절염에 미치는 영향

  • Ahn, Hee-Bin (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Soon-Jooog (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Seo, Il-Bok (Dept. of Anatomy, College of Oriental Medicine, Se-Myung University) ;
  • Jeong, Su-Hyeon (Dept. of Oriental Rehabilitation Medicine, College of Oriental Medicine, Se-Myung University)
  • 안희빈 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 김순중 (세명대학교 한의과대학 한방재활의학과교실) ;
  • 서일복 (세명대학교 한의과대학 해부학교실) ;
  • 정수현 (세명대학교 한의과대학 한방재활의학과교실)
  • Received : 2010.06.15
  • Accepted : 2010.07.13
  • Published : 2010.07.30

Abstract

Objectives : This study was to investigate the effects of Imyo-san treatment on the monosodium iodoacetate-induced osteoarthritis in rats. Methods : Arthritis was induced by injection of monosodium iodoacetate(MIA)(0.5 mg) into both knee joint cavities of rats. Arthritic rats were divided into control(n=8) and treated(n=8) group. Control group was taken distilled water and treated group was taken extracts of Imyo-san by orally for 20 days. At the end of experiment(20day after MIA injection), gross and histopathological examination on the articular structures of knee joints were performed. Blood cell counts and proteoglycan(PG) contents in articular cartilages were analysed. And also, tumor necrosis factor-$\alpha$($TNF-{\alpha}$) and interleukin-$1{\beta}$($IL-1{\beta}$) contents synovial fluids were measured by enzyme-liked immunosorbent assay(ELISA) method. Results : 1. Body weight(g) of the treated group were increased significantly compared with control group at 15 and 20 days after injection. 2. Grossly, degree of osteoarthritis in the treated group was alleviated compared with the control group. 3. PG content in articular cartilage of the treated group was increased significantly compared with the control group. 4. Histopathologically, osteoarthritic scores of the treated group was decreased significantly compared with the control group. 5. $TNF-{\alpha}$ content in synovial fluid of the treated group was decreased significantly compared with the control group. Conclusions : On the basis of these results, we suggested that Imyo-san has inhibiting effects on the progression of arthritis in MIA-induced osteoarthritis model.

Keywords

References

  1. 한방재활의학과학회. 한방재활의학. 서울:군자출판사. 2006:83.
  2. 대한정형외과학회. 정형외과학. 제 6판. 서울:최신의학사. 2006:195-261, 517.
  3. Guccione AA, Felson DT, Anderson JJ, Anthony JM, Zhang Y, wilson PW, Kelly-Hayes M, Wolf PA, Kreger BE, Kannel WB. The effects of specific medical conditions on the functional limitations of elders in the Framingham study. Am J Public Health. 1994;84(3):351-8. https://doi.org/10.2105/AJPH.84.3.351
  4. 송영준, 하철원. 골관절염의 치료에서 COX-2 선택적 비스테로이드성 소염진통제의 이용. 대한슬관절학회지. 2009;21(2):84-92.
  5. 서승석, 김창완, 하동준, 최장석, 김호준, 이창락. 슬관절의 대퇴골과 골연골 결손에 대한 자가골연골 이식술. 대한정형외과학회지. 2009;44(3):301-10.
  6. Kuo AC, Rodrigo JJ, Reddi AH, Curtiss S, Grotkopp E, Chiu M. Microfracture and bone morphogenetic protein 7(BMP-7) synergistically stimulate articular cartilage repair. Osteoarthritis Cartilage. 2006;14:1126-35. https://doi.org/10.1016/j.joca.2006.04.004
  7. 김미려, 허수영, 박기현. 퇴행성 슬관절염에 대한 동서의학적 비교. 제한동의학술원논문집. 1999;4(1):711-22.
  8. 許浚. 東醫寶鑑. 서울:법인문화사. 2002:981.
  9. 김선모, 윤형정, 이현우, 김판중, 이창현, 박원환, 박선동. 이묘산에 의한 대장암 세포주 HCT116의 Caspases 활성화를 매게로 한 세포사멸. 대한방제학회지. 2006;14(2):21-32.
  10. Kong LD, Yang C, GE F, Wang HD, Guo YS. A Chinese herbal medicine Ermiao wan reduces serum uric acid level and inhibits liver xanthine dehydrogenase and xanthine oxidase in mice. J Ethnopharmacol. 2004;93(2-3):325-30. https://doi.org/10.1016/j.jep.2004.04.008
  11. 임창경. 이묘산의 항류마티즘 효능에 대한 연구. 대한한의학방제학회지. 2009;17(1):99-111.
  12. Janusz MJ, Hookfin EB, Heitmeyer SA, Woessner JF, Freemont AJ, Hoyland JA, Brown KK, Hsieh LC, Almstead NG, De B, Natchus MG, Pikul S, Taiwo YO. Moderation of iodoacetate-induced experimental osteoarthritis in rats by matrix metalloproteinase inhibitors. Osteoarthritis and Cartilage. 2001;9:751-60. https://doi.org/10.1053/joca.2001.0472
  13. Rudolphi K, Gerwin N, Verziji N, van der Kraan P, van den Berg W. Pralnacasan, an inhibitor of interleukin-1 $\beta$ converting enzyme, reduces joint damage in two murine models of osteoarthritis. Osteoarthritis and Cartilage. 2003;11(10):738-46. https://doi.org/10.1016/S1063-4584(03)00153-5
  14. Kasper 외. 대한내과학회 해리슨내과학 편집위원회. Harrison's 내과학. 서울:도서출판 MIP. 2006:2083-2084, 2225.
  15. 최승관, 정광환, 조성도, 고상훈, 차재룡, 이채칠, 염윤석, 박기봉. 골관절염에서 활막액 VEGF와 총 백혈구 수, 혈액 내 acute phase reactant 와의 상관관계. 대한정형외과연구학회지. 2008;11(2):100-4.
  16. 危亦林. 世醫得效方. 서울:의성당. 1990:320.
  17. 朱震亨. 丹溪心法. 북경시:인민출판위생출판사. 2001:255.
  18. 윤용갑. 동의방제와 처방해설. 서울:의성당. 2004:132.
  19. Guingamp C, Gegout-Pottie P, Philippe L, Terlain B, Netter P, Gillet P. Monosodium-iodoatetate-induced experimental osteoarthritis: a dose-response study of loss of mobility, morphology, and biochemistry. Arthritis Rheum. 1997;40(9):1670-9. https://doi.org/10.1002/art.1780400917
  20. Guzman RE, Evans MG, Bove S, Morenko B, Kilgore K. Monosodium-iodoacetate-induced histologic changes in subchondral bone and articular cartilage of rat femorotibial joints: an animal model of osteoarthritis. Toxicol Pathol. 2003;31(6):619-24. https://doi.org/10.1080/01926230390241800
  21. Kalbhen DA. Chemical model of osteoarthritis-a pharmacological evaluation. J Rheumatol. 1987;14:130-1.
  22. van Osch GJ, van der Kraan PM, van den Berg WB. Site-specific cartilage changes in murine degenerative knee joint disease induced by iodoacetate and collagenase. J Orthop Res. 1994;12(2):168-75. https://doi.org/10.1002/jor.1100120204
  23. Geilen CC, Weiprecht M, Orfanos CE. The mitogen-activated protein kinases system (MAP kinase cascade): its role in skin single transduction. J Dermatol Sci. 1996;12(3):255-62. https://doi.org/10.1016/0923-1811(95)00481-5
  24. Ehrlich MG, Mankin HJ, Jones H, Grossman A, Crispen C, Ancona D. Biochemical confirmation of an experimental osteoarthritis model. J Bone Joint Surg Am. 1975;57:392-6. https://doi.org/10.2106/00004623-197557030-00018
  25. Kikuchi T, Sakuta T, Yamaguchi T. Intra-articular injection of collagenase induces experimental osteoarthritis in mature rabbits. Osteoarthritis and Cartilage. 1998;6:177-86. https://doi.org/10.1053/joca.1998.0110
  26. Murat N, Karadam B, Ozkal S, Karatosun V, Gidener S. Quantification of papain-induced rat osteoarthritis in relation to time with the Makin score. Acta Orthop Traumatol Turc. 2007;41(3):233-7.
  27. 이귀녕, 권오현. 임상병리파일. 제 3판. 서울:의학문학사. 2003:718, 1060.
  28. Mary KC, Shawn OF. 곽한식, 김재원, 김하근, 정혜신, 태건식 역. 생화학. 제 5판. 서울:라이프사이언스. 2007:451.
  29. 김재도. 관절 연골의 Proteoglycan 생성에 있어서 양이온제가 미치는 영향. 고려대학교대학원. 1990.
  30. Malemud CJ. Fundamental pathways in osteoarthritis: An overview. Front biosci. 1999;4:659-61. https://doi.org/10.2741/malemud
  31. 강재성, 김광혁, 김문규, 김희선, 박문향, 박세광 외. 세포분자면역학. 서울:범문사. 2004:247-69, 306, 325, 494-7.
  32. Abbas AK, Lichtman AH. 세포분자면역학. 서울:범문사. 2004:243-88.
  33. Smolen JS, Aletaha D, Koeller M, Weisman MH, Emery P. New therapies for treatment on rheumatoid arthritis. Lancet. 2007;370(9602):1861-74. https://doi.org/10.1016/S0140-6736(07)60784-3