Screening of $\alpha$-amylase and $\alpha$-glucosidase Inhibitors from Brazilian Plant Extracts for Treatment of Rumen Acidosis

100종 브라질 식물 추출물로부터 반추동물 산독증 예방치료를 위한 $\alpha$-amylase 및 $\alpha$-glucosidase 저해제의 선별

  • Kim, Mi-Sun (Department of Food & Nutrition, Andong National University) ;
  • An, Seon-Mi (Department of Food & Nutrition, Andong National University) ;
  • Jung, In-Chang (Department of Food & Nutrition, Andong National University) ;
  • Kwon, Gi-Seok (School of Bioresource Science, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food & Nutrition, Andong National University)
  • 김미선 (안동대학교 식품영양학과) ;
  • 안선미 (안동대학교 식품영양학과) ;
  • 정인창 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 손호용 (안동대학교 식품영양학과)
  • Received : 2010.12.04
  • Accepted : 2010.03.19
  • Published : 2010.04.30

Abstract

To develop anti-acidosis and anti-diabetes agentsfrom natural products, the inhibitory activities of Brazilian plant extracts against microbial $\alpha$-amylase and $\alpha$-glucosidase were evaluated. Among 100 different ethanol extracts tested, those of Acacia jurema Mart., Anacardium humile A. St.-Hil., Cedrela odorata L., and Guazuma ulmifolia Lam showed good inhibitoryactivities toward both enzymes. In addition, an extract of Plumeria drastica Mart. showed specific inhibition of $\alpha$-amylase, whereas that of Eugenia uniflora L. demonstrated strong inhibition of the enzyme. IC50 values of $\alpha$-amylase inhibition suggested that the extract of A. humile A. St.-Hil., which has been used as an anti-diabetes medicine in Brazil, had potent inhibitory activity. The IC50 for the A. humile A. St.-Hil. extract ($91.2{\mu}g/mL$) was similar to that of acarbose ($50.5{\mu}g/mL$). This activity of A. humile A. St.-Hil. was not reduced by heat or acid treatment. Moreover, treatment with HCl (0.01 M) for 1 h increased the inhibitory activity from 57.5% to 81.2%. Also, the extract did not cause hemolysis of human red blood cells at levels up to 1 mg/mL. The results indicate that the extract of A. humile A. St.-Hil. is potentially useful as an anti-acidosis and anti-diabetes agent.

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