- Volume 36 Issue 3
Biological degradation of nitrogen-containing aromatic compounds was investigated in activated sludge previously adapted to mineralize low concentrations of nitrogen-containing aromatic compounds. Normally, the time required for 95% degradation of 10 mg/l dinitrophenol (DNP) under aerobic conditions was less than 4 hours without any lag, and with mixed liquor suspended solid (MLSS) levels from 600 to 1,000 mg/l. However, when the initial DNP concentration was increased to 75 mg/l, lags and even complete inhibition of DNP degradation were observed. The length of the lag was found to increase proportionally with decreasing MLSS levels. When dilute activated sludge was incubated for extended periods (192 hours), degradation of 75 mg/l DNP did eventually occur after lag periods of 37 to 144 hours, depending on the MLSS concentration. DNP was degradable in high concentrations if MLSS concentrations were sufficiently high to allow growth of bacteria resistant to the toxic effects of DNP.
nitrogen-containing aromatic compound;nitrophenol;inhibition;activated sludge
- Liu, X., Wang, B., Jiang, C., Zhao, K., Harold, L. D. and Liu, S. J. : Simultaneous biodegradation of nitrogencontaining aromatic compounds in a sequencing batch bioreactor. Journal of Environmental Science, 19, 530-535, 2007. https://doi.org/10.1016/S1001-0742(07)60088-6
- Song, H. I. and Kim, J. O. : Characterization of biological treatment by an isolated phenol-degrading bacterium. Korean Journal of Environmental Health Sciences, 24, 54-62, 1998.
- Hallas, L. E. and Alexander, M. : Microbial transformation of nitroaromatic compounds in sewage effluent. Applied and Environmental Microbiology, 45, 1234- 1241, 1983.
- Okey, R. W. and Stensel, H. D. : Uncouplers and activated sludge - The impact on synthesis and respiration. Toxicology and Environmental Chemistry, 40, 235-254, 1993. https://doi.org/10.1080/02772249309357946
- Lenke, S., Pieper, D. H., Bruhn, C. and Knackmuss, H.-J. : Degradation of 2,4-dinitrophenol by two Rhodococcus erythropolis strains, HL 24-1 and HL 24-2. Applied and Environmental Microbiology, 58, 2928-2932, 1992.
- Hess, T. F., Silverstein, J. and Schmidt, S. K. : Effect of glucose on 2,4-dinitrophenol degradation kinetics in sequencing batch reactors. Water Environment Research, 65, 73-81, 1993. https://doi.org/10.2175/WER.65.1.10
- Singirtsev, I. N., Krest'yaninov, V. Y. and Korzhenevich, V. I. : Biological degradation of 2,4-dinitrophenol. Applied and Biochemical Microbiology, 30, 204-207, 1994.
- Tokuz, R. Y. : Response of phthalate esters-acclimated activated sludge to 2,4-dinitrophenol. Water Science and Technology, 19, 461-470, 1987.
- Kincannon, D. F., Stover, E. L., Nichols, V. and Medley, D. : Removal mechanisms for toxic priority pollutants. Journal of Water Pollution Control Federation, 55, 157-163, 1983.
- Wiggins, B. A. and Alexander, M. : Role of chemical concentration and second carbon sources in acclimation of microbial communities for biodegradation. Applied and Environmental Microbiology, 54, 2803-2807, 1988.
- Ettala, M., Koskela, J. and Kiesila, A. : Removal of chlorophenols in a municipal sewage treatment plant using activated sludge. Water Research, 26, 797-804, 1992. https://doi.org/10.1016/0043-1354(92)90011-R
- Chudoba, J., Albokova, J. and Cech, J. S. : Determination of kinetic constants of activated sludge microorganisms responsible for degradation of xenobiotics. Water Research, 23, 1431-1438, 1989. https://doi.org/10.1016/0043-1354(89)90083-3
- Hess, T. F., Schmidt, S. K., Silverstein, J. and Howe, B. : Supplemental substrate enhancement of 2,4-dinitrophenol mineralization by a bacterial consortium. Applied and Environmental Microbiology, 56, 1551-1558, 1990.
- Kim, T. J. : The degradation of phenolic compounds by Lignolytic Streptomyces strains. Korean Journal of Environmental Health Sciences, 26, 86-91, 2000.
- Am. Water Works Assoc., Water Environ. Fed., Am. Public Health Assoc. : Standard methods for the examination of water and wastewater. 21st ed., Washington, D. C., 2005.