DOI QR코드

DOI QR Code

Comparative Analysis of the Antioxidant Activities of Green Peppers Cultivated under Conventional and Environmental-Friendly Farming conditions

Choi, Jang-Yeol;Choi, Yeo-Jin;Lee, Seong-Gene

  • 투고 : 2010.02.11
  • 심사 : 2010.03.09
  • 발행 : 2010.03.31

초록

Environmental-friendly agriculture (EFA) is defined as the cultivation of crops with reduced amounts or without chemical-synthetic pesticides. Recently, the use of chemical pesticides has decreased significantly; therefore, we cultivated peppers following EFA- and conventional methods and compared their antioxidant activities. To accomplish this, the environmental-friendly cultivated peppers (EFPE) and conventionally cultivated peppers (CCPE) were extracted with 70% methanol and the effects of the extracts on the cell viability, intracellular ROS generation, lipid peroxidation and catalase activity of HepG2 cells were evaluated. EFPE showed a stronger protective effect against oxidative stress induced-cell death than that of CCPE. EFPE also reduced intracellular ROS generation (42.7% to 26.4%) following treatment with hydrogen peroxide more effectively than that of CCPE (24.2% to 6.3%). Furthermore, EFPE and CCPE showed protective effects against lipid peroxidation and induced catalase activity, although these effects were not statistically significant. Taken together, these results suggest that EFPE showed stronger antioxidant activities than CCPE, and thus represent evidence that EFA with biocontrol materials may improve the functional properties of crops and/or secondary metabolites with antioxidant activities when compared with conventional agricultural practices.

키워드

Antioxidant;Catalase;Environmental-Friendly Agriculture;Intracellular ROS;Lipid Peroxidation;Pepper

참고문헌

  1. Anandakumar, P., Kamaraj, S., Jagan, S., Ramakrishnan, G., Vinodhkumar, R., Devaki, T., 2008. Capsaicin modulates pulmonary antioxidant defense system during benzo(a)pyrene-induced lung cancer in Swiss albino mice. Phytother. Res. 22, 529-533. https://doi.org/10.1002/ptr.2393
  2. Asami, D.K., Hong, Y.J., Barrett, D.M., Mitchell, A.E., 2003. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marionberry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices, J. Agric. Food Chem. 51, 1237-1241. https://doi.org/10.1021/jf020635c
  3. Barclay, R.C., Baskin, K.A., Dakin, K.A., Locke, S.J., Vinqvist, M.R., 1990. The antioxidant activities of phenolic antioxidants in free radical peroxidation of phospholipid membranes, Can. J. Chem. 68, 2258-2269. https://doi.org/10.1139/v90-348
  4. Block, G., 1992. The data support a role for antioxi-dants in reducing cancer risk, Nur. Rev. 50, 207-213.
  5. Brandt, K., Molgaard, J.P., 2001. Organic agriculture: Does it enhance or reduce the nutritional value of plant food?, J. Sci. Food Agric. 81, 924-931. https://doi.org/10.1002/jsfa.903
  6. Curtin, J.F., Donovan, M., Cotter, T.G., 2002. Regulation and measurement of oxidative stress in apoptosis, J. Immunol. Methods 265, 49-72. https://doi.org/10.1016/S0022-1759(02)00070-4
  7. Daood, H.G., Vinkler, M., Markus, F., Hebshi, E.A., Biacs, P.A., 1996. Antioxidant vitamin content of spice red pepper paprika as affected by techno-logical and varietal factors, Food Chem. 55, 365-372. https://doi.org/10.1016/0308-8146(95)00136-0
  8. Hussain, S.P., Hofseth, L., Harris, C.C., 2003. Radical causes of cancer, Nature Rev. Cancer. 3, 276-285. https://doi.org/10.1038/nrc1046
  9. Kahl, R., 1984. Synthetic antioxidants: biochemical actions and interference with radiation, toxic com-pounds, chemical mutagens and chemical carcinogens, Toxicology 33, 185-228. https://doi.org/10.1016/0300-483X(84)90038-6
  10. Kozukue, N., Han, J.S., Kozukue, E., Lee, S.J., Kim, J.A., Lee, K.R., Levin, C.E., Friedman, M., 2005. Analysis of eight capsaicinoids in peppers and pepper-containing foods by high-performance liquid chromatography and liquid chromatography-mass spectrometry, J. Agric. Food Chem. 53, 9172-9181. https://doi.org/10.1021/jf050469j
  11. Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoshi, A.E., Hilpert, K.F., Griel, A.E., Etherton, T.D., 2002. Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer, Am. J. Med. 113, 71S-88S. https://doi.org/10.1016/S0002-9343(01)00995-0
  12. Krinsky, N.I., 2001. Carotinoids as antioxidants, Nutrition 17, 815-817. https://doi.org/10.1016/S0899-9007(01)00651-7
  13. LeBel, C.P., Ishiropoulos, H., Bondy, S.C., 1992. Evaluation of the probe 2’,7’-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress, Chem. Res. Toxicol. 5, 227-231. https://doi.org/10.1021/tx00026a012
  14. Leonard, S.S., Harris, G.K., Shi, X., 2004. Metal-induced oxidative stress and signal transduction, Free Radic. Biol. Med. 37, 1921-1942. https://doi.org/10.1016/j.freeradbiomed.2004.09.010
  15. Luqman, S., Rizvi, S.I., 2006. Protection of lipid peroxidation and carbonyl formation in proteins by capsaicin in human erythrocytes subjected to oxidative stress. Phytother. Res. 20, 303-306. https://doi.org/10.1002/ptr.1861
  16. Ly, D., Kang, K., Choi, J.Y., Ishihara, A., Back, K., Lee, S., 2008. HPLC analysis of serotonin, tryp-tamine, tyramine, and the hydroxycinnamic acid amides of serotonin and tyramine in food vegetables, J. Med. Food 11, 385-389. https://doi.org/10.1089/jmf.2007.514
  17. Ly, D., Back, K., Kim, I.S., Kim, K.Y., Lee, S., 2008. Evaluation of Antioxidant Activities and Levels of Biogenic Monoamines of Plants Grown in Soil Amended with Chitin-Enriched Compost Harboring Chitinase-Producing Bacteria CECB, Acta Agric. Scand. Sect. B-Soil Plant Sci. 58, 379-384.
  18. Materska, M., Perucka, I., 2005. Antioxidant activity of the main phenolic compounds isolated from hot pepper fruit Capsicum annuum L., J. Agric. Food Chem. 53, 1750-1756. https://doi.org/10.1021/jf035331k
  19. Matsufuji, H., Nakamura, H., Chino, M., Takeda, M., 1998. Antioxidant activity of capsantin and the fatty acid esters in paprika Capsicum annuum, J. Agric. Food Chem. 46, 3468-3472. https://doi.org/10.1021/jf980200i
  20. Niki, E., Yoshida, Y., Saito, Y., Noguchi, N., 2005. Lipid peroxidation: mechanisms, inhibition, and biological effects, Biochem. Biophys. Res. Commun. 338, 668-676. https://doi.org/10.1016/j.bbrc.2005.08.072
  21. Niki, E., 2009. Lipid peroxidation: physiological levels and dual biological effects, Free Radic. Bio. Med. 47, 469-484. https://doi.org/10.1016/j.freeradbiomed.2009.05.032
  22. Olsson, M.E., Andersson, C.S., Oredsson, S., Berglund, R.H., Gustavsson, K.E., 2006. Antioxidant levels and inhibition of cancer cell proliferation in vitro by extracts from organically and conventionally culti-vated strawberries, J. Agric. Food Chem. 54, 1248-1255. https://doi.org/10.1021/jf0524776
  23. Perez-Lopez, A.J., Lopez-Nicolas, J.M., Nunez-Delicado, E., Del Amor, F.M., Carbonell-Barrachina, A.A., 2007. Effects of agricultural practices on color, carotenoids composition, and minerals contents of sweet peppers, cv. Almuden, J. Agric. Food Chem. 55, 8158-8164. https://doi.org/10.1021/jf071534n
  24. Prior, R.L., 2003. Fruits and vegetables in the pre-vention of cellular oxidative damage, Am. J. Clin. Nut. 78, 570S-578S. https://doi.org/10.1093/ajcn/78.3.570S
  25. Qiao, S., Li, W., Tsubouchi, R., Murakami, K., Yoshino, M., 2004. Role of vanilloid receptors in the cap-saicin- mediated induction of iNOS in PC12 cells, Neurochem. Res. 29, 687-693. https://doi.org/10.1023/B:NERE.0000018839.59457.5c
  26. Sato, T., Kawamoto, A., Tamura, A., Tatsumi, Y., Fuji, T., 1992. Mechanism of antioxidant action of pue-raris glycoside PG-1 an isoflavonoid and mangi-ferin a xanthonoid, Chem. Pharm. Bull. 40, 721-724. https://doi.org/10.1248/cpb.40.721
  27. Simon, D.A., Hidalgo-Diaz, L., Kalisz, H., Mauchline, T.H., 2003. Hirsch PR and Kerry BR, Development of a new management strategy for the control of rootknot nematodes Meloidogyne spp. in organic vegetable production, Pest Manag. Sci. 59, 183-189. https://doi.org/10.1002/ps.603
  28. Squier, T.C., 2001. Oxidative stress and protein aggregation during biological aging. Exp. Gerontol. 36, 1539-155. https://doi.org/10.1016/S0531-5565(01)00139-5
  29. Sukrasno, N., Yeoman, M.M., 1993. Phenylpropanoid metabolism during growth and development of Capsicum frutescens fruit. Phytochemistry 32, 839-844. https://doi.org/10.1016/0031-9422(93)85217-F
  30. Surh, Y.J., 2003. Cancer chemoprevention with dietary phytochemicals, Nat. Rev. Cancer 3, 768-780. https://doi.org/10.1038/nrc1189
  31. Valko, M., Leibfritz, D., Moncol, J., Cronin, M., Mazur, M., Telser, J., 2007. Free radicals and antioxi-dants in normal physiological functions and human disease, Int. J. Biochem. Cell Biol. 39, 44-84. https://doi.org/10.1016/j.biocel.2006.07.001
  32. Wachtel, R.E., 1999. Capsaicin, Region Anesth. Pain Med. 24, 361-363. https://doi.org/10.1097/00115550-199924040-00015
  33. Wang, S.Y., Chen, C.T., Sciarappa, W., Wang, C.Y., Camp, M.J., 2008. Fruit quality, antioxidant capacity, and flavonoid content of organically and conven-tionally grown blueberries, J. Agric. Food Chem. 56, 5788-5794. https://doi.org/10.1021/jf703775r