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Variations of Immunoglobulins in Colostrum and Immune Milk as Affected by Antigen Releasing Devices

  • Zhaoa, Shengguo (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Zhanga, Chungang (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Wang, Jiaqi (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Liu, Guanglei (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Bu, Dengpan (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Cheng, Jinbo (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences) ;
  • Zhou, Lingyun (State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences)
  • Received : 2009.10.12
  • Accepted : 2009.12.10
  • Published : 2010.09.01

Abstract

This work was conducted to examine the variation of immunoglobulins (Igs) in serum, immune milk, normal milk and colostrum upon implantation of a new Antigen Releasing Device (ARD). The core of each ARD housed an immunostimulating complex (ISCOM) that was made of adjuvant Quil A and type XIII lipase from a Pseudomonas sp. Each ARD was coated with polylactic acid, known as polylactide, that controls antigen release. Twenty lactating Chinese Holstein cows were divided into 2 groups (n = 10): test group and control group. All cows in the test group were implanted with a single injection in the right iliac lymph node with 3 types of ARDs, which were designed to release the antigens at d 0, 14 and 28 post-implantation. Blood and milk samples were collected from both groups, and colostrum samples were also collected from other post-partum cows in the same farm. Concentrations of $IgG_1$, IgA and IgM in whey and serum were measured by sandwich ELISA. The results showed that the $IgG_1$, IgA and IgM concentrations in serum and whey from the test group were higher than from the control group. Among the three Igs measured, the $IgG_1$ concentration in serum was significantly higher at d 40 after ARD implantation, and the $IgG_1$ concentration in whey peaked at d 9, 17 and 30, which corresponded with release of the antigen. Based on Pearson's correlation between Ig concentration and production parameters, IgA concentration in normal milk was positively correlated with lactation period, which reflected IgA changes during the lactation period in immune milk. In colostrum, $IgG_1$, IgA and IgM decreased abruptly from d 0 to 3, and then decreased slightly. In conclusion, serum $IgG_1$ concentration can be affected by controlled release of the ARD, while whey IgA levels are primarily affected by lactation period. These results may be useful in future studies designed to regulate concentrations of Igs in immune milk.

Keywords

Immunoglobulin;Immune Milk;Colostrum;Antigen Releasing Device

References

  1. Anderson, C. L., C. Chaudhury, J. Kim, C. L. Bronson, M. A. Wani and S. Mohanty. 2006. Perspective-FcRn transports albumin: relevance to immunology and medicine. Trends Immunol. 27(7):343-348. https://doi.org/10.1016/j.it.2006.05.004
  2. Ashraf, H., D. Mahalanabis, A. K. Mitra, S. Tzipori and G. J. Fuchs. 2001. Hyperimmune bovine colostrum in the treatment of shigellosis in children: A double-blind, randomized, controlled trial. Acta Paediatr. 90(12):1373-1378. https://doi.org/10.1111/j.1651-2227.2001.tb01598.x
  3. Besser, T. E. and C. C. Gay. 1994. The importance of colostrum to the health of the neonatal calf. Vet. Clin. North Am. Food Anim. Pract. 10(1):107-117.
  4. Bush, L. J. and T. E. Staley. 1980. Absorption of colostral immunoglobulins in newborn calves. J. Dairy Sci. 63(4):672-680. https://doi.org/10.3168/jds.S0022-0302(80)82989-4
  5. Butler, J. E. 1983. Bovine immunoglobulins: an augmented review. Vet. Immunol. Immunopathol. 4(1-2):143-152.
  6. Butler, J. E. 1998. Immunoglobulin diversity, B cell and antibody repertoire development in large farm animals. Rev. Sci. Technol. 17(1):43-70.
  7. Caffin, J. P., B. Poutrel and P. Rainard. 1983. Physiological and pathological factors influencing bovine immunoglobulin $G_1$ concentration in milk. J. Dairy Sci. 66(10):2161-2166. https://doi.org/10.3168/jds.S0022-0302(83)82063-3
  8. Cheng, J. B., J. Q. Wang, D. P. Bu, G. L. Liu, C. G. Zhang, H. Y. Wei, L. Y. Zhou and J. Z. Wang. 2008. Factors affecting the lactoferrin concentration in bovine milk. J. Dairy Sci. 91:970-976. https://doi.org/10.3168/jds.2007-0689
  9. Claerebout, E. and J. Vercruysse. 2000. The immune response and the evaluation of acquired immunity against gastrointestinal nematodes in cattle: a review. Parasitology 120:25-42. https://doi.org/10.1017/S0031182099005776
  10. Grosvenor, C. E., M. F. Picciano and C. R. Baumrucker. 1993. Hormones and growth factors in milk. Endocrinol. Rev. 14(6):710-728. https://doi.org/10.1210/edrv-14-6-710
  11. Kolb, F. A. 2002. Engeneering immunity in the mammary gland. J. Mammary. Gland Biol. 7(2):123-134. https://doi.org/10.1023/A:1020395701887
  12. Korhonen, H., P. Marnila and H. S. Gill. 2000. Bovine milk antibodies for health. Br. J. Nutr. 84(Suppl 1):S135-S146.
  13. Korhonen, H., P. Marnila and H. S. Gill. 2000. Milk immunoglobulins and complement factors. Br. J. Nutr. 84(Suppl 1):S75-S80.
  14. Levieux, D. and A. Ollier. 1999. Bovine immunoglobulin G, beta-lactoglobulin, alpha-lactalbumin and serum albumin in colostrum and milk during the early post partum period. J. Dairy. Res. 66(3):421-430. https://doi.org/10.1017/S0022029999003581
  15. Lilius, E. M. and P. Marnila. 2001. The role of colostral antibodies in prevention of microbial infections. Curr. Opin. Infect. Dis. 14(3):295-300. https://doi.org/10.1097/00001432-200106000-00008
  16. Liu, G. L., J. Q. Wang, D. P. Bu, J. B. Cheng, C. G. Zhang, H. Y. Wei, L. Y. Zhou, Z. F. Zhou, H. Hu and X. L. Dong. 2009. Factors affecting the transfer of immunoglobulin G1 into the milk of Holstein cows. Vet. J. 182:79-85. https://doi.org/10.1016/j.tvjl.2008.05.019
  17. Liu, G. L., J. Q. Wang, D. P. Bu, J. B. Cheng, C. G. Zhang, H. Y. Wei, L. Y. Zhou, K. L. Liu and X. L. Dong. 2009. Specific immune milk production of cows implanted with antigen-release devices. J. Dairy Sci. 92(1):100-108. https://doi.org/10.3168/jds.2008-1114
  18. Lo, W. C. and R. E. Kleiman. 1996. Infant formula, past and future: opportunities for improvement. Am. J. Clin. Nutr. 63(4): 646-654.
  19. Marnila, P., S. Rokka, L. Rehberg-Laiho, P. Karkkainen, T. U. Kosunen, H. Rautelin, M. L. Hanninen, E. L. Syvaoja and H. Korhonen. 2003. Prevention and suppression of Helicobacter felis infection in mice using colostral preparation with specific antibodies. Helicobacter 8(3):192-201. https://doi.org/10.1046/j.1523-5378.2003.00144.x
  20. McFadden, T. B., T. E. Besser and G. M. Barrington. 1997. Regulation of immunoglobulin transfer into mammary secretions of ruminants. In: Milk composition, production and biotechnology in Agricultural research (Ed. D. J. W. Welch, S. R. Burns, S. R. Davis, A. I. Popay and C. G. Prosser). CAB International, Wallingford, pp. 133-152.
  21. Mehra, R., P. Marnila and H. Korhonen. 2006. Milk immunoglobulins for health promotion. Int. Dairy J. 16:1262-1271 https://doi.org/10.1016/j.idairyj.2006.06.003
  22. Mero, A., H. Miikkulainen, J. Riski, R. Pakkanen, J. Aalto and T. Takala. 1997. Effects of bovine colostrum supplementation on serum IGF-1, IgG, hormone, and saliva IgA during training. J. Appl. Physiol. 83(4):1144-1151.
  23. Pearse, M. J. and D. Drane. 2004. ISCOMATRIXTM adjuvant: a potent inducer of humoral and cellular immune responses. Vaccine 19:2391-2395.
  24. Quigley, J. D., K. R. Martin, H. H. Dowlen, L. B. Wallis and K. Lamar. 1994. Immunoglobulin concentration, specific gravity, and nitrogen fractions of colostrum from Jersey cattle. J. Dairy Sci. 77(1):264-269. https://doi.org/10.3168/jds.S0022-0302(94)76950-2
  25. Sanchez, J., F. Markhamb, I. Dohoo, J. Sheppard, G. Keefe and K. Leslie. 2004. Milk antibodies against Ostertagia ostertagi: relationship with milk IgG and production parameters in lactating dairy cattle. Vet. Parasitol. 120(4):319-330. https://doi.org/10.1016/j.vetpar.2004.01.010
  26. Tawfeek, H. I., N. H. Najim and S. Al-MAshikhi. 2003. Efficacy of an infant formula containing anti-Escherichia coli colostral antibodies from hyperimmunized cows in preventing diarrhea in infants and children: A field trial. Int. J. Infect. Dis. 7(2):120-128. https://doi.org/10.1016/S1201-9712(03)90007-5
  27. Vaccaro, C., J. Zhou, R. J. Ober and E. S. Ward. 2005. Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels. Nat. Biotechnol. 23(10):1283-1288. https://doi.org/10.1038/nbt1143