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소매시장에서 판매하는 돼지고기와 닭고기에서 분리된 Escherichia coli의 병원성 인자

최선금;이민화;이복희;정지윤;최창순
Choi, Sun-Keum;Lee, Min-Hwa;Lee, Bog-Hieu;Jung, Ji-Youn;Choi, Chang-Sun

  • 발행 : 2010.02.28

Abstract

시중에서 판매되는 계육과 돈육 가검물 1085개를 구입하고, 총 217개의 대장균 분리주를 확보하였다. 현재 알려진 대장균 병원성 인자 11개(astA, elt, estI, estII, cdt, cnf, eae, afa, stx, inve, aggR)의 보유여부를 PCR 기법으로 조사하였다. 돈육유래 대장균에서 존재하는 병원성 인자는 astA(6.9%), eae와 elt(4.6%), estII(4.0%), estI(2.3%), afa (1.1%), cnf(0.6%)의 순으로 검출되었으나, cdt, stx, aggR, inve는 검출되지 않았다. 계육에서 분리된 대장균 42개 중에서 astA와 inve를 보유하고 있는 병원성 대장균이 각각 1개씩 확인되었다. 특히 astA는 돈육과 계육에서 분리된 대장균 중에서 높은 빈도로 존재하고 있음을 확인하였으며, cnf와 afa같이 기존에 알려지지 않은 병원성 인자들의 존재도 확인되었다.

Keywords

Escherichia coli;astA;prevalence;pork;chicken

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