Isolation of neohaplonts and application of spo11 marker to identify sporeless Pleurotus ostreatus

무포자느타리 선발을 위한 단핵화 균주의 분리 및 Spo11 마커의 이용

  • Shin, Pyung-Gyun (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Yoo, Young-Bok (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Oh, Se-Jong (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Park, Yun-Jung (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Kong, Won-Sik (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Jang, Kab-Yeul (Mushroom Science Division, National Institute of Horticultural and Herbal Science) ;
  • Lee, Keum-Hee (National Plant Quarantine Service, MIFAFF)
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 유영복 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 오세종 (국립농업과학원 유전자원센터) ;
  • 박윤정 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 공원식 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 장갑열 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 이금희 (농수산식품부 국립식물검역원)
  • Received : 2010.12.13
  • Accepted : 2010.12.24
  • Published : 2010.12.31

Abstract

For the development of a sporeless strain of P. ostreatus we used sporeless strain ASI 2069. We have recovered both nuclear types of strain ASI 2069 as monokaryons of nh9, nh15, nh26 and nh36 (here after referred to as neohaplonts) by protoplasting the mycelium. Crosses between neohaplonts and SSI's(single spore isolates) obtained from a sporulating commercial strain ASI 2180. Five excellent strains are selected from 30 bred strains by quality of fruitbodies and spore number. To development of molecular markers linked to sporeless strain of P. ostreatus, we are screened helicase, recombinase(DMC1) and topoisomerase(Spo11) genes related meiosis by PCR and sequencing. Among three genes, Spo11 gene was identified into molecular marker of sporeless from neohaplonts and bred strains of P. ostreatus.