Difference of Gene Expression between Hypertrophic Scar Keratinocytes and Normal Keratinocytes

비후성 반흔 각질세포와 정상 각질세포의 유전자 비교분석

  • Choi, Sung-Won (Department of Plastic and Reconstructive Surgery, Fatima Hospital) ;
  • Chung, Ho-Yun (Department of Plastic and Reconstructive Surgery, School of Medicine, Kyngpook National University) ;
  • Lim, Young-Kook (Department of Plastic and Reconstructive Surgery, Fatima Hospital) ;
  • Kim, Hoon-Nam (Department of Plastic and Reconstructive Surgery, Fatima Hospital) ;
  • Oh, Ji-Won (Department of Immunology, School of Medicine, Kyngpook National University) ;
  • Kim, Moon-Kyu (Department of Immunology, School of Medicine, Kyngpook National University) ;
  • Jeon, Sae-Hwa (Tego Science Inc.) ;
  • Hong, Yong-Taek (Department of Plastic and Reconstructive Surgery, Fatima Hospital)
  • 최성원 (대구파티마병원 성형외과) ;
  • 정호윤 (경북대학교 의학전문대학원 성형외과학교실) ;
  • 임영국 (대구파티마병원 성형외과) ;
  • 김훈남 (대구파티마병원 성형외과) ;
  • 오지원 (경북대학교 의학전문대학원 면역학교실) ;
  • 김문규 (경북대학교 의학전문대학원 면역학교실) ;
  • 전세화 (테고 사이언스(주)) ;
  • 홍용택 (대구파티마병원 성형외과)
  • Received : 2010.04.08
  • Accepted : 2010.06.09
  • Published : 2010.07.10

Abstract

Purpose: There is no clear evidence of the original cause of hypertrophic scar, and the effective method of treatment is not yet established. Recently the steps of searching in gene and molecular level are proceeding. we are trying to recognize the difference between keratinocytes of hypertrophic scar and normal skin. Then we do support the comprehension of the scar formation mechanism and scar management. Methods: Total RNAs were extracted from cultured keratinocytes from 4 hypertrophic scars and normal skins. The cDNA chips were prepared. A total of 3063 cDNAs from human cDNA library were arrayed. And the scanning data were analyzed. Results: On microarray, heat shock protein, pyruvate kinase, tumor rejection antigen were more than 2 fold intensity genes. Among them, heat shock 70 kd protein showed the strongest intensity difference. Conclusion: In this study, it can be concluded that heat shock proteins play an important role in the process of wound healing and scar formation. This study provides basic biologic information for scar research. The new way of the prevention and treatment of scar formation would be introduced with further investigations.

Acknowledgement

Grant : 지역거점연구 육성사업

Supported by : 노화극복. 웰빙을 위한 융합의료기술개발사업단

References

  1. Wolfram D, Tzankov A, Pulzl P, Piza-Katzer H: Hypertrophic scars and keloids-a review of their pathophysiology, risk factor and therapeutic management. Dermatol surg 35: 171, 2009 https://doi.org/10.1111/j.1524-4725.2008.34406.x
  2. Choi SR, yoon MH, Dong ES, Yoon ES: A combined therapy of steroid injection, silicone gel sheeting, and laser for hypertrophic scar and keloid. J Korean Soc Plast Reconstr Surg 33: 700, 2006
  3. YJ Kim: Modulatory effect of TGF-beta 2 to proliferative kinetics of fibroblast in keloid and hypertrophic scar. J Korean Soc Plast Reconstr Surg 30: 194, 2003
  4. Colwell As, Phan TT, Kong W, Longaker MT, Lorenz PH: Hypertrophic scar fibroblasts have increased connective tissue growth factor expression after transforming growth factor-bata stimulation. Plast Reconstr Surg 116: 1387, 2005 https://doi.org/10.1097/01.prs.0000182343.99694.28
  5. Shukla A, Dubery MP, Srivastava R, Srivastava BS: Differential expression of proteins during healing of cutaneous wounds in experimental normal and chronic models. Biochem Biophys Res Commun 244: 434, 1998 https://doi.org/10.1006/bbrc.1998.8286
  6. Machesney M Tidman N, Waseem A, Kirby L, Leigh l: Activated keratinocytes in the epidermis of hypertrophic scars. Am J Pathol 152: 1133, 1998
  7. Chung EJ, Sung YK, Farooq M, Kim Y, Im S, Tak WY, Hwang YJ, Kim YI, Han HS, Kim JC, Kim MK: Gene expression profile analysis in human hepatocellular carcinoma by cDNA microarray. Mol Cells 14: 382, 2002
  8. Shin SY, Chang DM, Kim YJ, Lee BK, Wee SS, Ahn ST: The Effect of tumor necrosis factor-alpa on typeI procollagen and collagenase gene expression in hypertrophic scar and keloid fibroblast. J Korean Soc Plast Reconstr Surg 28: 145, 2001
  9. Park DM, Sohn DG, Han KH, Lee SY, Chae YM, Chang YC, Park KK: The effect of the transcriptional regulation of Sp1 for TGF-beta1 and CTGF expression in scar formation. J Korean Soc Plast Reconstr Surg 33: 39, 2006
  10. Lim CP, Phan TT, Lim IJ, Cao X: Cytokine profiling and stat3 phosphorylation in epithelial-mesenchymal interac-tions between keloid keratinocytes and fibroblasts. J Invest Dermatol 129: 851, 2009 https://doi.org/10.1038/jid.2008.337
  11. Tandara AA, Kloeters O, Mogford JE, Mustoe TA: Hydrated keratinocytes reduce collagen synthesis by fibroblasts via paracrine mechanisms. Wound Repair Regen 15: 497, 2007 https://doi.org/10.1111/j.1524-475X.2007.00257.x
  12. Barrow RE, Dasu MR: Oxidative and heat stress gene change in hypertrophic scar fibroblasts stimulated with interleukin-1beta. J Surg Res 126: 59, 2005 https://doi.org/10.1016/j.jss.2005.01.011
  13. Oberringer M, Baum HP, Jung V, Welter C, Frank J, Kuhlmann M, Mutschler W, Hanselmann RG: Differential expression of heat shock protein 70 in well healing and chronic human wound tissue. Biochem Biophys Res Commun 214: 1009, 1995 https://doi.org/10.1006/bbrc.1995.2386
  14. Lu F, Gao J, Ogawa R, Hyakusoku H, Ou C: Fas-mediated apoptotic signal transduction in keloid and hypertrophic scar. Plast Reconstr Surg 119: 1714, 2007 https://doi.org/10.1097/01.prs.0000258851.47193.06
  15. Arya R, Mallik M, Lakhotia SC: Heat shock genes-integrating cell survival and death. J Biosci 32: 595, 2007 https://doi.org/10.1007/s12038-007-0059-3