Determination of Optimum Hydrolysis Conditions for Flavonoid Analysis in Plant Leaves

식물체 플라보노이드 성분 분석을 위한 적정 가수분해 조건

  • Park, Jin-Soon (Department of Food Science & Technology, Kyungpook National University) ;
  • Hwang, In-Wook (Department of Food Science & Technology, Kyungpook National University) ;
  • Zheng, Hu-Zhe (Department of Food Science & Technology, Kyungpook National University) ;
  • Kim, Suk-Kyung (Food and Bio-industry Research Institute, Kyungpook National University) ;
  • Chung, Shin-Kyo (Department of Food Science & Technology, Kyungpook National University)
  • 박진순 (경북대학교 농업생명과학대학 식품공학과) ;
  • 황인욱 (경북대학교 농업생명과학대학 식품공학과) ;
  • 정호철 (경북대학교 농업생명과학대학 식품공학과) ;
  • 김숙경 (경북대학교 식품생물산업연구소) ;
  • 정신교 (경북대학교 농업생명과학대학 식품공학과)
  • Received : 2009.11.23
  • Accepted : 2010.03.05
  • Published : 2010.04.30

Abstract

Acidic hydrolysis conditions prior to HPLC analysis of plant flavonoids was investigated by response surface methodology (RSM), using leaves of Ginkgo biloba L. Ten hydrolysis conditions using 0.5-2.5 M HCl and 0.5-2.5 hr hydrolysis time were chosen to form a central composite rotatable design (CCRD), and optimization by RSM was achieved by measuring myricetin, quercetin, and kaempferol levels by HPLC. Optimum hydrolysis condition was 1.5 M HCl for 1.5 hr. The levels of flavonoids obtained under the condition were in good agreement with predicted maximum values, with yields of more than 95%. These optimum hydrolysis condition was applied to analysis of flavonoid content in the leaves of Camellia sinensis, Ficus carica, and Sageretia theezans.

Acknowledgement

Supported by : 한국학술진흥재단

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