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Prediction and Annotation of ABC Transporter Genes from Magnaporthe oryzae Genome Sequence

벼도열병균 게놈서열로부터 ABC transporter 유전자군의 예측 및 특성 분석

  • Kim, Yong-Nam (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Jin-Soo (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Su-Young (Department of Biomaterial Control, Dong-Eui University) ;
  • Kim, Jeong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Lee, Jong-Hwan (Department of Biomaterial Control, Dong-Eui University) ;
  • Choi, Woo-Bong (Department of Biomaterial Control, Dong-Eui University)
  • 김용남 (동의대학교 바이오물질제어학과) ;
  • 김진수 (동의대학교 바이오물질제어학과) ;
  • 김수영 (동의대학교 바이오물질제어학과) ;
  • 김정환 (동의대학교 바이오물질제어학과) ;
  • 이종환 (동의대학교 바이오물질제어학과) ;
  • 최우봉 (동의대학교 바이오물질제어학과)
  • Received : 2009.12.02
  • Accepted : 2009.12.10
  • Published : 2010.02.28

Abstract

Magnaporthe oryzae is destructive plant-pathogenic fungus and causes rice blast. The pathogen uses several mechanisms to circumvent the inhibitory actions of fungicides. ATP-binding cassette (ABC) transporters are known to provide protection against toxic compounds in the environment. PC facilitated bioinformatic analysis, particularly with respect to accessing and extracting database information and domain identification. We predicted ABC transporter genes from the M. oryzae genome sequence with computation and bioinformatics tools. A total of thirty three genes were predicted to encode ABC transporters. Three of thirty three putative genes corresponded to three known ABC transporter genes (ABC1, ABC2 and ABC3). Copy numbers of the ABC transporter genes were proven by Southern blot analysis, which revealed that twenty genes tested exist as a single copy. We amplified the DNA complementary to RNA corresponding to eleven of these by reverse transcriptase polymerase chain reaction.

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