Effects of Hwangryunhaedok-tang(Huanglianjiedu-tang) on Locomotor Dysfunction of Contusive Spinal Cord Injury-induced Rats

황련해독탕(黃連解毒湯)이 척수 압박손상 흰쥐의 운동기능 장애에 미치는 영향

  • Seong, Ju-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kim, Ki-Yuk (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Bahn, Hyo-Jung (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Shin, Jung-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kang, Hee (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Kim, Seong-Joon (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University) ;
  • Sohn, Nak-Won (Division of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University)
  • 성주원 (경희대학교 동서의학대학원 한의과학전공) ;
  • 김기역 (경희대학교 동서의학대학원 한의과학전공) ;
  • 반효정 (경희대학교 동서의학대학원 한의과학전공) ;
  • 신정원 (경희대학교 동서의학대학원 한의과학전공) ;
  • 강희 (경희대학교 동서의학대학원 한의과학전공) ;
  • 김성준 (경희대학교 동서의학대학원 한의과학전공) ;
  • 손낙원 (경희대학교 동서의학대학원 한의과학전공)
  • Received : 2010.09.19
  • Accepted : 2010.10.12
  • Published : 2010.10.30

Abstract

Objectives : This study was performed to evaluate the effects of Hwangryunhaedok-tang(Huanglianjiedu-tang HHT) water extract on locomotor dysfunction induced by spinal cord injury(SCI) in rats. Methods : SCI was induced by mechanical contusion following laminectomy of 10th thoracic vertebra in Sprague-Dawley rats. HHT was orally given once a day for 14 days after SCI. Neurological behavior was examined with the Basso-Beattie-Bresnahan locomotor rating scale. Tissue damage and nerve fiber degeneration were examined with cresyl violet and luxol fast blue staining. Using immunohistochemisty, cellular damage to neurons and nerve fibers were examined against Bax and MAP-2. As inflammatory response markers, iNOS and COX-2 expressions were also examined. Results : 1. HHT ameliorated the locomotor dysfunction of the SCI-induced rats. 2. HHT attenuated the reduction of motor neurons in the ventral horn of the SCI-induced rat spinal cord. 3. HHT significantly reduced the number of Bax positive cells in the peri-lesion of the SCI-induced rat spinal cord. 4. HHT attenuated the reduction of MAP-2 positive cells in the peri-lesion of the SCI-induced rat spinal cord. 5. HHT significantly reduced the number of iNOS and COX-2 positive cells in the peri-lesion of the SCI-induced rat spinal cord. Conclusions : These results suggest that HHT improves the locomotor dysfunction of SCI by protecting motor neurons from cell death through anti-inflammatory effect.

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