Changes in Nutritive Value and Digestion Kinetics of Canola Seed Due to Microwave Irradiation

  • Ebrahimi, S.R. (Department of Animal Science, Faculty of Agriculture, Shahr-e-Qods Branch, Islamic Azad University) ;
  • Nikkhah, A. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch, Islamic Azad University) ;
  • Sadeghi, A.A. (Department of Animal Science, Faculty of Agriculture, Science and Research Branch, Islamic Azad University)
  • Received : 2008.10.08
  • Accepted : 2009.02.02
  • Published : 2010.03.01


This study aimed to evaluate effects of 800 W microwave irradiation for 2, 4 and 6 min on chemical composition, antinutritional factors, ruminal dry matter (DM) and crude protein (CP) degradability, and in vitro CP digestibility of canola seed (CS). Nylon bags of untreated or irradiated CS were suspended in the rumen of three bulls from 0 to 48 h. Protein subfractions of untreated and microwave irradiated CS before and after incubation in the rumen were monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Microwave irradiation had no effect on chemical composition of CS (p>0.05). There was a linear decrease (p<0.001) in the phytic acid and glucosinolate contents of CS as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased the phytic acid content of CS by 8.2, 27.6 and 48.6%, respectively. The total glucosinolate contents of CS microwave irradiated for 2, 4 and 6 min decreased by 41.5, 54.7 and 59.0% respectively, compared to untreated samples. The washout fractions of DM and CP and degradation rate of the b fraction of CP decreased linearly (p<0.001) as irradiation time increased. Microwave irradiation for 2, 4 and 6 min decreased effective degradability (ED) of CP at a ruminal outflow rate of 0.05 $h^{-1}$ by 4.7, 12.3 and 21.0%, respectively. Microwave irradiation increased linearly (p<0.001) in vitro CP digestibility of ruminally undegraded CS collected after 16 h incubation. Electrophoresis results showed that napin subunits of untreated CS disappeared completely within the zero incubation period, whereas cruciferin subunits were degraded in the middle of the incubation period (16 h incubation period). In 4 and 6 min microwave irradiated CS, napin subunits were degraded after 4 and 16 h incubation periods, respectively, and cruciferin subunits were not degraded untile 24 h of incubation. In conclusion, it seems that microwave irradiation not only protected CP of CS from ruminal degradation, but also increased in vitro digestibility of CP. Moreover, microwave irradiation was effective in reducing glucosinolate and phytic acid contents of CS.


  1. AOAC. 1995. Official methods of analysis, 16th ed. Association of Official Analytical Chemists. Arlington, VA, USA
  2. Deacon, M. A., G. de Boer and J. J. Kennelly. 1988. Influence of jet-sploding and extrusion on ruminal and intestinal disappearance of canola and soybeans. J. Dairy Sci. 71:745-753
  3. Dong, Z., R. Long, D. Zhang, Z. Hu and X. Pu. 2005. Effect of microwave treatment on chemical composition and in sacco digestibility of wheat straw in Yak cow. Asian-Aust. J. Anim. Sci. 18:27-31
  4. Duodu, K. G., A. Minnaar and J. R. N. Taylor. 1999. Eeffct of cooking and irradiation on the labile vitamins and antinutrient content of a traditional African sorghum porridge and spinach relish. Food Chem. 66:21-27
  5. Englard, S. and S. Seifter. 1990. Precipitation techinques. Methods Enzymol. 182:285-300
  6. Fakhouri, M. O. and H. S. Ramaswamy. 1993. Temperature uniformity of microwave heated food as influenced byproduct type and composition. Food Res. Inter. 26:89-95
  7. Guan, J. J., A. Y. Qui, X. Y. Liu,Y. F. Yua and Y. H. Ma. 2006. Microwave improvement soy protein isolate-saccharide graft reactions. Food Chem. 97:577-585
  8. Hurrell, R. F. and K. J. Carpenter. 1977. Nutritional significance of crosslink formation during food processing. In: Protein crosslinking. Nutritional and medical consequences (Ed. M. Friedman). Plenum Press, New York, NY, USA, pp. 225-238
  9. Khatoon, N. and J. Prakash. 2004. Nutritional quality of microwave-cooked and pressure-cooked legumes. Int. J. Food Sci. Nutr. 55:441-448
  10. Lee Maire, M., L. Thauvette, B. De Foresta, A. Viel, G. Beauregard and M. Potier. 1990. Effects of ionizing radiations on proteins. Biochem. J. 267:431-439
  11. Mubarak, A. E. 2005. Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem. 89:489-495
  12. National Research Council 1996. Nutrient requirements of beef cattle. 7th Ed. National Academy Press, Washington, DC
  13. Sadeghi,, A. A. and P. Shawrang. 2007. Effects of microwave irradiation on ruminal protein degradation and intestinal digestibility of cottonseed meal. Livest. Sci. 106:176-181
  14. Taghinejad, M., A. Nikkhah, A. A. Sadeghi, G. Raisali and M. Chamani. 2009. Effects of gamma irradiation on chemical composition, antinutritional factors, ruminal degradation and in vitro protein digestibility of full-fat soybean. Asian-Aust. J. Anim. Sci. 22:534-541
  15. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597
  16. Wang, Y., T. A. McAllister, M. D. Pickard, Z. Xu, L. M. Rode and K. J. Chen. 1999. Effect of micronizing full fat canola seed on amino acid disappearance in the gastrointestinal tract of dairy cows. J. Dairy Sci. 82:537-544
  17. Yu, P., S. Tamminga, A. R. Egan and D. A. Christensen. 2004. Probing equivocal effects of heat processing of legume seeds on performance of ruminants - A review. Asian-Aust. J. Anim. Sci. 17:869-876
  18. Chen, Q. C. and W. L. Betty. 2003. Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications. J. Chromatogr. A. 1018:41-52
  19. Murray, R. K., D. K. Granner, P. A. Mayes and V. W. Rodwell. 2003. Harper's Biochemistry, 26th ed. McGraw-Hill, New York, NY, USA
  20. Broderick, G. A., R. J. Wallce and E. R. Orskov. 1991. Control of rate and extent of protein degradation. In: Physiological aspects of digestion and metabolism in ruminants (T. Tsuda, Y. Sasaki and R. Kawashima). Academic Press, San Diego CA, USA, pp. 542-592
  21. Debnath, D., N. P. Sahu, A. K. Pal, K. Baruah, S. Yengkokpam and S. C. Mukherjee. 2005. Present scenario and future prospects of phytase in aquafeed-review. Asian-Aust. J. Anim. Sci. 18:1800-1812
  22. PrestlOkken, E. 1999. Ruminal degradability and intestinal digestibility of protein and amino acids in barley and oats expander-treated at various intensities. Anim. Feed Sci. Technol. 82:157-175
  23. Shawrang, P., A. Nikkhah, A. Zare-Shahneh, A. A. Sadeghi, G. Raisali and M. Moradi-Shahrebabak. 2008. Effects of $\gamma$-irradiation on chemical composition and ruminal protein degradation of canola meal. Radiat. Phys. Chem. 77:918-922
  24. Gonzalez, J., J. Faria-Marmol, B. Matesanz, C. A. Rodriguez and M. R. Alvir. 2003. In situ intestinal digestibility of dry matter and crude protein of cereal grains and rapeseed in sheep. Reprod. Nutr. Dev. 43:29-40
  25. Banik, S., S. Bandyopadhyay and S. Ganguly. 2003. Bioeffects of microwave-a brief review. Bioresour. Technol. 87:155-159
  26. Fathi Nasri, M. H., J. France, M. Danesh Mesgaran and E. Kebreab. 2008. Effect of heat processing on ruminal degradability and intestinal disappearance of nitrogen and amino acids in Iranian whole soybean. Livest. Sci. 113:43-51
  27. Gharaghani, H., M. Zaghri, G. Shahhosseini and H. Moravej. 2008. Effect of gamma irradiation on antinutritional factors and nutrition value of canola meal for broiler chickens. Asian-Aust. J. Anim. Sci. 21:1479-1485
  28. Negi, A., P. Boora and N. Khetarpaul. 2001. Effect of microwave cooking on the starch and protein digestibility of some newly released moth bean (Phaseolus aconitifolius Jacq.) cultivars. J. Food Compo. Anal. 14:541-546
  29. Oerlemans, K., D. M. Barrett, C. B. Suades, R. Verkerk and M. Dekker. 2006. Thermal degradation of glucosinolates in red cabbage Food Chem. 95:19-29
  30. Moshtaghi Nia, S. A. and J. R. Ingalls. 1992. Effect of heating on canola meal protein degradation in the rumen and digestion in the lower gastrointestinal tract of steers. Can. J. Anim. Sci. 72:83-88
  31. Azarfar, A., C. S. Ferreira. J. O. Goelema and A. F. B. Van Der Poel. 2008. Effect of pressure toasting on in situ degradability and intestinal protein and protein-free organic matter digestibility of rapeseed. J. Sci. Food Agric. 88:1380-1384
  32. Maheshwari, P. N., D. W. Stanley and F. R. Van de Van. 1980. Microwave treatment of dehulled rapeseed meal to inactive myrosinse and its effect on oil meal quality. J. Am. Oil Chem. Soc. 57:194-199
  33. Clifford, A. and D. V. Smith. 2006. Rapid method for determinig total glucosinolates in rapeseed by enzimatically released glucose. J. Sci. Food Agric. 38:141-150
  34. Orskov, E. R. and I. McDonaled. 1979. The estimation of protein degradability in rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92:499-503
  35. Sarıçiçek, B. Z., U. Kılıç and A. V. Garipoğlu. 2005. Replacing soybean meal (SBM) by canola meal (CM): The effects of multi-enzyme and phytase supplementation on the performance of growing and laying quails. Asian-Aust. J. Anim. Sci. 18:1457-1463
  36. Voragen, A. G. J., H. Gruppen, G. J. P. Marsman and A. J. Mul. 1995. Effect of some manufacturing technologies on chemical, physical and nutritional properties of feed. In: Recent advances in animal nutrition (Ed. P. C. Gransworthy and D. J. A. Cole), Nottingham university press, Nottingham, UK, pp. 93-126
  37. De Boland, A. R., G. B. Garner and B. L. O Dell. 1975. Identification and properties of phytate in cereal grains and oil seed products. J. Agric. Food Chem. 23:1186-1189
  38. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
  39. Madsen, J. and P. G. Hvesplund. 1985. Protein degradation in the rumen. A comparison between in vivo, nylon bag, in vitro and buffer measurements. Acta Agric. Scand. 25:103-124
  40. Huang, S., M. Liang, G. Lardy, H. E. Huff, M. S. Kerley and F. Hsieh. 1995. Extrusion process of rapeseed meal for reducing glucosinolates. Anim. Feed Sci. Technol. 56:1-9
  41. Mustafa, A. F., J. J. McKinnon and D. A. Christensen. 2000. Protection of canola (Low glucosinolate rapeseed) meal and seed protein from ruminal degradation - Review. Asian-Aust. J. Anim. Sci. 13:535-542
  42. Oliveira, M. E. C. and A. S. Franca. 2002. Microwave heating of foodstuffs. J. Food Eng. 53:347-359
  43. Fenwick, G. R., E. A. Spinks, A. P. Wilkinson, R. K. Heaney and M. A. Legoy. 1986. Effect of processing on the antinutrient content of rapeseed. J. Sci. Food Agric. 37:735-741
  44. Finley, J. W. 1989. Effects of processing on proteins: an overview. In: Protein quality and the effects of processing (Ed. R. D. Phillips and J. W. Finley) Marcel Dekker, New York, NY, USA, pp. 1-7
  45. National Research Council 2001. Nutrient requirements of dairy cattle. 7th Ed. National Academy of Sciences, Washington, DC. USA
  46. Vallejo, F., F. A. Tomas-Barbern and C. Garcia-Viguera. 2002. Glucosinolate and vitamin C contents in edible parts of broccoli florets after domestic cooking. Eur. food Res. Technol. 215:310-316
  47. SAS Institute Inc. 1996. Statistical Analysis System (SAS) User's Guide, SAS Institute, Cary, NC, USA
  48. Sadeghi,, A. A. and P. Shawrang. 2006. Effects of microwave irradiation on ruminal degradability and in vitro digestibility of canola meal. Anim. Feed Sci. Technol. 127:45-54
  49. Alajaji, S. A. and T. A. El-Adawy. 2006. Nutritional composition of chickpea (Cicer arietinum L.) as affected by microwave cooking and other traditional cooking methods. J. Food Compo. Anal. 19:806-812
  50. Calsamiglia, S. and M. D. Stern. 1995. A three-step in vitro procedure for estimating intestinal digestion of protein in ruminants. J. Anim. Sci. 73:1459-1465
  51. Jensen, S. K., Y. G. Liu and B. O. Eggum. 1995. The effect of heat treatment on glucosinolates and nutritive value of rapeseed meal in rat. Anim. Feed Sci. Technol. 53:17-28
  52. Chen, P., P. Ji and S. L. Li. 2008. Effect of feeding extruded soybean, ground canola seed and whole cottonseed on ruminal fermentation, performance and milk fatty acid profile in early lactation dairy cows. Asian-Aust. J. Anim. Sci. 21:204-213

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