Antioxidative and Antigenotoxic Effect of Omija (Schizandra chinensis B.) Extracted with Various Solvents

다양한 용매에 의해 추출된 오미자의 항산화능과 항유전독성 효과

  • Kim, Min-Jung (Dept. of Food and Nutrition, Kyungnam University) ;
  • Park, Eun-Ju (Dept. of Food and Nutrition, Kyungnam University)
  • 김민정 (경남대학교 식품영양학과) ;
  • 박은주 (경남대학교 식품영양학과)
  • Published : 2010.04.30


The purpose of this study was to evaluate antioxidant and antigenotoxic effects of Omija (Schizandra chinensis B.) extracted with various solvents (acetone, ethanol, and methanol). The total polyphenol content (TPC) of methanol extract (ME), ethanol extract (EE) and acetone extract (AE) from Omija were 1183.3, 1009.4, and 747.3 mg/100 g (garlic acid equivalents: GAE), respectively. Antioxidant effects of the Omija extracts was measured by DPPH radical-scavenging activity (RSA) and superoxide dismutase (SOD)-like activity. The $IC_{50}$ for DPPH RSA was in the order of EE $(1411.1\;{\mu}g/mL)$=AE $(1462.0\;{\mu}g/mL)$>ME $(1585.0\;{\mu}g/mL)$. The $IC_{50}$ for SOD-like activities was the highest in ME $(905.7\;{\mu}g/mL)$=EE $(970.3\;{\mu}g/mL)$>AE $(1579.4\;{\mu}g/mL)$. The antigenotoxic effect of Omija on DNA damage induced by $H_2O_2$ in human leukocytes was evaluated by comet assay. $H_2O_2$ induced DNA damage was effectively protected by all of the Omija extracts. Aectone extract of Omija showed the highest antigenotoxic effect ($IC_{50}$ value of AE is $14.6\;{\mu}g/mL$) followed by EE, and ME (21.4 and $34.4\;{\mu}g/mL$), respectively. As a result, we propose that Omija (Schizandra chinensis B.) can serve as a new natural source enriched with potent antioxidant and antigenotoxic agents.


  1. Gutteridge JMC, Halliwell B. 1994. Antioxidants in nutrition, health, and disease. Oxford University Press, Oxford, UK. p 1-62.
  2. Park MH, Kang SM, Jung HY, Hong SG. 2003. Protecting effects of vitamine E against immobilization stress-induced oxidative damage in rat brain. Korean J Nutr 36: 570-576.
  3. Wiseman H. 1996. Dietary influences on membrane function: importance in protection against oxidative damage and disease. J Nutr Biochem 7: 2-15.
  4. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84.
  5. Ji LL. 1993. Antioxidant enzyme response to exercise and aging. Med Sci Sport Exercise 25: 225-231.
  6. Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59: 527-605.
  7. Wickens AP. 2001. Ageing and the free radical theory.Respir Physio 128: 379-391.
  8. Kinsella JE, Frankel E, German B, Kanner J. 1993. Possible mechanisms for the protective role of antioxidants in wine and plant foods. Food Technol 47: 85-89.
  9. Lee JS, Lee MG, Lee SW. 1989. A study on the general components and minerals in parts of Omija (Schizandra chinensis Baillon). Kor J Dietary Culture 4: 173-176.
  10. Lee JS, Lee SW. 1990. Effect of water extracts in Omija (Schizndra chinenesis Baillon) on alcohol metabolism. Kor J Dietary Culture 5: 259-263.
  11. 이춘영, 김우정. 1987. 천연향신료와 식용색소. 향문사, 서울. p 95.
  12. Kim DG, Kim MB, Kim H, Park JH, Im JP, Hong SH. 2005. Herb medicinal pharmacognosy. Shinill Books, Seoul, Korea. p 407.
  13. Mok CK. 2005. Quality characteristics of instant tea prepared from spray-dried Omija (Schizandra chinensis Baillon) extract/grape juice mixture. Food Eng Prog 9: 226-230.
  14. Oh SL, Kim SS, Min BY, Chung DH. 1990 Composition of free sugars, free amino acid, non-volatile organic acids and tannins in the extracts of L. chinesis M., A. acutiloba K., S. chinensis B. and A. sessiliflorum S. Korean J Food Sci Technol 22: 76-81.
  15. Kim KI, Nam JH, Kwon TW. 1973. On the peroximate composition, organic acid and anthocyanins of Omija (Schizandra chinensis Baillon). Korean J Food Sci Technol 5: 178-182.
  16. Yang HC, Lee JM, Song KB. 1982. Anthocyanins in cultured Omija (Schizandra chinensis Baillon) and its stability. J Korean Soc Agri Chem Biotechnol 25: 35-43.
  17. Lee JS, Lee SW. 1989. A study on the compositions of free sugars, lipids, and nonvolatile organic acids in parts of omija (Schizandra chinensis Baillon). Korean J Dietary Culture 4: 177-181.
  18. Lee JS, Lee SW. 1990. Effect of water extract in fruits of Omija (Schizandra chinensis Baillon) on alcohol metabolism. Korean J Dietary Culture 5: 259-262.
  19. Sheo HJ, Lee MY, Hwang JS. 1987. Effect of Schizandrae fructus extract on blood constituents of alloxan induced diabetic rabbits. J Korean Soc Food Sci Nutr 16: 262-268.
  20. Kim JS, Choi SY. 2008. Physicochemical properties and antioxidativeactivities of Omija (Schizandra chinensis Baillon).Korean J Food & Nutr 21: 35-42.
  21. Jeon YH, Kil JH, Lim SM, Kim MH, Kim MR. 2008. Analysis of antioxdative activity and antimutagenic effect of ethanol extract from Schizandra chinensis Baillon. J East Asian Soc Dietary Life 18: 746-752.
  22. Chen N, Chiu PY, Ko KM. 2008. Schisandrin B enhances cerebral mitochondrial antioxidant status and structural integrity, and protect against cerebral ischemia/reperfusion injury in rats. Biol Pharm Bull 31: 1387-1391.
  23. Chiu PY, Leung HY, Ko KM. 2008. Schisandrin B enhances renal mitochondrial antioxidant status, functional and structural integrity, and protects against gentamicin-induced nephrotoxicity in rats. Biol Pharm Bull 31: 602-605.
  24. Jang EH, Pyo YH, Ahn MS. 1996. Antioxidant effect of Omija (Schizandra chinensis Baillon) extracts. Korean J Soc Food Sci 12: 372-376.
  25. Folin O, Denis W. 1912. On phosphotungasticphosphomolybdic compounds as color reagents. J Biol Chem 12: 239-249.
  26. Mensor LL, Menezes FS, Leitao GG, Reis AS, Santos TC, Coube CS, Leitao SG. 2001. Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 15: 127-130.
  27. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Bidchem 47: 468-474.
  28. Singh PN, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191.
  29. Cho YJ, Ju IS, Kim BC, Lee WS, Kim MJ. 2007. Biological activity of Omija (Schizandra chinensis Baillon) extracts.J Korean Soc Appl Biol Chem 50: 198-203.
  30. Kim SI, Sim KH, Ju SY, Han YS. 2009. A study of antioxidative and hyperglycemic activities of Omija (Schizandra chinensis Baillon) extract under variable extract conditions. Korean J Food & Nutr 22: 41-47.
  31. Kim HB, Kim SK, Moon JY, Chang SJ. 2003. Quantification and varietal variation of free sugars in mulberry fruits. Kor J Seric Sci 45: 80-84.
  32. Kim CJ, Suh HJ. 2005. Antioxidant activities of Rhubarb extracts containing phenolic compounds. Korean J Food Culture 20: 77-85.
  33. Huang D, Ou B, Prior RL. 2005. The chemistry behind antioxidant capacity assays. J Agric Food Chem 53: 1841-1856.
  34. Shimada KK, Fujikawa KY, Nakamura T. 1992. Antioxidative properties of xanthan on autoxidation of soybean oil in cyclodextrin. J Agric Food Chem 40: 945-948.
  35. Song JH, Lee HS, Hwang JK, Chang TY, Hong SR, Park KM. 2003. Physiological activities of Phellinus ribis extracts. Korean J Food Sci Technol 35: 690-695.
  36. Ostling O, Johanson KJ. 1984. Microgel electrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123:291-298.
  37. Collins AR, Gedik CM, Olmedilla B, Southon S, Bellizzi M. 1998. Oxidative DNA damage measured in human lymphocytes: large differences between sexes and between countries, and correlation with heart disease mortality rates. FASEB J 12: 1397-1400.
  38. Donald MW, Nell GH. 1984. Biological activities of lignans.J Phytochem 23: 1207-1213.

Cited by

  1. Antioxidant and α-glucosidase inhibitory activities of different solvent extracts of skullcap (Scutellaria baicalensis) vol.20, pp.4, 2011,
  2. Effect of Dendranthema indicum Extracts on Cell and DNA Damage Induced by Oxidative Stress vol.21, pp.12, 2011,
  3. Antimicrobial Activity of Psoralea corylifolia, Schisandra chinensis, and Spatholobus suberectus Extracts vol.45, pp.4, 2013,
  4. Dehydration of Omija (Schisandra chinensis B.) using Red Algae Extract as a Hypertonic Agent vol.20, pp.2, 2013,
  5. Quality Characteristics and Antioxidant Activity of Makgeolli Supplemented with Omija Berries (Schizandra chinensis Baillon) vol.47, pp.3, 2015,
  6. Consumer Awareness on Omija using Qualitative Consumer Research     vol.25, pp.3, 2015,
  7. Evaluation of the Quality Characteristics in Omija (Schizandra chinensis Baillon) Powder Tea vol.48, pp.1, 2016,
  8. Antioxidation activity of residue after omija (Schisandra chinensis) juice extract vol.60, pp.2, 2017,
  9. The Comparative Study on Anti-inflammatory Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Ligustici Rhizoma vol.14, pp.1, 2011,
  10. Effects of drying methods on quality characteristics and antioxidative effects of Omija (Schizandra chinesis bailon) vol.21, pp.3, 2014,