Phylogenetic relationships of medicinal mushroom Sparassis crispa strains using the rDNA-ITS and CAPS analysis

rDNA-ITS 및 CAPS 분석에 의한 꽃송이버섯 (Sparassis crispa) 수집균주의 계통분류학적 특성구분

  • Cheong, Jong-Chun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Myung-Chul (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Jhune, Chang-Sung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Chan-Jung (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Shin, Pyeong-Gyun (Mushroom Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 정종천 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 이명철 (국립농업과학원 농업유전자원센터) ;
  • 전창성 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 이찬중 (농촌진흥청 국립원예특작과학원 버섯과) ;
  • 신평균 (농촌진흥청 국립원예특작과학원 버섯과)
  • Received : 2010.02.18
  • Accepted : 2010.03.08
  • Published : 2010.03.31

Abstract

This study was carried out to analyze the genetic relationships among 22 strains of Sparassis crispa, which were collected from various regions of worldwide. The cleaved amplified polymorphic sequence were obtained from the ribosomal DNA ITS regions of each strain. Based on the sequence analysis, the presence of five different groups were observed. Most strains shared the high nucleotide sequence similarity (about 90%) to each other, except only one strain, KACC50866. Nucleotide sequence similarity of KACC50866 was below 10% to other strains, indicating the genetic relatedness of strain KACC50866 was low compared to other strains. More works such as mitochondria genome analysis should help to determine the precise genetic diversity of S. crispa strains.

Keywords

PCR;RAPD;ribosomal DNA;Sequencing;Sparassis crispa