PCR-based identification of uncultural bacterium from malformed Agaricus blazei

신령버섯의 기형으로부터 배양불능세균의 PCR 검정

  • Shin, Pyung-Gyun (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Park, Yun-Jung (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Yoo, Young-Bok (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Oh, Se-Jong (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Lee, Keum-Hee (National Plant Quarantine Service, MIFAFF)
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 박윤정 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오세종 (국립농업과학원 유전자원센터) ;
  • 이금희 (농수산식품부 국립식물검역원)
  • Received : 2010.11.29
  • Accepted : 2010.12.27
  • Published : 2010.12.31

Abstract

Agaricus blazei Murill is a important medicinal mushroom for a powerful immune system builder and tonic. Currently, it is known about a new disease phenomenon that appears to be occurring on a number of mushroom farms. We described a straightforward approach in which molecular methods was used to survey the presence of potentially endo- and epiphytic bacteria infected with the Agaricus blazei. The 16S rDNA was amplified with universal eubacterial primers directly from pure cultures of Agaricus blazei mycelium and fruit body. The 16S rDNA sequences were almost identical (96 to 97% similarity), and phylogenetic analysis placed them in a single unique rRNA branch belong to the uncultural bacterium phylogroup. PCR detection of uncultural bacterium in the malformed tissues of Agaricus blazei were carried out by using 16S rRNA sequenced specific probe. It was strongly amplified at the malformed pileus region of fruit body and also spore print was impossible.