Study on the immobilization of plant glutathione S-transferase for development of herbicide detection kit

제초제 검출 키트 개발을 위한 식물 해독효소 고정화 연구

  • Cho, Hyun-Young (Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Lee, Jin-Joo (Department of Chemistry, College of Natural Sciences, Chung-Ang University) ;
  • Kong, Kwang-Hoon (Department of Chemistry, College of Natural Sciences, Chung-Ang University)
  • 조현영 (중앙대학교 자연과학대학 화학과) ;
  • 이진주 (중앙대학교 자연과학대학 화학과) ;
  • 공광훈 (중앙대학교 자연과학대학 화학과)
  • Received : 2010.02.10
  • Accepted : 2010.02.16
  • Published : 2010.04.25


Glutathione S-transferase is known to play a crucial role in detoxification in many cases. To develop a herbicide detection biosensor, we in this study attempted to immobilize glutathione S-transferase enzyme on solid supports, polystyrene and agarose, and Na-alginate. These matrixes were attractive materials for the construction of biosensors and might also have utility for the production of immobilized enzyme bioreactors. We also compared the activities of glutathione-S-transferase immobilized OsGSTF3 and free OsGSTF3. The specific activity of the free enzyme in solution was 3.3 higher than the immobilized enzyme. These results suggest that 50% of the enzyme was bound with the catalytic site in polystyrene-alkylamine bead and immobilized enzymes showed 80% remaining activity until 3 times reuse.


Supported by : 중앙대학교


  1. D. Weinrich, P. Jonkheijm, C. Niemeyer and H. Waldmann, Angew. Chem. Int. Edit., 48, 7744-7751(2009).
  2. D. Brady and J. Jordaan, Biotechnol. Lett., 31, 1639-1650(2009).
  3. U. Hanefeld, L. Gardossi and E. Magner, Chem. Soc. Rev., 38, 453-468(2009).
  4. Y. Kumada, Y. Tokunaga, H. Imanaka, K. Imamura, T. Sakiyama, S. Katoh and K. Nakanishi, Biotechnol. Prog., 22, 401(2006).
  5. N. Allocati, L. Federici, M. Masulli and C. D. Ilio, FEBS J., 276, 58(2009).
  6. D. P. Dixon, A. Lapthorn and R. Edwards. Genome Biol., 3, REVIEWS3004. (2002)
  7. R. N. Armstrong, Chem. Res. Toxicol., 4, 131(1987).
  8. D. Sheehan, G. Meade, V. M. Foley and C. A. Dowd, Biochem. J., 360, 1(2001).
  9. W. R. Pearson, Methods Enzymol., 401, 186(2005).
  10. R. Edwards and D. P. Dixon, Methods Enzymol., 401, 169(2005).
  11. H.-Y. Cho and K.-H. Kong, BioFactors, 30, 281(2007).
  12. M. Jain, C. Ghanashyam and A. Bhattacharjee, BMC Genomics, 11, 73(2010).
  13. H.-Y. Cho and K.-H. Kong, Pestic. Biochem. Physiol., 83, 29(2005).
  14. M. M. Bradford, Anal. Biochem., 72, 248(1976).
  15. U. K. Laemmli, Nature., 227, 680(1970).
  16. L. A. Delouise and B. L. Miller, Anal. Chem., 77, 1950 (2005).