Fabrication of Porous Calcium Phosphate by Using a Pre-Form of Nature Material

자연물의 미세구조를 활용한 다공성 인산칼슘 제조

  • Lee, Sang-Jin (Department of Advanced Materials Science and Engineering, Mokpo National University) ;
  • Lee, Hoon-Cheol (Department of Advanced Materials Science and Engineering, Mokpo National University)
  • 이상진 (국립목포대학교 신소재공학과) ;
  • 이훈철 (국립목포대학교 신소재공학과)
  • Received : 2010.03.19
  • Accepted : 2010.05.10
  • Published : 2010.05.31


Porous calcium phosphates were successfully fabricated by using a cuttlefish bone. The cuttlefish bone, which is composed of $CaCO_3$, showed a special porous microstructure containing uniform-sized voids. In this study, the pre-forms infiltrated distilled phosphoric acid were sintered at $1200^{\circ}C$ in an air atmosphere. The porous microstructure of the pre-forms was kept their original pattern after sintering with a synthesis of calcium phosphate. The obtained porous calcium phosphate, sintered at $1200^{\circ}C$ for 3 h at 17% concentration of phosphoric acid, showed uniform open pores of 150 ${\mu}m$ in size and $\beta$-TCP phase in the XRD patterns. Above 16% concentration, CaO phase, derived from the decomposition of $CaCO_3$, decreased gradually in the sintered samples, and the measured Ca/P ratios of the samples prepared from 16% and 18% concentration were 1.67 and 1.43, which are close to stoichiometric HA (1.66) and $\beta$-TCP (1.50).


Supported by : 목포대학교


  1. J. B. Park and R. S. Lakes, Biomaterials An Introduction; pp. 117-40, Plenum Press, New York, 1996.
  2. L. L. Hench, “Bioceramics,” J. Am. Ceram. Soc., 81 [7] 1705-28 (1998).
  3. S. R. Kim, Y. H. Kim, S. J. Jung, and D. H. Riu, “Synthesis and Characterization of Silicon Substituted Hydroxyapatite,” J. Kor. Ceram. Soc., 38 [12] 1132-36 (2001).
  4. H. J. Kim, S. C. Choi, J. W. Seok, and R. Telle, “Bone Cements in TTCP, DCPA, ${\beta}$-TCP and PHA System,” J. Kor. Ceram. Soc., 39 [1] 57-67 (2002).
  5. L. H. Lee and J. S. Ha, “Fabrication and Properties of Sioactive Porous Ceramics for Bone Substitution,” J. Kor. Ceram. Soc., 45 [10] 584-88 (2008).
  6. J. S. Choi, H. C. Park, and S. Y. Yoon, “Sr-containing Hydroxyapatite for Bone Replacement,” J. Kor. Ceram. Soc., 45 [10] 589-93 (2008).
  7. A. M. Gatti, D. Zaffe, and G. P. Poli, “Behavior of Tricalcium Phosphate and Hydroxyapatite Granules in Sheep Bone Defects,” Biomaterials, 11 513-60 (1990).
  8. N. O. Engin and A. C. Tas, “Preparation of Porous $Ca_{10} (PO_4)_6(OH)_2$ and ${\beta}–Ca_3(PO_4)_2$ Bioceramics,” J. Am. Ceram. Soc., 83 [7] 1581-84 (2000).
  9. H. S. Ryu, H. J. Youn, K. S. Hong, B. S. Chang, C. K. Lee, and S. S. Chung, “An Improvement in Sintering Property of b–Tricalcium Phosphate by Addition of Calcium Pyrophosphate,” Biomaterials, 23 99-104 (2002).
  10. A. Deptula, W. Lada, T. Olczak, A. Borello, C. Alvani, and A. Bartolomeo, “Preparation of Spherical Powders of Hydroxyapatite by Sol-Gel Process,” J. Non-Cryst. Solids., 147 537-41 (1992).
  11. M. Yoshimura, H. Suda, K. Okamoto, and K. Ioku, “Hydrothermal Synthesis of Biocompatible Whisker,” J. Mater. Sci., 29 [13] 3399-402 (1994).
  12. M. G. S. Murray, J. Wang, C. B. Ponton, and P. M. Marquis, “An Improvement in Processing of Hydroxyapatite Ceramics,” J. Mater. Sci., 30 [12] 3061-74 (1995).
  13. R. Famery, N. Richard, and P. Boch, “Preparation of α–and ${\beta}$–Tricalcium Phosphate Ceramics, With and Without Magnesium Addition,” Ceram. Int., 20 327-36 (1994).
  14. S. C. Liou and S. Y. Chen, “Transformation Mechanism of Different Chemically Precipitated Apatitic Precursors into ${\beta}$-Tricalcium Phosphate upon Calcinations,” Biomatcrials, 23 4541-47 (2002).
  15. S. Jinawath and P. Sujaridworakun, “Fabrication of Porous Calcium Phosphates,” Mater. Sci. and Eng., C22 41-6 (2002).
  16. D. M. Liu, “Fabrication and Characterization of Porous Hydroxyapatite Granules,” Biomatcrials, 17 1955-57 (1996).
  17. M. D. Kwon, S. H. Oh, and S. J. Lee, “Snthesis of ${\beta}$-Tricalcium Phosphate by Using an Eggshell,” J. Kor. Ceram. Soc., 39 [11] 1103-07 (2002).
  18. S. J. Lee, Y. S. Yoon, M. H. Lee, and N. S. Oh, “Highly Sinterable Beta-Tricalcium Phosphate Synthesized from Eggshell,” Mater. Lett., 61 1279-82 (2007).
  19. S. J. Lee, Y. S. Yoon, M. H. Lee, and N. S. Oh, “Highly Sinterable Beta-Tricalcium Phosphate Synthesized from Eggshell,” Mater. Lett., 61 1279-82 (2007).
  20. S. J. Lee, Y. S. Yoon, M. H. Lee, and N. S. Oh, “Nanosized Hydroxyapatite Powder Synthesized from Eggshell and Phosphoric Acid,” J. Nanoscience and Nanotechnology, 7 [11] 4061-64 (2007).
  21. S. J. Lee, Y. C. Lee, and Y. S. Yoon, “Characteristics of Calcium Phosphate Powders Synthesized from Cuttlefish Bone and Phosphoric Acid,” J. Ceram. Proc. Res., 8 [6] 427-30 (2007).
  22. M. Bohner, J. Lemaitre, A. P. Legrand, and P. Belgrand “Synthesis, X-ray Diffraction and Solid-State 31P Magic Angle Spinning NMR Study of ${\beta}$-Tricalcium Orthophosphate,” J. Mater. Sci: Mater. Med., 7 [7] 457-63 (1996).

Cited by

  1. Porous Biphasic Calcium Phosphate Scaffolds from Cuttlefish Bone vol.94, pp.8, 2011,
  2. Biomedical Materials for Regenerating Bone Tissue Utilizing Marine Invertebrate vol.48, pp.1, 2015,
  3. Hydroxyapatite from Cuttlefish Bone: Isolation, Characterizations, and Applications vol.23, pp.4, 2018,