Optimum Conditions for the Culture of Hericium erinaceum in a Jar Fermenter with the Addition of Ginseng Extract in the Liquid Medium

수삼추출물을 이용한 노루궁뎅이버섯 균사체의 jar fermenter에서의 발효조건 최적화

  • Park, Chang-Kyu (Division of Food and Biotechnology, Chungju National University) ;
  • Tu, Qi (Division of Food and Biotechnology, Chungju National University) ;
  • Cho, Ju-Hyun (Hurum Central Research Institute) ;
  • Yu, Kwang-Won (Division of Food and Biotechnology, Chungju National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University) ;
  • Lee, Hyeon-Yong (Department of Biomaterials Engineering, Kangwon National University) ;
  • Jeong, Jae-Hyun (Division of Food and Biotechnology, Chungju National University)
  • 박창규 (충주대학교 식품생명공학부) ;
  • 도기 (충주대학교 식품생명공학부) ;
  • 조주현 ((주)휴럼 중앙연구소) ;
  • 유광원 (충주대학교 식품생명공학부) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 이현용 (강원대학교 생물소재공학) ;
  • 정재현 (충주대학교 식품생명공학부)
  • Received : 2009.10.06
  • Accepted : 2009.12.05
  • Published : 2010.02.28

Abstract

To obtain functional materials from a submerged culture of Hericium erinaceum, a suitable basal medium for flask culture was screened and the optimal culture conditions in a jar fermenter were investigated with the addition of ginseng extracts (GE) to the basal liquid medium. Of all tested basal liquid media, the mushroom complete medium (MCM) supplemented with 0.5% of GE produced the highest mycelial dry weight (MDW) of 5.91 g/L in the flask, which reached a plateau at $25^{\circ}C$, pH 5.5 after 10 days. The submerged culture conditions for the mass production of mycelia in a 50 L jar fermenter were also optimal at $25^{\circ}C$, pH 5.5, 120 rpm agitation speed and 0.4 vvm aeration rate. Under these conditions, the maximum MDW was produced, which reached a value of 4.28 g/L within 5 days. When we investigated the effects of the amount of GE in the MCM on the production of MDW in the jar fermenter, the addition of 5% GE (HE-GE-5) under the optimal culture conditions produced the maximum MDW (4.93 g/L). In addition, the crude polysaccharide of HE-GE-5 contained mainly neutral sugars (63.2%) with considerable amounts of uronic acid (19.3%) and a small amount of proteins (8.8%) and it had potent immunostimulation properties.

Keywords

Hericium erinaceum;ginseng extract;submerged culture;mycelium dry weight;jar fermenter

Acknowledgement

Supported by : 농촌진흥청

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