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Development of Bioreactor for Regenerative Medicine and Effect of Mechanical Stimuli on Mesenchymal Stem Cells in Polyurethane Scaffolds

바이오리액터 개발과 기계적 자극에 의한 중간엽 줄기세포의 영향에 관한 연구

  • 주민진 (연세대학교 기계공학부) ;
  • 전흥재 (연세대학교 기계공학부) ;
  • 정형진 (연세대학교 기계공학부) ;
  • 이창근 (연세대학교 의과대학 의과학과) ;
  • 허동녕 (경희대학교 치의학대학원 구강생물학과) ;
  • 권일근 (경희대학교 치의학대학원 구강생물학과) ;
  • 문성환 (연세대학교 의과대학 의과학과)
  • Received : 2009.02.10
  • Accepted : 2010.03.23
  • Published : 2010.06.01

Abstract

It is well known that mesenchymal stem cell(MSCs) can be differentiated into fibroblasts, chondrocytes, and osteoblasts and that they develop into fibrous tissue, cartilage, or bone, as a result of mechanical stimulation. In this study, we developed a bioreactor system, which is composed of a reactor vessel that provides the required cell culture environment, an environment controlling chamber to control the media, a gas mixer, and a reactor motion control subsystem to apply mechanical stimuli to the cells. For the MSC culture, We used a poly-urethane (PU) scaffold, with a collagen coating to ensure improved cohesion ratio. Then, we transferred the cultivated MSCs in the PU scaffold, cultured the cells in the bioreactor system, and confirmed the proliferation, differentiation, and ossification processes, resulting from mechanical stimuli.

Keywords

Bioreactor;Mechanical Stimulation;Mesenchymal Stem Cell;Polyurethane Scaffold

Acknowledgement

Grant : 기계적자극에 의한 성체줄기세포의 분화 모델 및 생체역학적 BIOREACTOR를 이용한 근골격계 COMPUTER AIDED TISSUE ENGINEERING

Supported by : 한국과학재단

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