Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1

Zhi, Ai-min;Zhou, Xiang-yan;Zuo, Jian-jun;Zou, Shi-geng;Huang, Zhi-yi;Wang, Xiao-lan;Tao, Lin;Feng, Ding-yuan

  • Received : 2009.05.06
  • Accepted : 2009.07.20
  • Published : 2010.02.01


In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.


Cloning;Pig;Amino Acid Transporter;$y^{+}$LAT1;SLC7A7


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