Comparison of Rectal and Infrared Thermometry for Obtaining Body Temperature of Gnotobiotic Piglets in Conventional Portable Germ Free Facility

Chung, Tae-Ho;Jung, Woo-Sung;Nam, Eui-Hwa;Kim, Ji-Hyun;Park, Seol-Hee;Hwang, Cheol-Yong

  • Received : 2009.09.29
  • Accepted : 2010.01.25
  • Published : 2010.10.01


Gnotobiotic piglets (n = 10) were hand-reared in conventional germ-free facilities. Piglet body temperatures were measured with rectal and non-contact infrared thermometry (NIFT) on the lower eyelid, auricular center and margin, parietal regions, axilla, central abdomen and dorsum, and the perianal region. Body temperature measurements at central abdomen, cranial dorsum, and perianal regions had NIFT values which had a significant linear relationship (p<0.0001) with rectal thermometry. The predicted equations of between-subject formulas were calculated as follows: rectal temperature, 28.07489+0.30372${\times}$central abdominal surface temperature; rectal temperature, 34.02799+0.15197${\times}$central dorsum surface temperature; and rectal temperature, 33.87937+0.15676${\times}$perianal temperature. These results suggested that NIFT could serve as a valid alternative to rectal thermometry in a portable germ-free facility without disturbing experimental animals. The development of a NIFT body temperature evaluation that does not require animal restraint is clinically advantageous, particularly in gnotobiotic piglets, and would be significantly less stressful for experimental procedures in germ-free facilities.


Gnotobiotic Piglet;Rectal Temperature;Infrared Thermometry;Portable Germ Free Facility


  1. Bayne, K. 1996. Revised guide for the care and use of laboratory animals available. American Physiological Society. Physiologist 39:199.
  2. Chapman, L., T. Folks, D. Salomon, A. Patterson, T. Eggerman and P. Noguchi. 1995. Xenotransplantation and xenogeneic infections. N. Engl. J. Med. 333:1498-1501.
  3. Chatterjee, S. and A. Hadi. 2006. Regression analysis by example. John Wiley and Sons.
  4. Chen, P. and C. White. 2006. Comparison of rectal, microchip transponder, and infrared thermometry techniques for obtaining body temperature in the laboratory rabbit (Oryctolagus cuniculus). J. Am. Assoc. Lab. Anim. Sci. 45:57-63.
  5. Coates, M. 1975. Gnotobiotic animals in research: their uses and limitations. Lab. Anim. 9:275.
  6. Dollberg, S., Y. Xi and M. Donnelly. 1993. A noninvasive transcutaneous alternative to rectal thermometry for continuous measurement of core temperature in the piglet. Pediatr. Res. 34:512.
  7. Goodwin, S. 1998. Comparison of body temperatures of goats, horses, and sheep measured with a tympanic infrared thermometer, an implantable microchip transponder, and a rectal thermometer. Contemp. Top. Lab. Anim. Sci. 37:51.
  8. Greenes, D. and G. Fleisher. 2001. Accuracy of a noninvasive temporal artery thermometer for use in infants. Arch. Pediatr. Adolesc. Med. 155:376.
  9. Loughmiller, J., M. Spire, S. Dritz, B. Fenwick, M. Hosni and S.Hogge. 2001. Relationship between mean body surface temperature measured by use of infrared thermography and ambient temperature in clinically normal pigs and pigs inoculated with Actinobacillus pleuropneumoniae. Am. J. Vet. Res. 62:676-681.
  10. Meyer, R., E. Bohl and E. Kohler. 1964. Procurement and Maintenance of Germ-Free Swine for Microbiological Investigations 1. Appl. Environ. Microbiol. 12:295-300.
  11. Newsom, D., G. Bolgos, L. Colby and J. Nemzek. 2004.Comparison of body surface temperature measurement and conventional methods for measuring temperature in the mouse. Contemp. Top. Lab. Anim. Sci. 43:13.
  12. Ng, D., C. Chan, R. Lee and L. Leung. 2005. Non-contact infrared thermometry temperature measurement for screening fever in children. Annals of Tropical Paediatrics: Int. Child Health 25:267-275.
  13. Saegusa, Y. and H. Tabata. 2003. Usefulness of infrared thermometry in determining body temperature in mice. J. Vet. Med. Sci. 12:1365-1367.
  14. Thoresen, M., M. Simmonds, S. Satas, J. Tooley and I. Silver. 2001. Effective selective head cooling during posthypoxic hypothermia in newborn piglets. Pediat. Res. 49:594.
  15. Tucker, A., C. Belcher, B. Moloo, J. Bell, T. Mazzulli, A. Humar,A. Hughes, P. McArdle and A. Talbot. 2002. The production of transgenic pigs for potential use in clinical xenotransplantation: microbiological evaluation. Xenotransplantation 9:191-202.
  16. Yuan, L., L. Ward, B. Rosen, T. To and L. Saif. 1996. Systematic and intestinal antibody-secreting cell responses and correlates of protective immunity to human rotavirus in a gnotobiotic pig model of disease. J. Virol. 70:3075-3083.

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