Characterization of the Biogenic Manganese Oxides Produced by Pseudomonas putida strain MnB1

  • Jiang, Shaofeng (Department of Civil Engineering, Kyung Hee University) ;
  • Kim, Do-Gun (Department of Civil Engineering, Kyung Hee University) ;
  • Kim, Jeong-Hyun (Department of Civil Engineering, Kyung Hee University) ;
  • Ko, Seok-Oh (Department of Civil Engineering, Kyung Hee University)
  • Received : 2010.09.10
  • Accepted : 2010.10.28
  • Published : 2010.12.30


Biogenic Mn oxides are expected to have great potential in the control of water pollution due to their high catalytic activity, although information on biological Mn oxidation is not currently sufficient. In this study, the growth of a Mn oxidizing microorganism, Pseudomonas putida MnB1, was examined, with the Mn oxides formed by this strain characterized. The growth of P. putida MnB1 was not significantly influenced by Mn(II), but showed a slightly decreased growth rate in the presence of Pb(II) and EE2, indicating their insignificant adsorption onto the cell surface. Mn oxides were formed by P. putida MnB1, but the liquid growth medium and resulting biogenic solids were poorly crystalline, nano-sized particles. Biogenic Mn oxidation by P. putida MnB1 followed Michaelis-Menten kinetics, with stoichiometric amounts of Mn oxides formed, which corresponded with the initial Mn(II) concentration. However, the formation of Mn oxides was inhibited at high initial Mn(II) concentration, suggesting mass transfer obstruction of Mn(II) due to the accumulation of Mn oxides on the extracellular layer. Mn oxidation by P. putida MnB1 was very sensitive to pH and temperature, showing sharp decreases in the Mn oxidation rates outside of the optimum ranges, i.e. pH 7.43-8.22 and around 20-$26^{\circ}C$.


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